Changes in oxidative stress biomarkers in Sinonovacula constricta in response to toxic metal accumulation during growth in an aquaculture farm

Chen, Mengxu; Zhou, Jiayi; Lin, Jinhuang; Tang, Hongchao; Shan, Yuxi; Chang, Alan K.; Xiao, Ying

doi:10.1016/j.chemosphere.2020.125974

Cited by 24 publications

(14 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, aquatic foods are regarded as ideal sources of quality protein and essential fatty acids, which could improve a range of human pathologies [ 2 ]. Over the past few decades, Chinese aquaculture production has been steadily pursuing growth, and it has become the fastest growing food production sector [ 3 ]; however, during the farming process, oxidative stress damage is ubiquitous, and it often seriously affects the growth performance, stress tolerance, and pathogen sensitivity of aquatic animals [ 4 , 5 , 6 ].…”

Section: Introductionmentioning

confidence: 99%

Oxidative Stress Can Be Attenuated by 4-PBA Caused by High-Fat or Ammonia Nitrogen in Cultured Spotted Seabass: The Mechanism Is Related to Endoplasmic Reticulum Stress

Dong

Xia

et al. 2022

Antioxidants

View full text Add to dashboard Cite

Oxidative stress is a common phenomenon in aquaculture, which can be induced by nutritional or environmental factors. Generally, oxidative stress causes poor growth performance, metabolic dysregulation, and even the death of aquatic animals. To identify a nutritional intervention strategy, high-fat diet (HFD) feeding (Experiment I) and acute ammonia nitrogen challenge (Experiment II) tests were carried out. In Experiment I, HFD feeding significantly decreased the growth performance concomitantly with excessive fat deposition in the liver and abdomen. The addition of 4-PBA in the diet improved the excessive fat accumulation. The activities of antioxidative enzymes were suppressed, and the levels of lipid and protein peroxidation were increased, indicating that HFD feeding induced oxidative stress. The endoplasmic reticulum stress (ERs) related genes were downregulated in the HFD group. Under a transmission electron microscope (TEM), more swollen and dilated ER lumen could be observed. These results indicated that the HFD induced ERs activation. Although 4-PBA acted as a potent ERs inhibitor, as evidenced by the alleviated alterations of ERs molecules and the ER ultrastructure, the oxidative stress was also attenuated by 4-PBA. In Experiment II, dietary 4-PBA improved the tolerance to the acute ammonia nitrogen challenge, as lower mortality and serum aminotransferase activity was found. Further results showed that 4-PBA decreased the peroxidation content and attenuated ERs, thus confirming the correlation between oxidative stress and ERs. Our findings showed that dietary 4-PBA supplementation can attenuate oxidative stress induced by a HFD or acute ammonia challenge; the mechanism is related to its potent inhibition effect for ERs.

show abstract

Section: Introductionmentioning

confidence: 99%

Oxidative Stress Can Be Attenuated by 4-PBA Caused by High-Fat or Ammonia Nitrogen in Cultured Spotted Seabass: The Mechanism Is Related to Endoplasmic Reticulum Stress

Dong

Xia

et al. 2022

Antioxidants

View full text Add to dashboard Cite

show abstract

“…In recent years, pollution caused by toxic metals has become a serious environmental problem that continues to increase in magnitude as a result of rapid industrialization and the massive use of fertilizers (Chen et al, 2020;Zhang and Gao, 2015). Among the toxic metals of concern is cadmium (Cd), which is widely distributed in aquatic ecosystems.…”

Section: Introductionmentioning

confidence: 99%

“…Among the toxic metals of concern is cadmium (Cd), which is widely distributed in aquatic ecosystems. Even at low concentrations, Cd is highly toxic to aquatic organisms because it is non-biodegradable and it has a long biological half-life (Capriello et al, 2019;Park et al, 2020), and therefore, it can accumulate in the food chain, causing tissue injury and threatening the health of aquatic animals (Chen et al, 2020;Jing et al, 2019;Pan et al, 2018). At the cellular level, Cd also has the potential to affect cellular antioxidant defenses and damage the DNA repair systems, indicating that Cd plays a vital role in apoptosis (Lin et al, 2017;Park et al, 2020;Xia et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Mitochondria are the key intracellular targets for Cd stress (Bhansali et al, 2017), and Cd exposure can damage the integrity of the mitochondria in aquatic organisms, both structurally and functionally (Adiele et al, 2011) Through the electron transport chain of the mitochondria, O 2 •− generates H 2 O 2 , which is then split by catalase into water and oxygen or is partially reduced to hydroxide radical (•OH) in a Fenton reaction, causing a wider cellular oxidative damage (Feng et al, 2014) Bivalves are widely distributed in the water ecosystem. As these animals are lter feeders with poor locomotion ability, they are rather sensitive to the contamination of the aquatic environment by toxic metals, making them a group of important animals for aquatic toxicology research and for the evaluation of pollution by toxic metals (Chandurvelan et al, 2015;Chen et al, 2020;Ji et al, 2019). The clam Meretrix meretrix is one of the important cultivated conventional marine bivalves in China, because of its economic and ecological importance.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cadmium-induced oxidative stress in Meretrix meretrix gills leads to mitochondria-mediated apoptosis

