Changes in Osmotic Fragility of Erythrocytes during Exercise in Athletic Horses.

Hanzawa, Kei; Watanabe, Shinji

doi:10.1294/jes.11.51

Cited by 23 publications

(26 citation statements)

References 59 publications

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“…This extent is dependent on cell volume, surface area, and functional integrity of cell membranes. Since it can be followed by changes in absorbance at 540 nm, the EOF test was frequently applied to the diagnosis of hemolytic diseases and oxidant alterations leading to destruction of erythrocytes [1,2,3]. For example, road transportation to slaughterhouses associated to heat stress had been demonstrated to cause increase of hemolysis in pig [3], goat [4] and camel [5].…”

Section: Introductionmentioning

confidence: 99%

Study of Incubation Conditions for Erythrocytes Osmotic Fragility Testing in Dromedary Camel (Camelus dromedarius)

Islah¹,

Bargaa²,

Youssef³

et al. 2016

International Journal of Research in Environmental Science

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Section: Introductionmentioning

confidence: 99%

Study of Incubation Conditions for Erythrocytes Osmotic Fragility Testing in Dromedary Camel (Camelus dromedarius)

Islah¹,

Bargaa²,

Youssef³

et al. 2016

International Journal of Research in Environmental Science

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“…The EOF is used to evaluate the adaptability of the erythrocytes, degree of hemolysis, diagnose hereditary anemia, and for screening in blood banks through the use of a single point bag [14,34].…”

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confidence: 99%

Physiological Responses of Erythrocytes of Goats to Transportation and the Mondulatory Role of Ascorbic Acid

Minka

Ayo

2010

The Journal of Veterinary Medical Science

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ABSTRACT. Experiments were performed with the aim of investigating the effect of road transportation for 12 hr on erythrocytes of goats during the hot-dry season and the modulatory role of ascorbic acid. Forty 2.5-3-year-old Red Sokoto goats weighing 23-25 kg and belonging to both sexes served as the subjects of the study. Twenty of the goats served as the experimental group and were administered ascorbic acid (AA) per os at a dosage rate of 100 mg/kg body weight; the other 20 served as controls and were given 10 ml each of sterile water. Forty minutes after the administration and loading, the goats were transported for 12 hr. EDTA blood samples collected before loading, after loading, immediately after transportation and subsequently on the 3rd and 7th days of post-transportation were used to determine the red blood cell (RBC) count, packed cell volume (PCV), hemoglobin (Hb), erythrocyte osmotic fragility (EOF), hematimetric (intrinsic) indices and hemoglobin index levels. The obtained results showed that handling, loading and transportation of the control goats induced significant (P<0.05) increases in RBC, Hb, EOF and hypochromic erythrocytes and a decrease (P<0.05) in the volume and average Hb content in RBCs. AA administration ameliorated all these changes. The present results suggest that road transportation for 12 hr during the hot-dry season could induce serious stress, resulting in hemolysis of erythrocytes, which was ameliorated by AA administration. In addition, the results demonstrated that EOF could be used as a diagnostic tool in road transportation stress.KEY WORDS: Ascorbic acid, erythrocytes, goats, hemolysis, transportation stress.J. Vet. Med. Sci. 72 (7): [875][876][877][878][879][880][881] 2010 Road transportation of livestock is a stress-inducing situation that may lead to psychological and physiological insult. Several studies have reported the responses of different physiological parameters to the effect of road transportation stress in other species of livestock [22,27,30]. In goats such studies are grossly inadequate [18,24]. Few reports have shown that goats are highly susceptibility to diseases following prolonged transportation by road under adverse climatic conditions [18]. The major stress factor affecting goats reared in regions with hot-climatic conditions and predominately raised under an extensive management system during road transportation are those of rounding-up, handling and loading and the adverse effect of high ambient temperature and relative humidity during the journey.Blood examinations are routinely used in diagnosis of the health status and evaluation of adaptability of livestock to stress factors [22,23]. One of the important blood parameters that may be measured during stress is erythrocytes. This is because erythrocytes are the chief cells that circulate oxygen in an organism and are highly susceptible to oxidative damage induced by free radicals or reactive oxygen species (ROS) generated under stressful conditions [20,39]. Studies in human subjects [39], rats ...

