Changes in physiological states of Salmonella Typhimurium measured by qPCR with PMA and DyeTox13 Green Azide after pasteurization and UV treatment

Li, Liyan; Fu, Jing; Bae, Sung Woo

doi:10.1007/s00253-022-11850-0

Cited by 6 publications

(4 citation statements)

References 40 publications

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“…(Trieu & Lee, 2019). Given that dye‐based PCR detection is dependent on membrane permeability, this method may not be applicable for assessing bacteria inactivated by processing treatments that do not compromise membrane integrity (e.g., ultraviolet) (Li et al., 2022; Techathuvanan & D'Souza, 2020). Uptake of PMA by viable cells can also lead to false negative results (Emerson et al., 2017; Kumar & Ghosh, 2019).…”

Section: Nucleic Acid‐based Amplification Methods For Targeted Detect...mentioning

confidence: 99%

Molecular detection and characterization of foodborne bacteria: Recent progresses and remaining challenges

Hadi

Rapp

Dhawan

et al. 2023

Comp Rev Food Sci Food Safe

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The global food demand is expected to increase in the coming years, along with challenges around climate change and food security. Concomitantly, food safety risks, particularly those related to bacterial pathogens, may also increase. Thus, the food sector needs to innovate to rise to this challenge. Here, we discuss recent advancements in molecular techniques that can be deployed within various foodborne bacteria surveillance systems across food settings. To start with, we provide updates on nucleic acid-based detection, with a focus on polymerase chain reaction (PCR)-based technologies and loop-mediated isothermal amplification (LAMP). These include descriptions of novel genetic markers for several foodborne bacteria and progresses in multiplex PCR and droplet digital PCR.The next section provides an overview of the development of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) proteins systems, such as CRISPR-Cas9, CRISPR-Cas12a, and CRISPR-Cas13a, as tools for enhanced sensitive and specific detection of foodborne pathogens. The final section describes utilizations of whole genome sequencing for accurate characterization of foodborne bacteria, ranging from epidemiological surveillance to model-based predictions of bacterial phenotypic traits through genome-wide association studies or machine learning.

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Section: Nucleic Acid‐based Amplification Methods For Targeted Detect...mentioning

confidence: 99%

Molecular detection and characterization of foodborne bacteria: Recent progresses and remaining challenges

Hadi

Rapp

Dhawan

et al. 2023

Comp Rev Food Sci Food Safe

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show abstract

“…By comparing the average ΔCt values of PCR reactions treated with the three dyes, there was no significant difference in the detection results, indicating that their ability to identify active bacteria in ordinary samples is equivalent. Li et al [ 16 ] detected viable and dead bacteria in UV disinfected samples using PMA-PCR and DyeTox13-PCR. Different from the results of the PMA test where only “membrane damaged” cells can be distinguished, the DyeTox13 test showed greater ability on “dormant” cells with no metabolic activity in eggshell samples, which was consistent with the results of plate counting, indicating it is more suitable to detect viable bacteria in samples after UV irradiation ( Figure 2 C).…”

Section: Dna-based Viability Pcr Detecting Techniquementioning

confidence: 99%

“… Schematic diagram of four nucleic acid intercalation dyes combined with PCR for distinguishing and detecting viable and dead bacteria. ( A ) Principal diagram of EMA-PCR for detecting viable and dead bacteria based on cell membrane integrity [ 10 ]; ( B ) Flow chart of multiple real-time detection of viable bacteria by PMA-PCR [ 14 ]; ( C ) Flow chart of DyeTox13-PCR detection of viable bacteria in sterilized samples [ 16 ]; ( D ) Principal diagram of TOMA-PCR for detecting viable and dead bacteria based on enzyme activity [ 18 ]. …”

Section: Figurementioning

confidence: 99%

PCR Mediated Nucleic Acid Molecular Recognition Technology for Detection of Viable and Dead Foodborne Pathogens

Chen

Lan

Zhu

et al. 2022

Foods

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Living foodborne pathogens pose a serious threat to public and population health. To ensure food safety, it is necessary to complete the detection of viable bacteria in a short time (several hours to 1 day). However, the traditional methods by bacterial culture, as the gold standard, are cumbersome and time-consuming. To break through the resultant research bottleneck, PCR mediated nucleic acid molecular recognition technologies, including RNA-based reverse transcriptase PCR (RT-PCR) and DNA-based viability PCR (vPCR) have been developed in recent years. They not only sensitively amplify detection signals and quickly report detection results, but also distinguish viable and dead bacteria. Therefore, this review introduces these PCR-mediated techniques independent of culture for viable and dead foodborne pathogen detection from the nucleic acid molecular recognition principal level and describes their whole-process applications in food quality supervision, which provides a useful reference for the development of detection of foodborne pathogens in the future.

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“…In the past two decades, a number of other methods had been developed for differentiating dead bacteria from live/dead cell mixtures, including electron microscopy, − real-time fluorescent quantitative PCR (qPCR), surface-enhanced Raman scattering, aggregation-induced emission (AIE), and fluorescent techniques combined with fluorescence spectra, , optical microscopy, − or flow cytometry (FCM). , However, none of these techniques meets the requirements of both high sensitivity and fast detection. For instance, fluorescence spectra can hardly determine the accurate death rate of bacteria that are treated by antibiotics .…”

Section: Introductionmentioning

confidence: 99%

Rapid and Sensitive Quantification of Bacterial Viability Using Ratiometric Fluorescence Sensing

He,

Chen,

Wang

et al. 2024

Anal. Chem.

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Changes in physiological states of Salmonella Typhimurium measured by qPCR with PMA and DyeTox13 Green Azide after pasteurization and UV treatment

Cited by 6 publications

References 40 publications

Molecular detection and characterization of foodborne bacteria: Recent progresses and remaining challenges

Molecular detection and characterization of foodborne bacteria: Recent progresses and remaining challenges

PCR Mediated Nucleic Acid Molecular Recognition Technology for Detection of Viable and Dead Foodborne Pathogens

Rapid and Sensitive Quantification of Bacterial Viability Using Ratiometric Fluorescence Sensing

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