Changes in photosynthetic capacity and antioxidant enzymatic systems in micropropagated Zingiber officinale plantlets during their acclimation

Guan, Qiuzhu; Guo, Yinghua; Sui, X. L.; Li, W.; Zhang, Z. X.

doi:10.1007/s11099-008-0031-y

Cited by 20 publications

(14 citation statements)

References 33 publications

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“…In Solanum tuberosum (Baroja et al 1995), Spathiphyllum floribundum (Van Huylenbroeck and Debergh 1996), Rehmania glutinosa (Seon et al 2000), Capsicum annuum (Estrada-Luna et al 2001), Ocimum basilicum (Siddique and Anis 2008) and Zingiber officinale (Guan et al 2008) P N also decreased in the first days after transplantation and increased thereafter. In present experiments we measured persistent leaves, however, significant increase in P N to values higher than during in vitro growth was usually found only when new leaves were fully developed (e.g.…”

Section: Resultsmentioning

confidence: 99%

“…Exposure of Calathea louisae and Spathiphyllum floribundum plantlets to high irradiance immediately after transplantation caused photoinhibition and even Chl photobleaching (Van Huylenbroeck 1994, Van Huylenbroeck et al 1995 but no photoinhibition was observed in plants acclimatized under low irradiance (Van Huylenbroeck 1994). Similarly, photoinhibition characterized by decrease in variable to maximum fluorescence ratio (F v /F m ) was observed in Rosa hybrida and Zingiber officinale plantlets in the first week after ex vitro transfer (Sallanon et al 1998, Genoud et al 1999, Guan et al 2008. Immediately after ex vitro transfer, reversible photoinhibition was observed in Castanea sativa and Vitis vinifera (Carvalho et al 2001).…”

Section: Introductionmentioning

confidence: 96%

“…P N usually decreased in the first days after transplantation and increased thereafter (e.g. Van Huylenbroeck and Debergh 1996, Kadleček et al 1998, Seon et al 2000, Estrada-Luna et al 2001, Guan et al 2008. Substantial increase in P N was measured when new leaves were fully developed (3 -4 weeks after ex vitro transfer) (e.g.…”

Section: Introductionmentioning

confidence: 99%

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Effect of abscisic acid on photosynthetic parameters during ex vitro transfer of micropropagated tobacco plantlets

Pospíšilová¹,

Synková²,

Haisel³

et al. 2009

Biologia plant.

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The aim of this research was to determine whether exogenous abscisic acid (ABA) applied immediately after ex vitro transfer of in vitro grown plants can improve their acclimatization. Tobacco (Nicotiana tabacum L.) plantlets were transferred into pots with Perlite initially moistened either by water or 50 μM ABA solution and they were grown under low (LI) or high (HI) irradiance of 150 and 700 μmol m -2 s -1 , respectively. Endogenous content of ABA in tobacco leaves increased considerably after ABA application and even more in plants grown under HI. Stomatal conductance, transpiration rate and net photosynthetic rate decreased considerably 1 d after ex vitro transfer and increased thereafter. The gas exchange parameters were further decreased by ABA application and so wilting of these plants was limited. Chlorophyll (a+b) and β-carotene contents were higher in ABA-treated plants, but the content of xanthophyll cycle pigments was not increased. However, the degree of xanthophyll cycle pigments deepoxidation was decreased what also suggested less stress in ABA-treated plants. No dramatic changes in most chlorophyll a fluorescence parameters after ex vitro transfer suggested that the plants did not suffer from restriction of electron transport or photosystem damage.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

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Effect of abscisic acid on photosynthetic parameters during ex vitro transfer of micropropagated tobacco plantlets

Pospíšilová¹,

Synková²,

Haisel³

et al. 2009

Biologia plant.

