Changes in Plasma Concentrations of Immunoreactive Inhibin and Estradiol-17.BETA. in the Golden Hamster Superovulated by Equine Chorionic Gonadotropin (eCG).

Kishi, Hisashi; Okada, Toshiyuki; Otsuka, Mariko; Watanabe, Gen; Taya, Kazuyoshi

doi:10.1262/jrd.43.33

Cited by 3 publications

(2 citation statements)

References 19 publications

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“…In addition, treatment with eCG resulted in a dramatic increase in the secretion of inhibin and estradiol-17␤. These increases were accompanied by a decrease in plasma levels of FSH as observed previously [45][46][47]. Our results indicate that stimulation of follicular development by administration of eCG was thought to induce an increase in plasma inhibin, which in turn suppress FSH secretion.…”

Section: Discussionsupporting

confidence: 88%

A New Alternative Method for Superovulation Using Passive Immunization Against Inhibin in Adult Rats1

Ishigame

Medan

Watanabe

et al. 2004

Biology of Reproduction

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The present study was undertaken to investigate the effects of passive immunoneutralization of endogenous inhibin on ovulation rate and embryo development in vivo and in vitro to establish a new alternative superovulation method in the adult rat. Female adult rats of Wistar strain were superovulated with a single injection of inhibin antiserum (inhibin-AS; 100 or 400 microl) or an injection of 20 IU eCG followed by an injection of 10 IU hCG. Untreated animals served as controls. Embryos were collected from oviducts or uteri on Days 1-5 of pregnancy, and the number of embryos and implantation sites were observed. On Day 1 of pregnancy, the two-cell-stage embryos were cultured and embryos from the 100-microl inhibin-AS group and the control group were transferred to recipient females to determine developmental competence. There were no significant differences between groups in fertilization rate. The numbers of normal embryos in the inhibin-AS-treated groups were significantly higher than the control and the eCG-hCG-treated groups throughout Days 1-4 of pregnancy. The number of implantation sites observed on Day 5 of pregnancy in the inhibin-AS-treated groups was significantly higher than both the control and the eCG-hCG-treated groups. Furthermore, the rate of blastocyst development in vitro in the inhibin-AS-treated groups and posttransfer viability in the 100-microl-inhibin-AS group were comparable with those of the control group. These results indicate that immunoneutralization of endogenous inhibin is a new practical alternative for induction of superovulation as a substitution for eCG-hCG method in the adult rat.

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Section: Discussionsupporting

confidence: 88%

A New Alternative Method for Superovulation Using Passive Immunization Against Inhibin in Adult Rats1

Ishigame

Medan

Watanabe

et al. 2004

Biology of Reproduction

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“…It is conceivable that inhibin controlls FSH secretion in male golden hamsters like rats during sexual maturation. Inhibin is mainly produced by gonads and suppresses FSH secretion from the pituitary gland in adult female [6,7] and male [8] golden hamsters. In adult male golden hamsters, inhibin was produced by not only Sertoli but also by Leydig cells, and the intensity of immunostaining for the inhibin α subunit varied in the seminiferous tubules [8].…”

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confidence: 99%

Developmental Changes in Testicular Function and Inhibin Secretion of Golden Hamsters (<i>Mesocricetus auratus</i>)

