2002
DOI: 10.1074/jbc.m107899200
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Changes in Phospholipid Extractability and Composition Accompany Mineralization of Chicken Growth Plate Cartilage Matrix Vesicles

Abstract: Matrix vesicles are lipid bilayer-enclosed structures that initiate extracellular mineral formation. Little attention has been given to how newly formed mineral interacts with the lipid constituents and then emerges from the lumen. To explore whether specific lipids bind to the incipient mineral and if breakdown of the membrane is involved, we analyzed changes in lipid composition and extractability during vesicle-induced calcification. Isolated matrix vesicles were incubated in synthetic cartilage lymph to in… Show more

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Cited by 82 publications
(110 citation statements)
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“…2 Some suggested mechanisms of action for the PUFAs in these models include the following: modulation of calcium absorption from the intestine, increased bone mineralization, enhancement of bone collagen synthesis, and reduction of urinary calcium excretion, thereby increasing the pool of available calcium for bone mineralization in the body. 1,4,5,12,13 Others have shown that acidic phospholipids facilitate cartilage mineralization in the growth plate, 15 while prostaglandins, which are synthesized from fatty acids, affect insulin-like growth factors and bone formation in animals. 16,17 The effects of PUFAs at the cellular level are not fully understood.…”
Section: Introductionmentioning
confidence: 99%
“…2 Some suggested mechanisms of action for the PUFAs in these models include the following: modulation of calcium absorption from the intestine, increased bone mineralization, enhancement of bone collagen synthesis, and reduction of urinary calcium excretion, thereby increasing the pool of available calcium for bone mineralization in the body. 1,4,5,12,13 Others have shown that acidic phospholipids facilitate cartilage mineralization in the growth plate, 15 while prostaglandins, which are synthesized from fatty acids, affect insulin-like growth factors and bone formation in animals. 16,17 The effects of PUFAs at the cellular level are not fully understood.…”
Section: Introductionmentioning
confidence: 99%
“…Two of the main lipids found in the MV membranes are dipalmitoylphosphatidylserine and dipalmitoylphosphatidylcholine (DPPC), and many studies have revealed that they play a crucial role in the biomineralization process, regulating both calcium entry into the MVs and formation of HA crystals (Kirsch et al 1997;Wu et al 2002). Using the direct insertion method and DPPC liposomes, Simão et al (2010a) described the production and characterization of proteoliposomes harboring TNAP alone, NPP1 alone, and TNAP + NPP1 together as MV biomimetics to further understand the interplay between these enzymes in the utilization of physiological substrates as a means of understanding the more complex interplay of these enzymes in intact MVs, crucial during early events of skeletal mineralization.…”
Section: New Perspectives For Applications Of Proteoliposomesmentioning
confidence: 99%
“…In order to understand the physiological interplay between important MV-associated enzymes in the initiation of biomineralization, it is important to keep in mind the microenvironment in which these enzymes function, which can have a profound effect on their biological properties, since phospholipids play an important role in the initiation of the biomineralization process (Anderson et al 1997;Wu et al 2002). Early studies of MV lipid composition have determined that there are significant differences between the lipid content of MVs and the plasma membranes from which they arose.…”
Section: New Perspectives For Applications Of Proteoliposomesmentioning
confidence: 99%
“…The nonpolar lipids were from Sigma and included free cholesterol (5-cholesten-3-ol, CH) and cholesterol oleate (5-cholesten-3␤-ol 3-oleate). Using methods just described, various lipid-Ca 2ϩ -P i CPLXs were formed from either PS alone, PS and PE in equimolar amounts, or from equimolar amounts of PS, PE, PC, CH, and CHE, the latter mimicking the lipid composition of MV (47,48).…”
Section: Synthesis Of Acp Cplx and Anx-a5-containingmentioning
confidence: 99%
“…However, in models of the nucleational core that lack Anx-A5, PE has been shown to strongly inhibit the ability of PS-CPLX to induce mineral formation (32). Interestingly, during the process of MV mineralization, PE and several other non-Ca 2ϩ -binding lipids become extensively degraded (48). In contrast, PS becomes complexed with the newly forming mineral, and its levels increase, apparently at the expense of PE by base-exchange with free serine (49).…”
mentioning
confidence: 99%