Changes in Photosynthetic Activity and Export of Carbon by Overexpressing a Maize Sucrose-Phosphate Synthase Gene under Elevated C0<sub>2</sub>in Transgenic Rice

Ono, Kiyomi; Sasaki, Hidetada; Hara, Takahiro; Kobayashi, Kazuhiko; Ishimaru, Ken

doi:10.1626/pps.6.281

Cited by 11 publications

(3 citation statements)

References 21 publications

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“…These findings are consistent with other reports in the literature (Galtier et al 1995;Signora et al 1998;Ono et al 1999Ono et al , 2003Baxter et al 2003;Strand et al 2003;Lundmark et al 2006;Ramon et al 2008;Stitt et al 2010). The leaves of the transformants showed an intense bluish green color accompanied by an increase in chlorophyll (Chl) content with a more significant increase in Chl b level resulting in a significant drop in the Chl a:b ratio.…”

Section: Discussionsupporting

confidence: 92%

Transgenic alfalfa (Medicago sativa) with increased sucrose phosphate synthase activity shows enhanced growth when grown under N2-fixing conditions

Gebril

Seger

Muro-Villanueva

et al. 2015

Planta

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Overexpression of SPS in alfalfa is accompanied by early flowering, increased plant growth and an increase in elemental N and protein content when grown under N2-fixing conditions. Sucrose phosphate synthase (SPS; EC 2.3.1.14) is the key enzyme in the synthesis of sucrose in plants. The outcome of overexpression of SPS in different plants using transgenic approaches has been quite varied, but the general consensus is that increased SPS activity is associated with the production of new sinks and increased sink strength. In legumes, the root nodule is a strong C sink and in this study our objective was to see how increasing SPS activity in a legume would affect nodule number and function. Here we have transformed alfalfa (Medicago sativa, cv. Regen SY), with a maize SPS gene driven by the constitutive CaMV35S promoter. Our results showed that overexpression of SPS in alfalfa, is accompanied by an increase in nodule number and mass and an overall increase in nitrogenase activity at the whole plant level. The nodules exhibited an increase in the level of key enzymes contributing to N assimilation including glutamine synthetase and asparagine synthetase. Moreover, the stems of the transformants showed higher level of the transport amino acids, Asx, indicating increased export of N from the nodules. The transformants exhibited a dramatic increase in growth both of the shoots and roots, and earlier flowering time, leading to increased yields. Moreover, the transformants showed an increase in elemental N and protein content. The overall conclusion is that increased SPS activity improves the N status and plant performance, suggesting that the availability of more C in the form of sucrose enhances N acquisition and assimilation in the nodules.

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Section: Discussionsupporting

confidence: 92%

Transgenic alfalfa (Medicago sativa) with increased sucrose phosphate synthase activity shows enhanced growth when grown under N2-fixing conditions

Gebril

Seger

Muro-Villanueva

et al. 2015

Planta

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“…Modification of SPS activity by overexpressing or suppressing SPS genes in plants generally alters the ratio of sucrose to starch and carbon partitioning into the sink (Chen et al 2005;Galtier et al 1993;Lundmark et al 2006;Park et al 2008;Signora et al 1998;Strand et al 2000) and crop yield (Laporte et al 2001;Ono et al 2003). However, how changes in SPS expression affect starch levels and turnover, and plant growth, is not as consistent.…”

Section: Introductionmentioning

confidence: 99%

Decrease in leaf sucrose synthesis leads to increased leaf starch turnover and decreased RuBP regeneration‐limited photosynthesis but not Rubisco‐limited photosynthesis in Arabidopsis null mutants of SPSA1

Sun

Zhang

Larue

et al. 2011

Plant Cell & Environment

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“…2003), tobacco (Baxter et al . 2003) and rice (Ono et al . 2003) indicates that SPS activity and Suc export rates may be closely linked.…”

Section: Introductionmentioning

confidence: 99%

Carbon partitioning and export in transgenic Arabidopsis thaliana with altered capacity for sucrose synthesis grown at low temperature: a role for metabolite transporters

Lundmark

Cavaco

Trevanion

et al. 2006

Plant Cell & Environment

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We investigated the role of metabolite transporters in cold acclimation by comparing the responses of wild-type (WT) Arabidopsis thaliana (Heynh.) with that of transgenic plants over-expressing sucrose-phosphate synthase (SPSox) or with that of antisense repression of cytosolic fructose-1,6-bisphosphatase (FBPas). Plants were grown at 23 ∞ C and then shifted to 5 ∞ C. We compared the leaves shifted to 5 ∞ C for 3 and 10 d with new leaves that developed at 5 ∞ C with control leaves on plants at 23 ∞ C. At 23 ∞ C, ectopic expression of SPS resulted in 30% more carbon being fixed per day and an increase in sucrose export from source leaves. This increase in fixation and export was supported by increased expression of the plastidic triose-phosphate transporter AtTPT and, to a lesser extent, the high-affinity Suc transporter AtSUC1. The improved photosynthetic performance of the SPSox plants was maintained after they were shifted to 5 ∞ C and this was associated with further increases in AtSUC1 expression but with a strong repression of AtTPT mRNA abundance. Similar responses were shown by WT plants during acclimation to low temperature and this response was attenuated in the low sucrose producing FBPas plants. These data suggest that a key element in recovering flux through carbohydrate metabolism in the cold is to control the partitioning of metabolites between the chloroplast and the cytosol, and Arabidopsis modulates the expression of AtTPT to maintain balanced carbon flow. Arabidopsis also up-regulates the expression of AtSUC1, and to lesser extent AtSUC2, as down-stream components facilitate sucrose transport in leaves that develop at low temperatures.

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Changes in Photosynthetic Activity and Export of Carbon by Overexpressing a Maize Sucrose-Phosphate Synthase Gene under Elevated C0₂in Transgenic Rice

Cited by 11 publications

References 21 publications

Transgenic alfalfa (Medicago sativa) with increased sucrose phosphate synthase activity shows enhanced growth when grown under N2-fixing conditions

Transgenic alfalfa (Medicago sativa) with increased sucrose phosphate synthase activity shows enhanced growth when grown under N2-fixing conditions

Decrease in leaf sucrose synthesis leads to increased leaf starch turnover and decreased RuBP regeneration‐limited photosynthesis but not Rubisco‐limited photosynthesis in Arabidopsis null mutants of SPSA1

Carbon partitioning and export in transgenic Arabidopsis thaliana with altered capacity for sucrose synthesis grown at low temperature: a role for metabolite transporters

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Changes in Photosynthetic Activity and Export of Carbon by Overexpressing a Maize Sucrose-Phosphate Synthase Gene under Elevated C02in Transgenic Rice

Cited by 11 publications

References 21 publications

Transgenic alfalfa (Medicago sativa) with increased sucrose phosphate synthase activity shows enhanced growth when grown under N2-fixing conditions

Transgenic alfalfa (Medicago sativa) with increased sucrose phosphate synthase activity shows enhanced growth when grown under N2-fixing conditions

Decrease in leaf sucrose synthesis leads to increased leaf starch turnover and decreased RuBP regeneration‐limited photosynthesis but not Rubisco‐limited photosynthesis in Arabidopsis null mutants of SPSA1

Carbon partitioning and export in transgenic Arabidopsis thaliana with altered capacity for sucrose synthesis grown at low temperature: a role for metabolite transporters

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Changes in Photosynthetic Activity and Export of Carbon by Overexpressing a Maize Sucrose-Phosphate Synthase Gene under Elevated C0₂in Transgenic Rice