Changes in pathophysiology of tumor growth and functional activity of the hypothalamic-pituitary-thyroid axis in rats of both sexes with the development of Guerin's carcinoma on the background of hypothyroidism

Frantsiyants, Elena M.; Бандовкина, В. А.; Kaplieva, Irina V.; Surikova, Ekaterina I.; Pogorelova, Yulia A.; Pogorelova, Yu. A.; Trepitaki, Lidia K.; Cheryarina, Natalia D.; Nemashkalova, Ludmila A.; Arakelova, A.Y.

doi:10.37748/2686-9039-2022-3-4-3

Cited by 7 publications

(4 citation statements)

References 22 publications

(22 reference statements)

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“…Previously, we found that induced hypothyroidism inhibited the growth of transplanted Guerin's carcinoma and increased life expectancy only in female rats, while in male rats, despite the fact that the size of the tumors was smaller at the initial stages, after 2 weeks of the experiment it was comparable with the parameters in the animals with the independent tumor growth, and no increase in life expectancy was observed [16,17]. An analysis of the content of hypothalamic Gn-releasing and pituitary tropic hormones LH, FSH and prolactin (PRL) in rats of both sexes is presented in Table 1 herein.…”

Section: Resultsmentioning

confidence: 84%

Disorders in connections of the hypothalamic-pituitarygonadal axis in rats with Guerin’s carcinoma against the background of induced hypothyroidism

Бандовкина¹,

Frantsiyants²,

Kaplieva³

et al. 2023

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Aims: to study the content of regulatory peptides in the hypothalamus and pituitary gland, as well as sex steroids in the gonads in rats of both sexes with Guerin’s carcinoma against the background of hypothyroidism. Materials and methods. Our experiment was performed in white outbred rats of both sexes with induced hypothyroidism, against the background of which Guerin’s carcinoma was transplanted. In 1% homogenates of the pituitary and the hypothalamus and in 10% homogenates of the gonads in the animals from the main and reference groups, the levels of GnRH (Casabio, China) were determined by the ELISA method using standard kits, and the content of LH, FSH, estradiol (E2), testosterone (T) and progesterone (P4) by the RIA method (Immunotech, Czech Republic). Statistical analysis of the results was performed using the Statistica 10.0 software package. Results. In females of the main group, the level of GnRH in the hypothalamus increased by 1.6 times, but the content of LH and FSH in the pituitary gland decreased, while in males the content of GnRH decreased by 1.4 times and FSH by 1.6 times compared with the intact animals. In the gonads of females of the main group, the content of E2 and T decreased by 1.3 times, but the level of P4 increased by 4.7 times, while in males the T production decreased by 7.1 times. Hypothyroidism, against the background of which Guerin’s carcinoma developed, had a significant effect both on the central links of the HPG axis and the peripheral ones, producing an additional hormonal imbalance in sex steroids

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Section: Resultsmentioning

confidence: 84%

Disorders in connections of the hypothalamic-pituitarygonadal axis in rats with Guerin’s carcinoma against the background of induced hypothyroidism

Бандовкина¹,

Frantsiyants²,

Kaplieva³

et al. 2023

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“…Previously, we found that in female rats, hypothyroidism slowed down the increase in the volume of primary tumors of Guerin's carcinoma and prolonged their life expectancy. In males, a slight slowdown in the tumor growth was noted only up to day 14 after inoculation, then the tumor grew rapidly, and its volume did not differ from that recorded in the reference group, and no increase in life expectancy was reported [9,10]. It was just during that period, upon expiration of 19 days of the tumor growth, when significant gender differences in the growth of Guerin's carcinoma were revealed by us, and studies of indicators in blood and tissues were carried out.…”

Section: Resultsmentioning

confidence: 93%

Influence by hypothyroidism on the level of sex hormones in blood and their intracrine content in the tumor in rats with Guerin’s carcinoma