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“…Based on several reports (Rout et al, 2001, Guo and Zhang, 2005, Ma and Gang, 2006, Guo et al, 2007, Guan et al, 2008and Zheng et al, 2008 ginger, is constrained severely can summarized as following: (1) ginger normally propagates by its rhizome, with a low proliferation rate, and the reproducing part (the rhizome) is also the economically used part of the ginger plant, which restricts the availability of ginger seeds needed for cultivation, (2) easily infected by soil-born pathogens such as bacterial wilt (Pseudomonas solanacearum), soft rot (Pythium aphanidermatum), and nematodes (Meloidogyne spp. ), which cause heavy losses in yield.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Production of Microrhizomes in Ginger (Zingiber Officinale Rosco)

Abbas¹,

Aly²,

Taha³

et al. 2014

J microb biotech food sci

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The present investigation was carried out to highlight an effective protocol for in vitro production of ginger microrhizomes. Microrhizomes were induced at the base of the in vitro derived shoots upon transfer to MS medium containing various concentrations of (30, 60 and 90 g/L), BAP: 6-benzylaminopurine (3, 6 and 9 mg/L) and grown under varying photoperiodism in addition to the MS medium supplemented with 9 mg/L BAP and 60-90 g/L sucrose under 16-h photoperiod within 10 weeks of cultivation were the best conditions for microrhizomes induction. Ginger microrhizomes formation in vitro was found to be controlled by many factors, including the concentrations of BAP and sucrose as well as photoperiodism during culturing period.

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“…P N was higher in plants grown under HI than under LI. In several plant species, P N also decreased during the first days after transplantation and increased thereafter but significant increase in P N to values higher than during in vitro growth was usually found only when new leaves were fully developed (see review , and recent papers Siddique and Anis 2008, Guan et al 2008). No statistically significant effect of ABA was found in plantlets grown in vitro and also 1 d after ex vitro transfer while 7 d after ex vitro transfer P N was lower in ABA-treated plants than in control plants (Fig.…”

Section: Resultsmentioning

confidence: 99%

Improvement of ex vitro transfer of tobacco plantlets by addition of abscisic acid to the last subculture

Pospíšilová¹,

Haisel²,

Synková³

et al. 2009

Biol. plant.

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Tobacco (Nicotiana tabacum L.) plantlets were grown on Murashige and Skoog medium in ventilated Magenta boxes and for the last subculture 10 μM ABA was added to the medium. After three weeks plantlets were transferred into pots with Perlite moistened with water and grown in controlled conditions (16-h photoperiod, day/night temperature 25/20 °C, air humidity about 45 %) either under low or high irradiance of 150 (LI) and 700 (HI) μmol m -2 s -1 , respectively. Content of endogenous ABA was 271.7 pmol g -1 (f.m.) in ABA treated plantlets, while in control plantlets it was only 53.3 pmol g -1 (f.m.). After ex vitro transfer, stomatal conductance and transpiration rate decreased considerably in comparison with in vitro grown plantlets and remained lower also 7 d after ex vitro transfer, especially in ABA-treated plants and so wilting of plants was practically eliminated. Net photosynthetic rate also decreased 1 d after ex vitro transfer but after 7 d it was mostly higher than that of in vitro grown plantlets. Water use efficiency significantly increased in ABA-treated plants. Chlorophyll a+b content did not change immediately after ex vitro transfer, nevertheless, after 7 d chlorophyll content was higher in ABA-treated plants. Pool of xanthophyll cycle pigments (XCP) and the degree of their deepoxidation (DEPS), which are connected with harmless dissipation of light energy, increased under high irradiance. Contents of XCP and ABA precursors (neoxanthin and violaxanthin) were lower in ABA-treated plants than in control plants indicating less stress in these plants. Most chlorophyll a fluorescence parameters did not change considerably after ex vitro transfer and so the photoinhibition was not observed even under HI. Slight increase in non-photochemical quenching under HI in ABA-treated plants suggested their better photoprotection. Thus application of ABA to the last subculture can improve acclimatization of in vitro grown plants to ex vitro conditions

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Changes in photosynthetic capacity and antioxidant enzymatic systems in micropropagated Zingiber officinale plantlets during their acclimation

Cited by 20 publications

References 33 publications

Effect of abscisic acid on photosynthetic parameters during ex vitro transfer of micropropagated tobacco plantlets

Effect of abscisic acid on photosynthetic parameters during ex vitro transfer of micropropagated tobacco plantlets

In Vitro Production of Microrhizomes in Ginger (Zingiber Officinale Rosco)

Improvement of ex vitro transfer of tobacco plantlets by addition of abscisic acid to the last subculture

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