Changes in Non-Volatile and Volatile Metabolites Associated with Heterosis in Tea Plants (<i>Camellia sinensis</i>)

Wang, Pengjie; Gu, Mengya; Shao, Shuxian; Chen, Xiaomin; Hou, Binghao; Ye, Naixing; Zhang, Xingtan

doi:10.1021/acs.jafc.1c08248

Cited by 33 publications

(26 citation statements)

References 45 publications

(88 reference statements)

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“…To explore the regulation of allelic parental bias and chromatin accessibility in tea plant heterosis, the genome‐wide RNA‐seq and ATAC‐seq analyses were performed and associated with the heterosis metabolites determined in our previous research (Wang, et al., 2022b; Figure 1a; Tables S1 and S2). Based on our recently published high‐quality parental genomes (Wang, et al., 2021b; Zhang et al., 2021) and AlleleFinder software (https://github.com/sc-zhang/AlleleFinder), we identified 11 592 alleles between maternal TGY and paternal HD and calculated their allelic expression in tea hybrids JGY and HGY (Table S3).…”

Section: Resultsmentioning

confidence: 99%

“…However, research on the tea plant heterosis is still poorly understood. We recently resolved high‐quality reference genomes of TGY (Zhang et al., 2021) and HD (Wang, et al., 2021b), the core parents of oolong tea breeding, and systematically identified the heterotic metabolites of JGY and HGY, the artificial hybrids of oolong tea with the largest planting area in China (Wang, et al., 2022b). Here, we explored the ASE and chromatin accessibility in hybrids to provide new insights into the formation and regulation of tea plant heterosis.…”

Section: Discussionmentioning

confidence: 99%

“…Terpene volatiles and purine alkaloids are the representative metabolites of tea, and play a vital role in the growth and quality formation of tea plants (Liao et al., 2022). Both terpenes and purine nucleotides in hybrids JGY and HGY have been found to exhibit significant heterosis (Wang, et al., 2022b). Multiple ASEGs on two metabolic pathways in hybrids were regulated by different parental biases.…”

Section: Discussionmentioning

confidence: 99%

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Allele‐specific expression and chromatin accessibility contribute to heterosis in tea plants (Camellia sinensis)

Wang

et al. 2022

The Plant Journal

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SUMMARY Heterosis is extensively used to improve crop productivity, yet its allelic and chromatin regulation remains unclear. Based on our resolved genomes of the maternal TGY and paternal HD, we analyzed the contribution of allele‐specific expression (ASE) and chromatin accessibility of JGY and HGY, the artificial hybrids of oolong tea with the largest cultivated area in China. The ASE genes (ASEGs) of tea hybrids with maternal‐biased were mainly related to the energy and terpenoid metabolism pathways, whereas the ASEGs with paternal‐biased tend to be enriched in glutathione metabolism, and these parental bias of hybrids may coordinate and lead to the acquisition of heterosis in more biological pathways. ATAC‐seq results showed that hybrids have significantly higher accessible chromatin regions (ACRs) compared with their parents, which may confer broader and stronger transcriptional activity of genes in hybrids. The number of ACRs with significantly increased accessibility in hybrids was much greater than decreased, and the associated alleles were also affected by differential ACRs across different parents, suggesting enhanced positive chromatin regulation and potential genetic effects in hybrids. Core ASEGs of terpene and purine alkaloid metabolism pathways with significant positive heterosis have greater chromatin accessibility in hybrids, and were potentially regulated by several members of the MYB, DOF and TRB families. The binding motif of CsMYB85 in the promoter ACR of the rate‐limiting enzyme CsDXS was verified by DAP‐seq. These results suggest that higher numbers and more accessible ACRs in hybrids contribute to the regulation of ASEGs, thereby affecting the formation of heterotic metabolites.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Allele‐specific expression and chromatin accessibility contribute to heterosis in tea plants (Camellia sinensis)

Wang

et al. 2022

The Plant Journal

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“…Hybrid F1 generation TY871 had a higher correlation with the parent TF ( Figure 2 A), and hybrid F1 generation TY398 had a higher correlation with the parent GH ( Figure 2 A), which was also confirmed by the rice quality data ( Table 2 ). Metabolites are the strongest contributors to plant phenotypes and reflect the regulation of upstream genes and downstream manifestations of heterosis [ 22 ]. Changes in most metabolites were similar between the TY398 and TY871 varieties ( Figure S1 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome and Metabolome Analysis Provides Insights into the Heterosis of Yield and Quality Traits in Two Hybrid Rice Varieties (Oryza sativa L.)