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“…The erythrocytes therefore, become more fluid and allow more movement and permeability of the cells, thereby damaging them. Furthermore, high temperatures recorded during transportation may cause proteins of the erythrocytes membrane to denature, which may lead to membrane disintegration and haemolysis [7,10,25]. Thirdly, transport-induced oxidative stress on erythrocytes due to the adverse effect of toxic ROS, presumably generated during the transportation, may also contribute to the deterioration of erythrocyte properties; leading to increase in haemolysis.This is true because the erythrocytes are susceptible to oxidative stress as a result of high contents of unsaturated fatty acids in their membranes [1].…”

Section: Discussionmentioning

confidence: 99%

“…The EOF may be employed as a biomarker and additional diagnostic tool for the assessment of transport-induce oxidative stress in quails. [7,10].The use of EOF as a biomarker of road transport-induced stress in livestock is a new field of study that may add to the arsenals of tests, currently used in the diagnosis of stress due to road transportation [11][12][13]. Body temperature measurement is an important method of evaluation of physiological status.…”

Section: Introductionmentioning

confidence: 99%

“…The ROS readily oxidise and damage essential biological molecules and increase the susceptibility of the body to diseases. The red blood cells (RBCs) are highly susceptible to oxidative stress as a result of high contents of oxygen and unsaturated fatty acids in their membranes [1,7]. The oxidation of RBCs has been used as a model system for the investigation of oxidative damage of biomembranes [8].…”

Section: Introductionmentioning

confidence: 99%

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Effects of Antioxidants Vitamin E and C on ErythrocyteFragility, HaemoglobinIndex and Colonic Temperature of Transported Japanese Quails (Coturnixcoturnix japonica)

Minka¹

2013

J Veterinar Sci Technolo

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The present study investigated the effects of antioxidants Vitamin E (VE) and C (ascorbic acid, AA) on erythrocytes osmotic fragility (EOF), haemoglobin index (HI), colonic temperature (CT) and recovery times of transported Japanese quails during the hot-dry conditions. Two hundred male Japanese quails were used. The birds were randomly and equally divided into four groups: VE and AA groups, administered with 0.5 mg/kg and 200 mg/ kg body weight of VE and AA, respectively, control transported group (control group), administered with 0.5 ml of sterile water, and sedentary non-transported group (S). One hour after the administration of the antioxidants, the birds were transported by road for 2 h. The EOF, HI and CT were recorded before transportation, immediately after transportation (0) and on 3 rd and 7 th day of post-transportation period. The results showed that transportation induced hyperthermia, increased EOF and hypochromic erythrocytes in the quails, the effects of which were mitigated by antioxidants VE and AA. Base-line values for EOF were returned to normal in the control, AA and VE quails on days 7, 3 and 0, respectively post-transportation. The findings showed that recovery time after transportation in quails should be 7 days; but the duration may be reduced to 3 days if treated with AA or eliminated completely if the quails are treated with VE before transportation. The ameliorating effect was more pronounced in quails administered with antioxidant VE. The EOF may be employed as a biomarker and additional diagnostic tool for the assessment of transport-induce oxidative stress in quails. [7,10].The use of EOF as a biomarker of road transport-induced stress in livestock is a new field of study that may add to the arsenals of tests, currently used in the diagnosis of stress due to road transportation [11][12][13]. Body temperature measurement is an important method of evaluation of physiological status. It is also an ideal indicator for the assessment of stress and adaptability of livestock, including poultry, to various environmental stress factors [14][15][16]. Effects of Antioxidants Vitamin E and C on ErythrocyteFragilitySeveral stress factors have been reported to decrease the level of body antioxidants, vitamin E (VE) and vitamin C, otherwise known as ascorbic acid (AA); resulting in increases in the body requirements in these vitamins [14,[17][18][19]. The administration of the vitamins exerts ameliorating effects which are well manifested; when the body AA and VE are either overwhelmed or exhausted as a result of increased ROS generation by stress factors that overtax the animal control systems [18,20]. Therefore, a decrease in the concentration of antioxidant vitamins causes an increase in lipid peroxidation in the plasma and tissues, leading to damage of cell membranes [21,22]. Previous reports have revealed the beneficial effects of supplementation of VE and AA on birds and animals, subjected to various oxidative stress factors [12,16,18,22].Several studies have evaluated the respon...

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Changes in Osmotic Fragility of Erythrocytes during Exercise in Athletic Horses.

Cited by 23 publications

References 59 publications

Study of Incubation Conditions for Erythrocytes Osmotic Fragility Testing in Dromedary Camel (Camelus dromedarius)

Study of Incubation Conditions for Erythrocytes Osmotic Fragility Testing in Dromedary Camel (Camelus dromedarius)

Physiological Responses of Erythrocytes of Goats to Transportation and the Mondulatory Role of Ascorbic Acid

Effects of Antioxidants Vitamin E and C on ErythrocyteFragility, HaemoglobinIndex and Colonic Temperature of Transported Japanese Quails (Coturnixcoturnix japonica)

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