Changes in peripheral blood lymphocyte phenotypes distribution in patients with oral cancer/oral leukoplakia in Taiwan

Lee, Jang‐Jaer; Lin, Chungwei; Chen, Chien-Jen; Kok, Sang‐Heng; Chang, Mei Chi; Jeng, Jiiang‐Huei

doi:10.1016/j.ijom.2010.04.045

Cited by 13 publications

(14 citation statements)

References 41 publications

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“…In line with our ndings, others also observed a decrease in the T helper subset in head and neck cancer patients during and after de nite and adjuvant RT [11,15,22]. As patients with recurrent SCCHN are more likely to display abnormalities in their T cell development [23], the drop in CD4 + T cells before start of therapy (t0) appeared to be more pronounced in patients with locoregional recurrence compared to patients who remained free of recurrence [22][23][24]. Therefore, we hypothesize that low initial T helper counts might provide a potential predictive marker for patients that more likely will develop a recurrent disease.…”

Section: Discussionsupporting

confidence: 85%

Comparison of the Composition of Lymphocyte Subpopulations in Non-relapse and Relapse Patients With Squamous Cell Carcinoma of the Head and Neck Before, During Radiochemotherapy and in the Follow-up Period: a Multicenter Prospective Study of the German Cancer Consortium Radiation Oncology Group (DKTK-ROG)

Niu

Combs

Linge

et al. 2020

Preprint

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Background: Radiochemotherapy (RCT) has been shown to induce changes in immune cell homeostasis which might affect antitumor immune responses. In the present study, we aimed to compare the composition and kinetics of major lymphocyte subsets in the periphery of patients with non-locoregional recurrent (n=23) and locoregional recurrent (n=9) squamous cell carcinoma of the head and neck (SCCHN) upon primary RCT.Methods: EDTA-blood of non-locoregional recurrent SCCHN patients was collected before (t0), after application of 20 to 30 Gy (t1), in the follow-up period 3 (t2) and 6 months (t3) after RCT. In patients with locoregional recurrence blood samples were taken at t0, t1, t2 and at the time of recurrence (t5). EDTA-blood of age-related, healthy volunteers (n=22) served as a control (Ctrl). Major lymphocyte subpopulations were phenotyped by multiparameter flow cytometry.Results: Patients with non-recurrent SCCHN had significantly lower proportions of CD19+ B cells compared to healthy individuals before start of any therapy (t0) that dropped further until 3 months after RCT (t2), but reached initial levels 6 months after RCT (t3). The proportion of CD3+ T and CD3+/CD4+ T helper cells continuously decreased between t0 and t3, whereas that of CD8+ cytotoxic T cells and CD3+/CD56+ NK-like T cells (NKT) gradually increased in the same period of time in non-recurrent patients. The percentage of CD4+/CD25+/FoxP3+ regulatory T cells (Tregs) decreased directly after RCT, but increased above initial levels in the follow-up period 3 (t2) and 6 (t3) months after RCT. Patients with locoregional recurrence showed similar trends with respect to B, T cells and Tregs between t0 and t5. CD4+ T helper cells remained stably low between t0 and t5 in patients with locoregional recurrence compared to Ctrl. NKT/NK cell subsets (CD56+/CD69+, CD3-/CD56+, CD3-/CD94+, CD3-/NKG2D+, CD3-/NKp30+, CD3-/NKp46+) increased continuously up to 6 months after RCT (t0-t3) in patients without locoregional recurrence, whereas in patients with locoregional recurrence, these subsets remained stably low until time of recurrence (t5).Conclusion: Monitoring the kinetics of lymphocyte subpopulations especially activatory NK cells before and after RCT might provide a clue with respect to the development of an early locoregional recurrence in patients with SCCHN. However, studies with larger patient cohorts are needed.

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Section: Discussionsupporting

confidence: 85%

Comparison of the Composition of Lymphocyte Subpopulations in Non-relapse and Relapse Patients With Squamous Cell Carcinoma of the Head and Neck Before, During Radiochemotherapy and in the Follow-up Period: a Multicenter Prospective Study of the German Cancer Consortium Radiation Oncology Group (DKTK-ROG)

Niu

Combs

Linge

et al. 2020

Preprint

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“…Moreover, regarding the KB16 cell line that was used and derived from KB cells, it contains the E1A and E1B regions of the Ad2 virus and was chosen for this study as an alternate to analyzing PBMC cytokine secretion in the presence of viral particles. 9,10 The results of this study indicate that PBMCs obtained from healthy donors that were incubated with conditioned medium from SCC cell lines did not secrete IFN-γ. In this experimental model, the PBMCs were not activated by dendritic cells, which may decline their function; however the secretion of IFN-γ was expected to some degree since the cells from control groups were able to produce this cytokine when stimulated by PHA.…”