et al. 2021

Self Cite

View full text Add to dashboard Cite

Cadmium (Cd) is one of the most important marine environmental pollutants that can cause oxidative damage and apoptosis in living organisms, and mitochondria are the key target cell organelles of Cd toxicity. In this study, we investigated the effect of Cd on the mitochondria of Meretrix meretrix gill cells and the underlying mechanism of mitochondria-mediated apoptosis following exposure to the metal.Exposure of the clams to 1.5, 3, 6 and 12 mg L −1 Cd 2+ solutions led to swollen mitochondria compared with the untreated clams. The mitochondria also became vacuolated at the higher Cd 2+ concentrations.Biochemical assays showed that monoamine oxidase (MAO) activity and mitochondrial membrane potential (Δψm) increased at 1.5 mg L −1 Cd 2+ , but decreased at higher Cd 2+ concentrations, while the activities of malate dehydrogenase (MDH) and cytochrome oxidase (CCO) and the scavenging capacities of anti-superoxide anion (ASA) and anti-hydroxy radical (AHR) all decreased with increasing Cd 2+ concentrations. Signi cant increases in the levels of MDA and H 2 O 2 as well as in the activity levels of caspase-3, -8, and -9 were also observed in the Cd 2+ -treated clams. The results implied that Cd might induce apoptosis in M. meretrix via the mitochondrial caspase-dependent pathway.

show abstract

“…The activity of SOD is indispensable for intracellular perishing of cell-damaging peroxide species and the inhibition of cytotoxicity caused by ROS overproduction. , Based on this, SOD activity was assessed in this study. Compared to the IR-model group, as illustrated in Table , the activity of SOD was significantly increased by the novel oligopeptides ( P < 0.05).…”

Section: Resultsmentioning

confidence: 99%

Assessment of Novel Oligopeptides from Rapeseed Napin (Brassica napus) in Protecting HepG2 Cells from Insulin Resistance and Oxidative Stress

Yao

et al. 2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

Oligopeptides (Thr–His–Leu–Pro–Lys (THLPK), His–Pro–Leu–Lys (HPLK), Leu–Pro–Lys (LPK), His–Leu–Lys (HLK), and Leu–His–Lys (LHK)) are newly identified from rapeseed napin (Brassica napus) protein-derived hydrolysates with the capability of upregulating glucose transporter-4 (GLUT4) expression and translocation. However, whether each of them enhances GLUT4 expression and translocation and their specific mechanisms remain unclear. Here, we assess the effects of the oligopeptides against insulin resistance (IR) and oxidative stress in hepatocytes and screen out the most antidiabetic one. Specifically, compared with other oligopeptides, LPK not only remarkably elevated glucose consumption to 8.45 mmol/L protein; superoxide dismutase (SOD) activity to 319 U/mg protein; GLUT4 expression and translocation; and phosphorylated level of insulin receptor substrate-1 (IRS-1), phosphatidylinositol 3-kinase (PI3K), and protein kinase B (Akt) (P < 0.05) but also remarkably attenuated the reactive oxygen species (ROS) level to 2255, lactate dehydrogenase (LDH) activity to 20.5 U/mg protein, malondialdehyde (MDA) content to 241 nmol/mg protein, and NO content to 1302 μmol/mL protein (P < 0.05). These findings demonstrated that antidiabetic oligopeptide LPK possessed the most potential to protect HepG2 cells from IR and oxidative stress via activating IRS-1/PI3K/Akt/GLUT4 and regulating common oxidative markers in vitro.

show abstract

Changes in oxidative stress biomarkers in Sinonovacula constricta in response to toxic metal accumulation during growth in an aquaculture farm

Cited by 24 publications

References 32 publications

Oxidative Stress Can Be Attenuated by 4-PBA Caused by High-Fat or Ammonia Nitrogen in Cultured Spotted Seabass: The Mechanism Is Related to Endoplasmic Reticulum Stress

Oxidative Stress Can Be Attenuated by 4-PBA Caused by High-Fat or Ammonia Nitrogen in Cultured Spotted Seabass: The Mechanism Is Related to Endoplasmic Reticulum Stress

Cadmium-induced oxidative stress in Meretrix meretrix gills leads to mitochondria-mediated apoptosis

Assessment of Novel Oligopeptides from Rapeseed Napin (Brassica napus) in Protecting HepG2 Cells from Insulin Resistance and Oxidative Stress

Contact Info

Product

Resources

About