Changes in Oxidative Stress Parameters and Antioxidant Status in Lung Cancer: Western Blot Analysis of Nitrotyrosine and Protein Carbonyls Content

Korkmaz, Gülcan Güntaş; Inal, B. B.; Ortakoylu, Gonenc; Irmak, Hulya; Kara, Ateş; Gelışgen, Remise; Ogurlu, Ozcan; Uzun, Hafize

doi:10.7754/clin.lab.2013.130407

Cited by 6 publications

(5 citation statements)

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“…Increasing the number of participants will, of course, improve the confidence of the generated data. However, the low p -values of our data and the fact that our main findings, which show that PBMCs from lung cancer patients are characterized by aberrations in the GSH/GSSG ratio, DNA repair mechanisms and the apoptosis rates, corroborate previous studies, are strengthening the notion that lung cancer is associated with oxidative stress and a deregulated DDR network [ 6 , 17 , 18 , 19 , 22 , 25 , 26 , 27 ].…”

Section: Discussionsupporting

confidence: 91%

“…Moreover, antioxidative biomarkers, such as superoxide dismutase, glutathione peroxidase and vitamin C were lower in the blood of lung cancer patients than in HC [ 17 ]. Oxidative-induced posttranslational protein modifications are also increased in the plasma of lung cancer patients [ 18 ]. In addition, lung cancer patients have high urinary levels of 8-oxo-2′-deoxyguanosine and isoprostanes that are produced in vivo by free radical-catalyzed peroxidation of arachidonic acid [ 17 , 19 ].…”

Section: Discussionmentioning

confidence: 99%

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Oxidative Stress and Deregulated DNA Damage Response Network in Lung Cancer Patients

et al. 2022

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The deregulated DNA damage response (DDR) network is associated with the onset and progression of cancer. Herein, we searched for DDR defects in peripheral blood mononuclear cells (PBMCs) from lung cancer patients, and we evaluated factors leading to the augmented formation of DNA damage and/or its delayed/decreased removal. In PBMCs from 20 lung cancer patients at diagnosis and 20 healthy controls (HC), we analyzed oxidative stress and DDR-related parameters, including critical DNA repair mechanisms and apoptosis rates. Cancer patients showed higher levels of endogenous DNA damage than HC (p < 0.001), indicating accumulation of DNA damage in the absence of known exogenous genotoxic insults. Higher levels of oxidative stress and apurinic/apyrimidinic sites were observed in patients rather than HC (all p < 0.001), suggesting that increased endogenous DNA damage may emerge, at least in part, from these intracellular factors. Lower nucleotide excision repair and double-strand break repair capacities were found in patients rather than HC (all p < 0.001), suggesting that the accumulation of DNA damage can also be mediated by defective DNA repair mechanisms. Interestingly, reduced apoptosis rates were obtained in cancer patients compared with HC (p < 0.001). Consequently, the expression of critical DDR-associated genes was found deregulated in cancer patients. Together, oxidative stress and DDR-related aberrations contribute to the accumulation of endogenous DNA damage in PBMCs from lung cancer patients and can potentially be exploited as novel therapeutic targets and non-invasive biomarkers.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Oxidative Stress and Deregulated DNA Damage Response Network in Lung Cancer Patients

et al. 2022

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“…The previous study has shown a significant difference in advanced oxidation protein product levels between patients with lung cancer and healthy controls. [28,29] However, the methods used in such studies were different from those in the present study.…”

Section: Discussionmentioning

confidence: 78%

Sera total oxidant/antioxidant status in lung cancer patients

Xiang¹,

Feng

Geng

et al. 2019

Medicine

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We investigated oxidative stress parameters in the sera of patients with lung cancer and healthy individuals to evaluate their correlations with lung cancer.Ninety-four lung cancer patients and 64 healthy controls were enrolled after obtaining informed consent. Their sera oxidative stress parameters were measured.Total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) were significantly different between patients and healthy groups (all P < .001). TAS gradually decreased and TOS and OSI gradually increased from stage I to III, but it did not reach statistical significance (all P > .05). TAS and OSI were significantly different between the nonsmoking and smoking groups, radiotherapy and without radiotherapy groups, chemotherapy and without chemotherapy groups (P < .05), but not TOS (P > .05). In a receiver operating characteristic curve analysis comparing patients with lung cancer with healthy controls, the Youden indices of TOS, TAS, and OSI were 0.541, 0.532, and 1, respectively.The oxidative stress may be correlation with lung cancer staging. Smoking, surgery, radiotherapy, and chemotherapy showed correlation with parts oxidative stress parameters.

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“…Advanced oxidation protein products (AOPP) are elevated in several chronic inflammatory diseases with an important overload of oxidative stress [ 21 ]. In lung pathologies, high plasma levels of AOPP have been found [ 22 , 23 ], suggesting that serum AOPP levels could play a critical role as a biomarker for pulmonary diseases.…”

Section: Introductionmentioning

confidence: 99%

Serum Biomarkers in Differential Diagnosis of Idiopathic Pulmonary Fibrosis and Connective Tissue Disease-Associated Interstitial Lung Disease

César

Lopez-Lopez

Lara

et al. 2021

JCM

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Introduction: The goal of this study is to determine whether Advanced glycosylated end-products (AGE), Advanced oxidation protein products (AOPP) and Matrix metalloproteinase 7 (MMP7) could be used as differential biomarkers for idiopathic pulmonary fibrosis (IPF) and connective tissue disease-associated interstitial lung disease (CTD-ILD). Method: Seventy-three patients were enrolled: 29 with IPF, 14 with CTD-ILD, and 30 healthy controls. The study included a single visit by participants. A blood sample was drawn and serum was analysed for AGE using spectrofluorimetry, AOPP by spectrophotometry, and MMP7 using sandwich-type enzyme-linked immunosorbent assay. Results: AGE, AOPP and MMP7 serum levels were significantly higher in both IPF and CTD-ILD patients versus healthy controls; and AGE was also significantly elevated in CTD-ILD compared to the IPF group. AGE plasma levels clearly distinguished CTD-ILD patients from healthy participants (AUC = 0.95; 95% IC 0.86–1), whereas in IPF patients, the distinction was moderate (AUC = 0.78; 95% IC 0.60–0.97). Conclusion: In summary, our results provide support for the potential value of serum AGE, AOPP and MMP7 concentrations as diagnostic biomarkers in IPF and CTD-ILD to differentiate between ILD patients and healthy controls. Furthermore, this study provides evidence, for the first time, for the possible use of AGE as a differential diagnostic biomarker to distinguish between IPF and CTD-ILD. The value of these biomarkers as additional tools in a multidisciplinary approach to IPF and CTD-ILD diagnosis needs to be considered and further explored. Multicentre studies are necessary to understand the role of AGE in differential diagnosis.

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Changes in Oxidative Stress Parameters and Antioxidant Status in Lung Cancer: Western Blot Analysis of Nitrotyrosine and Protein Carbonyls Content

Cited by 6 publications

References 0 publications

Oxidative Stress and Deregulated DNA Damage Response Network in Lung Cancer Patients

Oxidative Stress and Deregulated DNA Damage Response Network in Lung Cancer Patients

Sera total oxidant/antioxidant status in lung cancer patients

Serum Biomarkers in Differential Diagnosis of Idiopathic Pulmonary Fibrosis and Connective Tissue Disease-Associated Interstitial Lung Disease

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