Section: Discussionmentioning

confidence: 78%

“…However, neither the nature of the regulatory factors present, nor the steps involved in the resistance of transformed oral keratinocytes, has been fully elucidated. 9,10 Correspondingly, the present study was designed to analyze the early response of healthy donor PBMCs versus PBMCs directly exposed to tumor-derived products. Moreover, regarding the KB16 cell line that was used and derived from KB cells, it contains the E1A and E1B regions of the Ad2 virus and was chosen for this study as an alternate to analyzing PBMC cytokine secretion in the presence of viral particles.…”

Section: Discussionmentioning

confidence: 99%

Response of peripheral blood mononuclear cells to conditioned medium from cultured oral squamous cell carcinomas

França

Barros²,

Lotufo

et al. 2011

Braz. oral res.

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Response of peripheral blood mononuclear cells to conditioned medium from cultured oral squamous cell carcinomas Abstract: The current study investigated the capacity for tumor factors secreted by head and neck squamous cell carcinoma (HNSCC) cell lines, KB, KB16, and HEP, to induce the secretion of various cytokines from peripheral blood mononuclear cells (PBMCs). PBMCs were isolated from blood samples collected from six healthy volunteers and these cells were incubated for 6, 24, 48, or 72 hours in the presence of 50% conditioned medium collected from cultured cell lines pretreated with, or without, stimulants such as phytohemagglutinin (PHA) or lipopolysaccharides (LPS). Aliquots of each supernatant were then assayed for levels of IFN-γ, vascular endothelial growth factor (VEGF), TNF-α, and IL-4 using enzyme linked immunosorbent assays (ELISAs). Data collected were analyzed using Student's t-test, an ANOVA test followed by Tukey's test, and tests of Pearson's Correlation. PBMCs cultured with KB16-conditioned medium produced the highest levels of IFN-γ. VEGF was also detected in conditioned media collected from all of the squamous cell carcinoma (SCC) cell lines used, and a significant difference in VEGF levels between control and KB-or KB16-conditioned media was observed. TNF-α was secreted by all PBMC groups within 6 hours of receiving conditioned media, and these levels increased up to the 24 hour timepoint, after which levels of TNF-α stabilized. In contrast, none of the supernatant samples contained detectable levels of IL-4. In combination, these data suggest that direct contact between fresh human PBMCs and conditioned media from tumor cells induces the secretion of TNF-α and VEGF by PBMCs, and this represents an initial angiogenic response.

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“…Activating NK cells have the ability to directly kill certain target cells. Many studies have demonstrated that NK cell subset distribution and the change in the expression of early activation antigen, CD3 − /CD56 + /CD69 + NK cells with respect to clinical response [ 5 , 28 – 30 ]. Although the activating NK cells have been established to have the ability to attack the target cells, we still have to actually carry out the activity assay of killing target cells for understanding NK cell activity.…”

Section: Discussionmentioning

confidence: 99%

Health effects of a forest environment on natural killer cells in humans: an observational pilot study

et al. 2018

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Health effect assessments based on natural killer (NK) cells are an important emerging area of human health. We recruited 90 forest staff members in Xitou, Taiwan and 110 urban staff members in Taipei to investigate the health effects of forest environment exposure on NK cells (CD3−/CD56+) and activating NK cells (CD3−/CD56+/CD69+) in humans. We also invited 11 middle-aged volunteers in a pilot study to participate in a five-day/four-night forest trip to Xitou forest to investigate the health effects of a forest trip on NK cells and activating NK cells. Results showed that NK cells were higher in the forest group (19.5 ± 9.1%) than in the urban group (16.4 ± 8.4%). In particular, the percentage of NK cells was significantly higher in the forest group than in the urban group among the subgroups of male, a higher body mass index (≥ 25 kg/m2), without hypertension, lower high-sensitivity C-reactive protein, hyperglycemia, without smoking habit, and with tea drinking habit. After the five-day trip in Xitou forest, the percentage of activating NK cells of the invited participants from Taipei increased significantly after the trip to Xitou forest (0.83 ± 0.39% vs. 1.72 ± 0.1%). The percentage of activating NK cells was 1.13 ± 0.43%, which was higher than the baseline value of 0.77 ± 0.38% before the forest trip among the seven subjects who participated in the follow-up study four days after returning to Taipei. This study suggests that exposure to forest environments might enhance the immune response of NK cells and activating NK cells in humans.

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Changes in peripheral blood lymphocyte phenotypes distribution in patients with oral cancer/oral leukoplakia in Taiwan

Cited by 13 publications

References 41 publications

Response of peripheral blood mononuclear cells to conditioned medium from cultured oral squamous cell carcinomas

Health effects of a forest environment on natural killer cells in humans: an observational pilot study

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