Itoh

Kishi

Ohshima

et al. 2002

Journal of Reproduction and Development

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Abstract. We studied developmental reproduction of male golden hamsters (Mesocricetus auratus) by determining hormone secretion, observing morphological changes including distribution of immunoexpression of inhibin α subunit, 3β-hydroxysteroid dehydrogenase (HSD), and investigating sperm head count from birth to adulthood. Immunoexpression of inhibin α subunit was found in interstitial cells of not well organized testes of neonatal animals, and positive staining was also found in Sertoli cells in developing animals. However, the intensity of immunostaining in Sertoli cells varied when the spematogenic cycle of the seminiferous epithelium began. Testosterone levels in the plasma and testicular contents of testosterone and immunoreactive (ir)-inhibin increased from around 25 days of age, about 10 days before the presence of the first sperm in the testis. On the other hand, plasma concentrations of ir-inhibin progressively increased from birth to 10 days of age, when it reached peak levels. A reciprocal pattern of change between plasma concentrations of ir-inhibin and FSH was found from 10 days of age. Plasma concentrations and pituitary contents of LH increased from 15 days of age and reached adult levels about 40 days of age. The present results suggest that inhibin is an important factor in the regulation of FSH secretion even in infant male golden hamsters, and the regulated FSH may control the increasing the number of germ cells. Inhibin might be not the only endocrine factor as the regulator of FSH secretion, but also a paracrine or autocrine factor which is involved in spermatogenesis in the golden hamster. Key words: Inhibin, Hamster, Development, Testis (J. Reprod. Dev. 48: [343][344][345][346][347][348][349][350][351][352][353] 2002) arly 20 th century experiments, which were the initial proposal for the existence of inhibin, showed that destruction of the germinal epithelium by irradiation caused hyperfunction in the absence of atrophy of the second sex glands [1], and that injection of aqueous testicular extracts to castrated rats prevented the appearance of enlarged "castration cells" in the pituitary [2,3]. These

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Changes in Expression of Inhibin Subunits in the Cyclic Golden Hamster (Mesocricetus auratus) and the Regulation of Inhibin α Subunit Production by Luteinizing Hormone

Kishi

Ohshima²,

Itoh

et al. 2002

Zoological Science

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In the present study, changes in localization of each inhibin subunit in the ovary were investigated during the estrous cycle of the golden hamster. The effect of LH surge on changes in localization in inhibin alpha subunit in the ovary was also investigated. Inhibin alpha subunit was localized in granulosa cells of various stages of follicles throughout the estrous cycle. Inhibin alpha subunit was also present in numerous interstitial cells on days 1 and 2 (day 1 = day of ovulation), but the number of positive interstitial cells was fewer on days 3 and almost disappeared on day 4 of the estrous cycle. Newly formed luteal cells were also positive for inhibin alpha subunit on days 1 and 2. On the other hand, positive reactions for inhibin beta A and beta B subunits were only present in the granulosa cells of healthy antral follicles. However, a positive reaction for inhibin beta B subunit in peripheral mural granulosa cells disappeared on days 3 and 4 of the estrous cycle. Treatment with LHRH-AS at 1100 h on day 4 completely blocked the luteinizing hormone (LH) surge and ovulation, although relatively high concentrations of plasma follicle-stimulating hormone (FSH) were maintained throughout the experiment. There were few positive reactions for inhibin alpha subunit in theca and interstitial cells 24 hr after LHRH-AS injection. The effect of LHRH-AS treatment was blocked by a single injection of 10 IU human chorionic gonadotropin. These results suggest that the major source of dimeric inhibin in the cyclic hamster was granulosa cells of healthy antral follicles. Different distribution pattern of inhibin beta A from beta B subunits in large antral follicles on days 3 and 4 of the estrous cycle suggests different secretion patterns of inhibin A from B on these days. Furthermore, the LH surge may be an important factor to induce production of inhibin alpha subunit in interstitial cells of the cyclic hamster.

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Changes in Plasma Concentrations of Immunoreactive Inhibin and Estradiol-17.BETA. in the Golden Hamster Superovulated by Equine Chorionic Gonadotropin (eCG).

Cited by 3 publications

References 19 publications

A New Alternative Method for Superovulation Using Passive Immunization Against Inhibin in Adult Rats1

A New Alternative Method for Superovulation Using Passive Immunization Against Inhibin in Adult Rats1

Developmental Changes in Testicular Function and Inhibin Secretion of Golden Hamsters (<i>Mesocricetus auratus</i>)

Changes in Expression of Inhibin Subunits in the Cyclic Golden Hamster (Mesocricetus auratus) and the Regulation of Inhibin α Subunit Production by Luteinizing Hormone

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