Bandovkina¹,

Frantsiyants²,

Kaplieva³

et al. 2023

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The aim of our research work was to study the level of sex steroids in blood, the tumor and the perifocal zone in rats of both sexes with Guerin’s carcinoma against the background of hypothyroidism. Materials and methods. The experiment was performed in 110 outbred rats of both sexes. Hypothyroidism was induced in animals for 30 days with Mercazolil medication, and then Guerin’s carcinoma was transplanted (the main group). The reference groups included animals with an independent growth of Guerin’s carcinoma and with independent hypothyroidism, as well as intact animals (the norm). On the 18th day of the tumor growth, the animals were sacrificed, and, using standard RIA kits, in the serum, the tumor homogenates and the perifocal zone, the levels of estradiol (E2), testosterone (T) and progesterone (P4) were determined. Results. In comparison with intact animals, hypothyroidism caused an increase in the blood content of E2 in animals of both sexes by 2.2-2.4 times and T by 1.4-16 times, and P4 by 1.7 times only in females, but recorded was a decrease therein in males by 2.4 times. The growth of Guerin’s carcinoma resulted in a 2.5-5.5-fold decrease in E2 in blood of the animals of both sexes, an increase in T by 2.1 times and P4 by 3 times in the females, but a decrease in T by 2.6 times without changing P4 in the males. In the main group, in the animals of both sexes, similarly to the processes in hypothyroidism, the level of E2 and P4 increased by 1.4-1.6 times, and in females also T by 4.4 times, compared with the intact animals. Conclusion. Hypothyroidism and the growth of Guerin’s carcinoma changed E2 in different directions in animals of both sexes the level of sex hormones in blood and shifted the steroid balance in the tumor and its perifocal zone. In the females of the main group, the saturation of the tumor with estrogens, androgens and progesterone decreased, while in the males, on the contrary, the concentration of steroids increased.

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“…It is known that the growth of a malignant tumor has a significant and often sex-dependent effect on the body's regulatory axes [9]. Moreover we previously found that induced hypothyroidism had an inhibitory effect on the growth of Guerin's carcinoma and S45 sarcoma only in the females, but not in the males [10,11].…”

Section: Introductionmentioning

confidence: 79%

Changes in the functional activity of the hypothalamic-pituitaryadrenal axis in rats of both sexes with Guerin’s carcinoma against the background of hypothyroidism

Kit,

Frantsiyants,

Bandovkina

et al. 2023

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The hypothalamic-pituitary-adrenal axis (HPA) makes the body’s response to various stress factors, interacting with the thyroid and gonadal axis. The aim of our work was to study the effect by induced hypothyroidism on changes in the functioning of the HPA axis in rats with Guerin’s carcinoma of both sexes. Materials and methods. The experiment was performed in white outbred rats of both sexes. Hypothyroidism was induced in animals by medication for 30 days with Mercazolil, and then Guerin’s carcinoma was transplanted (the main group). The reference groups covered animals with the independent growth of Guerin’s carcinoma and with independent hypothyroidism, as well as intact animals (the normal conditions). On day 18 of the tumor growth, the animals were sacrificed, and, using standard ELISA kits, in the serum and the homogenates of the adrenal glands, the tumor and the perifocal zone, the level of cortisol was determined; in the homogenates of the hypothalamus we measured the concentration of CT-releasing hormone (CRH) and in the pituitary gland the concentration of ACTH. Results. Induced hypothyroidism both in males and females caused adrenal insufficiency. The growth of Guerin’s carcinoma increased the level of CT-releasing hormone in males by 9.6 times and cortisol in the adrenal glands in females and males by 1.3 times and 2.3 times (p<0.05), respectively. In the main group females and males we revealed a decrease in the level of CT-releasing hormone of the hypothalamus by an average of 1.4 times, ACTH in the pituitary gland by 1.5-1.8 times, but an increase in the cortisol concentration in blood and the adrenal glands only in males by an average of 1.5 times (p˂0.05). Only in males of the main group in the tumor and its perifocal zone, the content of cortisol increased by 5.9 times and 1.6 times (р˂0.05), respectively. Conclusion. The HPA axis has a gender-specific response to the growth of a malignant tumor, both in its independent growth variant and against the background of hypothyroidism, which in its independent variant causes adrenal insufficiency.

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Changes in pathophysiology of tumor growth and functional activity of the hypothalamic-pituitary-thyroid axis in rats of both sexes with the development of Guerin's carcinoma on the background of hypothyroidism

Cited by 7 publications

References 22 publications

Disorders in connections of the hypothalamic-pituitarygonadal axis in rats with Guerin’s carcinoma against the background of induced hypothyroidism

Disorders in connections of the hypothalamic-pituitarygonadal axis in rats with Guerin’s carcinoma against the background of induced hypothyroidism

Influence by hypothyroidism on the level of sex hormones in blood and their intracrine content in the tumor in rats with Guerin’s carcinoma

Changes in the functional activity of the hypothalamic-pituitaryadrenal axis in rats of both sexes with Guerin’s carcinoma against the background of hypothyroidism

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