Zhou

Sun

et al. 2022

IJMS

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Heterosis is a common biological phenomenon that is useful for breeding superior lines. Using heterosis to increase the yield and quality of crops is one of the main achievements of modern agricultural science. In this study, we analysed the transcriptome and metabolome of two three-line hybrid rice varieties, Taiyou 871 (TY871), and Taiyou 398 (TY398) and the parental grain endosperm using RNA-seq (three biological repeats per variety) and untargeted metabolomic (six biological repeats per variety) methods. TY871 and TY398 showed specific heterosis in yield and quality. Transcriptome analysis of the hybrids revealed 638 to 4059 differentially expressed genes in the grain when compared to the parents. Metabolome analysis of the hybrids revealed 657 to 3714 differential grain metabolites when compared to the parents. The honeydew1 and grey60 module core genes Os04g0350700 and Os05g0154700 are involved in the regulation of awn development, grain size, and grain number, as well as the regulation of grain length and plant height, respectively. Rice grain length may be an important indicator for improving the quality of three-line hybrid rice. In addition, the rice quality-related metabolite NEG_M341T662 was highly connected to the module core genes Os06g0254300 and Os03g0168100. The functions of Os06g0254300 and Os03g0168100 are EF-hand calcium binding protein and late embroideries absolute protein repeat containing protein, respectively. These genes may play a role in the formation of rice quality. We constructed a gene and metabolite coexpression network, which provides a scientific basis for the utilization of heterosis in producing high-yield and high-quality hybrid rice.

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“…Heterosis is the phenomenon in which a hybrid progeny has greater biomass and higher yield and quality than the parents; volatile heterosis was obvious in F1 hybrids [ 9 ]. The catechins and free amino acids in hybrids showed negative heterosis compared to its maternal cultivar TGY [ 10 ]. Earlier, we crossed Chinese-type clone-cultivars ‘Sukoi’, ‘Tambi-1’, and ‘Tambi-2’ with Assam-type clone-cultivars ‘TRI-2025’, ‘Suka Ati-40’, and ‘Kiara-8’ and produced some hybrid progenies.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional Comparison of New Hybrid Progenies and Clone-Cultivars of Tea (Camellia sinensis L.) Associated to Catechins Content

Widhianata

Basunanda

Supriyadi

et al. 2022

Plants

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Heterosis or hybrid vigor is the improved performance of a desirable quality in hybrid progeny. Hybridization between high-productive Assam type and high-quality Chinese type clone-cultivar is expected to develop elite tea plant progenies with high quality and productivity. Comparative transcriptomics analyses of leaves from the F1 hybrids and their parental clone-cultivars were conducted to explore molecular mechanisms related to catechin content using a high-throughput next-generation RNA-seq strategy and high-performance liquid chromatography (HPLC). The content of EGCG (epigallocatechin gallate) and C (catechin) was higher in ‘Kiara-8’ × ‘Sukoi’, ‘Tambi-2’ × ‘Suka Ati’, and ‘Tambi-2’ × ‘TRI-2025’ than the other hybrid and clone-cultivars. KEGG (Kyoto Encyclopedia of Genes and Genomes) and GO (Gene Ontology) analysis found that most pathways associated with catechins content were enriched. Significant differentially expressed genes (DEGs) mainly associated with phenylpropanoid, flavonoid, drug metabolism-cytochrome P450, and transcription factor (MYB, bHLH, LOB, and C2H2) pathways appeared to be responsible for the high accumulation of secondary metabolites in ‘Kiara-8’ × ‘Sukoi’, ‘Tambi-2’ × ‘Suka Ati’, and ‘Tambi-2’ × ‘TRI-2025’ as were detected in EGCG and catechin content. Several structural genes related to the above pathways have been obtained, which will be used as candidate genes in the screening of breeding materials.

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Changes in Non-Volatile and Volatile Metabolites Associated with Heterosis in Tea Plants (Camellia sinensis)

Cited by 33 publications

References 45 publications

Allele‐specific expression and chromatin accessibility contribute to heterosis in tea plants (Camellia sinensis)

Allele‐specific expression and chromatin accessibility contribute to heterosis in tea plants (Camellia sinensis)

Transcriptome and Metabolome Analysis Provides Insights into the Heterosis of Yield and Quality Traits in Two Hybrid Rice Varieties (Oryza sativa L.)

Transcriptional Comparison of New Hybrid Progenies and Clone-Cultivars of Tea (Camellia sinensis L.) Associated to Catechins Content

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