Changes in phosphatase activity in phosphorus-deficient Spirodela

Reid, M; Bieleski, R. L.

doi:10.1007/bf00385759

Cited by 58 publications

(29 citation statements)

References 15 publications

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“…Conditions of growth, the course and symptoms of P deficiency (Bieleski 1968), and the development of phosphatase activity (Reid and Bieleski 1970) were as previously reported.…”

Section: (A) Plant Materialsmentioning

confidence: 89%

“…Proteins in extracts were separated by flat-plate gel electrophoresis (Reid and Bieleski 1970). Afterwards, gel plates were washed in imidazole buffer, pH 6·4 (30 min, three changes), then one strip was cut from each plate and stained with ",-naphthyl phosphate (0'005%) plus fast red tetrazolium salt (0'005%) (Burstone 1962) to reveal phosphatase.…”

Section: (D) Gel Electrophoresi8mentioning

confidence: 99%

“…Afterwards, gel plates were washed in imidazole buffer, pH 6·4 (30 min, three changes), then one strip was cut from each plate and stained with ",-naphthyl phosphate (0'005%) plus fast red tetrazolium salt (0'005%) (Burstone 1962) to reveal phosphatase. Areas in the unstained gel corresponding to regions containing phosphatase were assayed for phosphatase activity using p-nitrophenyl phosphate as substrate (Reid and Bieleski 1970).…”

Section: (D) Gel Electrophoresi8mentioning

confidence: 99%

“…During P deficiency, phosphatase activity increases and two low molecular weight isoenzymes of phosphatase appear (Reid and Bieleski 1970). Although the root shows the most marked increase in phosphatase activity, the ratio of low molecular weight to high molecular weight isoenzyme activity is much lower in the root than in the fronds (Table 2).…”

Section: (B) Phosphatase I Soenzymes In Root and Leafmentioning

confidence: 99%

“…is grown in a minusphosphorus medium, characteristic symptoms of phosphorus deficiency (P deficiency) develop (Bieleski 1968), notably a 10-50-fold increase in acid phosphatase activity (Reid and Bieleski 1970). Part of this increased activity is due to the appearance in P-deficient tissue of two new isoenzymes of lower molecular weight.…”

Section: Introductionmentioning

confidence: 99%

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The External Location of Phosphatase Activity in Phosphorus-Deficient Spirodela Oligorrhiza

Bieleski¹,

Johnson²

1972

Aust. Jnl. Of Bio. Sci.

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Ab8tractPhosphorus deficiency (P deficiency) caused a 10-20·fold increase in phosphatase activity of S. oligorrhiza homogenates. Specific staining showed that phosphatase in control plants was located primarily in and around the vascular strands, and in P·deficient plants it was located in the epidermis of the root and undersurface of the frond. Dissection experiments showed that roots of P·deficient plants contained a disproportionately high phosphatase activity. When plant and root homogenates were fractionated, phosphatase activity was recovered in the supernatant rather than in any cell particle fraction. However, intact plants released very little phosphatase to the external medium.Intact plants were placed in solutions containing phosphatase substrates. Control plants hydrolysed the substrates at 4-8% of the rates of comparable homogenates. P·deficient plants hydrolysed substrate at 30-40% of the homogenate rate. When glucose 1.[32P]phosphate was supplied to intact plants, more 32p; appeared in the medium than in the tissue. The pattern of appearance was con· sistent with hydrolysis of glucose 1.[32P]phosphate in the external medium followed by accumulation of 32P! into the tissue; and was not consistent with hydrolysis of glucose 1-[32P]phosphate in the tissue followed by loss of 32p; to the medium. We conclude that a large part of the phosphatase which arises in the plant S. oligorrhiza under the stress of P deficiency is located in either the cell wall or the external membrane, where its function is to utilize phosphate esters released to the medium by dying plants. Although a new isoenzyme appears during P deficiency it does not appear to function specifically as the externally active phosphatase.

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“…Conditions of growth, the course and symptoms of P deficiency (Bieleski 1968), and the development of phosphatase activity (Reid and Bieleski 1970) were as previously reported.…”

Section: (A) Plant Materialsmentioning

confidence: 89%

Section: (D) Gel Electrophoresi8mentioning

confidence: 99%

Section: (D) Gel Electrophoresi8mentioning

confidence: 99%

Section: (B) Phosphatase I Soenzymes In Root and Leafmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The External Location of Phosphatase Activity in Phosphorus-Deficient Spirodela Oligorrhiza

Bieleski¹,

Johnson²

1972

Aust. Jnl. Of Bio. Sci.

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Nutrient imbalances in tomato plants and acid phosphatase activity in the leaves

Besford¹

1979

J Sci Food Agric

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Tomato plants were grown in sand for 5 weeks and received one of fifteen different nutrient solutions in which the concentrations of various elements were either reduced or increased individually, relative to their concentration in a complete nutrient solution, and the acid phosphatase activity in extracts from the young, fully-expanded leaves was determined. When expressed on a fresh weight basis, only in the P-deficient plants was the activity significantly increased with a degree of confidence better than P=O.O5; a slight increase occurred in extracts from the plants suffering from B toxicity, but this was only significant at P=O.1. There were no increases in acid phosphatase activity with deficiencies of N, K, Ca, Mg, B, Cu, Fe, Mn, Mo or Zn; or with toxicities of Cu, Mn or Zn. When expressed on a protein N basis, only N and P deficiencies increased the acid phosphatase activity. It is proposed that the acid phosphatase activity of the leaves could be used as an indicator of P deficiency in the tomato.

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Phosphorus nutrition and acid phosphatase activity in the leaves of seven plant species

Besford¹

1979

J Sci Food Agric

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Seven plant species, barley, cucumber, maize, oats, rice, tomato and wheat were grown in sand for between 9 and 11 weeks and received either 1.33 m~-NaHzP04 in a complete nutrient solution (Pz), or 0.13 ~M -N~H z P O~ for 7 days then no added phosphate in an otherwise complete nutrient solution (PI). At harvest plants grown in the PI solution were much smaller than in the Pz solution, the rice showing the least effect, but only the leaves of cucumber and tomato developed characteristic symptoms of phosphorus deficiency. Acid phosphatase activity was significantly greater in extracts from the leaves of all species grown in the PI, except for rice, when compared with those grown in the Pz solution. The increase was greatest in the leaves of tomato and cucumber which also contained the lowest levels of phosphorus.Acid phosphatase activity, therefore, may be useful as a rapid indicator of phosphorus stress in crops.

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Changes in phosphatase activity in phosphorus-deficient Spirodela

Cited by 58 publications

References 15 publications

The External Location of Phosphatase Activity in Phosphorus-Deficient Spirodela Oligorrhiza

The External Location of Phosphatase Activity in Phosphorus-Deficient Spirodela Oligorrhiza

Nutrient imbalances in tomato plants and acid phosphatase activity in the leaves

Phosphorus nutrition and acid phosphatase activity in the leaves of seven plant species

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