Changes in nuclear protein pattern by glucocorticoid treatment of lymphoid cells

Bogert, Coby Van den; Bakker, Harry M.; Kuźela, S; Melis, Trudi E.; Kroon, A.M.

doi:10.1016/0022-4731(89)90246-x

Cited by 5 publications

(1 citation statement)

References 21 publications

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“…Whether these gene products actually represent the nuclease activity which mediates apoptosis remains controversial. Other investigations report that the lower molecular weight glucocorticoid-induced proteins actually represent histones that were preferentially released from the chromatin following endonucleolytic degradation of the thymocyte genome [34,48,49]. However, in a more recent study where radiolabelled 32p-DNA was employed as a DNA substrate in this gel assay, it was rather conclusively demonstrated that the lower molecular weight nuclease activity was unrelated to histone proteins [501.…”

Section: Detection Of Apoptotic Endonuclease Activity Using An Exogenmentioning

confidence: 99%

A biochemical hallmark of apoptosis: Internucleosomal degradation of the genome

1992

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Apoptosis, or programmed cell death, is an endogenous cellular process whereby an external signal activates a metabolic pathway that results in cell death. This form of cell death appears to be a common feature in many biological processes where cell deletion is a mechanism for altering tissue structure and function. Historically, apoptosis has been studied using histological techniques; however, more recent interest has focused on analyzing this process at the biochemical level. A biochemical hallmark of apoptosis is a characteristic form of DNA degradation in which the genome is cleaved at internucleosomal sites, generating a 'ladder' of DNA fragments when analyzed by agarose gel electrophoresis. A number of assay systems have been developed to study this nuclease activity. For example, nuclease activity has been analyzed by measuring the release of endogenous DNA from apoptotic cells, by flow cytometric analysis of apoptotic cells and by analyzing in situ apoptotic nuclease activity in polyacrylamide gels containing DNA. Use of these assay systems has enabled investigators to study the signal transduction pathways that mediate apoptosis and to characterize the endonuclease itself. Future biochemical studies in this field will focus on isolating the genes and gene products that mediate apoptosis.

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Section: Detection Of Apoptotic Endonuclease Activity Using An Exogenmentioning

confidence: 99%

A biochemical hallmark of apoptosis: Internucleosomal degradation of the genome

1992

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Identification, purification, and characterization of a calcium-dependent endonuclease (NUC18) from apoptotic rat thymocytes. NUC18 is not histone H2B.

Gaido

Cidlowski

1991

Journal of Biological Chemistry

278

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Apoptosis: mode of cell death induced in T cell leukemia lines by dexamethasone and other agents

1991

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Glucocorticoids can mediate the destruction of thymocytes and T cell-derived leukemia cells through a mechanism known as apoptosis. The characteristic feature of apoptosis is fragmentation of DNA at internucleosomal linkers through the activity of a specific endonuclease. In this study, an attempt was made to compare dexamethasone-induced apoptosis in two T cell-derived human leukemia lines (CEM-C1 and CEM-C7) to the cell killing brought about by selected cytotoxic agents. In the CEM-C7 cell line (dexamethasone-sensitive), apoptosis was induced not only by dexamethasone but by actinomycin D, cycloheximide, and 25-OH cholesterol. In the CEM-C1 cell line (dexamethasone-resistant) cycloheximide, 25-OH cholesterol, or cell starvation could induce apoptosis. It appears that in leukemic cells apoptosis may be induced by a variety of unrelated toxic agents and is not limited to glucocorticoids.

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Changes in nuclear protein pattern by glucocorticoid treatment of lymphoid cells

Cited by 5 publications

References 21 publications

A biochemical hallmark of apoptosis: Internucleosomal degradation of the genome

A biochemical hallmark of apoptosis: Internucleosomal degradation of the genome

Identification, purification, and characterization of a calcium-dependent endonuclease (NUC18) from apoptotic rat thymocytes. NUC18 is not histone H2B.

Apoptosis: mode of cell death induced in T cell leukemia lines by dexamethasone and other agents

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