Changes in oxidative enzyme activity during interspecific mycelial interactions involving the white-rot fungus Trametes versicolor

Hiscox, Jennifer; Baldrián, Petr; Rogers, Hilary J.; Boddy, Lynne

doi:10.1016/j.fgb.2010.03.007

Cited by 98 publications

(94 citation statements)

References 53 publications

(71 reference statements)

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“…Changes in secondary metabolism appear to be fundamental to IS in Heterobasidion, which is consistent with previous observations for heterospecific interactions (e.g., Eyre et al, 2010;Hiscox et al, 2010). This is evident in that certain DE genes encoded proteins (e.g., farnesyldiphosphate synthase) involved in secondary metabolite production (e.g., terpenoids) (Liu et al, 1999).…”

Section: The Is Response Involves a Balance Between Toxin Production supporting

confidence: 90%

Gene expression associated with intersterility in Heterobasidion

Nest¹,

Olson²,

Karlsson³

et al. 2014

Fungal Genetics and Biology

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Intersterility (IS) is thought to prevent mating compatibility between homokaryons that belong to different species. Although IS in Heterobasidion is regulated by the genes located at the IS loci, it is not yet known how the IS genes influence sexual compatibility and heterokaryon formation. To increase our understanding of the molecular events underlying IS, we studied mRNA abundance changes during IS compatible and incompatible interactions over time. The clustering of the transcripts into expression profiles, followed by the application of Gene Ontology (GO) enrichment pathway analysis of each of the clusters, allowed inference of biological processes participating in IS. These analyses identified events involved in mating and sexual development (i.e., linked with IS compatibility), which included processes associated with cell-cell adhesion and recognition, cell cycle control and signal transduction.We also identified events potentially involved in overriding mating between individuals belonging to different species (i.e., linked with IS incompatibility), which included reactive oxygen species (ROS) production, responses to stress (especially to oxidative stress), signal transduction and metabolic biosynthesis. Our findings thus enabled detection and characterization of gene expression changes associated with IS in Heterobasidion, as well as identification of important processes and pathways associated with this phenomenon. Overall, 1 the results of this study increase current knowledge regarding the molecular mechanisms underpinning IS in Heterobasidion and allowed for the establishment of a vital baseline for further studies.

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Section: The Is Response Involves a Balance Between Toxin Production supporting

confidence: 90%

Gene expression associated with intersterility in Heterobasidion

Nest¹,

Olson²,

Karlsson³

et al. 2014

Fungal Genetics and Biology

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“…These multicopper oxidases are known to be strongly overproduced by white-rot fungi during interactions (1,10,12). Closer analysis of the RNA-Seq data revealed that four transcripts encoded putative laccases, all of which were expressed at similarly high levels under all conditions by P. coccineus.…”

Section: Discussionmentioning

confidence: 99%

“…This reduction in biomass accumulation was coupled to the underexpression of a large number of transcripts encoding chitin synthases (a protein involved in cell wall synthesis), amino acid transporters, which are involved in nutrient uptake and cell wall recycling, and ATP-dependent RNA helicases, which are involved in translation and pre-mRNA splicing (56). Current knowledge also indicates that during an interaction, fungi produce and secrete enzymatic effectors, including metalloproteins (12,57) and secondary metabolites (1), in order to interfere with their competitors. Interacting fungi also have to repair the structures damaged during the interaction, such as cell walls (28) or DNA (11).…”

Section: Discussionmentioning

confidence: 99%

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Differential Gene Expression in Pycnoporus coccineus during Interspecific Mycelial Interactions with Different Competitors

Arfi

Levasseur

Record

2013

Appl Environ Microbiol

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Fungi compete against each other for environmental resources. These interspecific combative interactions encompass a wide range of mechanisms. In this study, we highlight the ability of the white-rot fungus Pycnoporus coccineus to quickly overgrow or replace a wide range of competitor fungi, including the gray-mold fungus Botrytis cinerea and the brown-rot fungus Coniophora puteana. To gain a better understanding of the mechanisms deployed by P. coccineus to compete against other fungi and to assess whether common pathways are used to interact with different competitors, differential gene expression in P. coccineus during cocultivation was assessed by transcriptome sequencing and confirmed by quantitative reverse transcription-PCR analysis of a set of 15 representative genes. Compared with the pure culture, 1,343 transcripts were differentially expressed in the interaction with C. puteana and 4,253 were differentially expressed in the interaction with B. cinerea, but only 197 transcripts were overexpressed in both interactions. Overall, the results suggest that a broad array of functions is necessary for P. coccineus to replace its competitors and that different responses are elicited by the two competitors, although a portion of the mechanism is common to both. However, the functions elicited by the expression of specific transcripts appear to converge toward a limited set of roles, including detoxification of secondary metabolites. Interspecific interactions are key elements in the ecology of fungi, which live in complex communities and often have to compete against each other for a territory and the resources it contains (1, 2). Competitive mycelial interactions usually result in replacement (one of the competitors overruns the other) or deadlock (neither fungus gains any territory). The outcome of these interactions will in turn affect the succession of species, community development, and substratum decay (3-5). This ability to eliminate competitors has also been used as a biotechnological tool, with the development of methods using aggressive, strongly antagonistic species, such as Trichoderma sp., to control plantpathogenic and wood rot fungi (1, 6, 7).When antagonistic relations occur, a broad array of responses can be observed, including changes in mycelial morphology at the interaction front, modification of metabolism, and production and secretion of extracellular enzymes (1,(8)(9)(10)(11). Two main pathways are used to gain an advantage over the competitor: development of attack or defense mechanisms and improvements in nutrient uptake (12). For instance, the overproduction of a large number of oxidative enzymes, such as laccases, phenoloxidases, and peroxidases, has been demonstrated in multiple interactions (13-16). As most of these enzymes are also secreted during oxidative and abiotic stresses, it has been suggested that they are part of a defensive function that contributes to the detoxification of reactive oxygen species (ROS). Laccases and peroxidases are also thought to be involved in morpholo...

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“…Recently, van der Wal et al (2015) demonstrated that the fungal community in old Q. robur stumps is similar to a randomly assembled community. Moreover, the status of development of each fungus and the status of interaction between the fungi will be relevant, too, since: (1) each fungus accomplishes an individual set and temporal pattern of extracellular oxidoreductase activities (Liers et al 2011), (2) these activities are higher when a fungus is capturing fresh unoccupied wood (Hahn et al 2013;Větrovský et al 2010), (3) during interaction of several fungal species, activities of Lacc or MnP can increase (Baldrian 2004;Hiscox et al 2010;Š najdr et al 2011), and (4) competition between fungi can hamper wood degradation (Fukasawa et al 2009b). This complex interplay of fungal species may be the reason for the observed extreme variability (Table 3) and cannot be explained by solely analyzing the fungal community.…”

Section: Lacc Genp and Mnp Activities Are Highly Variablementioning

confidence: 99%

Patterns of laccase and peroxidases in coarse woody debris of Fagus sylvatica, Picea abies and Pinus sylvestris and their relation to different wood parameters

et al. 2015

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Lignin and its degradation, particularly in forest ecosystems, play a major role in the global carbon cycle. Filamentous fungi equipped with extracellular oxidoreductases (oxidative enzymes), i.e., laccase, manganese-dependent peroxidases and several other peroxidases, are the key players in the bioconversion of lignin. In particular, for coarse woody debris (CWD), this process is poorly understood and the activities of laccase and peroxidases have never been studied on a large field scale. We investigated the activities of these enzymes in 701 samples of Fagus sylvatica, Picea abies and Pinus sylvestris CWD across three regions in Germany and analyzed their dependence on pH, water content, wood density, total lignin, organic extractives, metals, watersoluble lignin fragments and fungal species richness. Respective enzyme activities were present in 79 % of all samples, and the activities were highly variable and more frequent in F. sylvatica than in coniferous wood. Logistic regressions and correlations between enzyme activities and the variables revealed that the fungal community structure and the amount of water-soluble lignin fragments are most important determinants, and that the prevalent acidic pH in CWD is suitable to facilitate laccase and manganese peroxidase activities. Concentrations of metals (manganese, copper, iron) were sufficient to ensure synthesis and functioning of relevant enzymes. Based on this large field study, we conclude that laccase and peroxidases in CWD are highly relevant for lignin degradation, but the variable pattern of their secretion is the result of a complex array of wood parameters and the fungal community structure, which could only partly be resolved.

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Changes in oxidative enzyme activity during interspecific mycelial interactions involving the white-rot fungus Trametes versicolor

Cited by 98 publications

References 53 publications

Gene expression associated with intersterility in Heterobasidion

Gene expression associated with intersterility in Heterobasidion

Differential Gene Expression in Pycnoporus coccineus during Interspecific Mycelial Interactions with Different Competitors

Patterns of laccase and peroxidases in coarse woody debris of Fagus sylvatica, Picea abies and Pinus sylvestris and their relation to different wood parameters

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