Changes in non-histone chromosomal proteins during the development of potato tubers; their involvement in wound- and hormone-induced processes

Kahl, Günter; Schäfer, Willi; Wechselberger, Manfred

doi:10.1093/oxfordjournals.pcp.a075921

Cited by 13 publications

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“…Wounded tissues contain more NHCP than resting ones [13,22]. However, there is evidence that wounding per se or the hormones released from inactive form by wounding induces quantitative and qualitative changes in the nonhistone chromosomal proteins (NHCP).…”

Section: Introductionmentioning

confidence: 99%

Auxin-induced changes in chromosomal protein phosphorylation in wounded potato tuber parenchyma

Schäfer

Kahl

1981

Plant Mol Biol

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White potato tuber tissue reacts upon wounding with a rapid increase in activity of both chromatin-bound DNA-dependent RNA polymerase I and II as well as protein phosphokinase. This enhancement is more pronounced if 0.1 mM of the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) is added shortly after wounding. The effect of the hormone on protein kinases becomes evident only after a lag phase of about 10h and lasts throughout the wound-healing period. Different protein kinases with different substrate specificity (i.e. histone, phosvitin, casein phosphokinases) are distinctly more active in auxin-treated tissues. The phosphate is apparently introduced into proteins via seryl and threonyl bonds. Acyl or histidyl phosphates are not involved.The properties of protein phosphokinases are virtually identical in wounded and auxin-treated tissues. However, the pattern of chromosomal proteins and the pattern of their phosphorylation in hormone-treated tissues is different from those in wounded ones. A drastic stimulation of phosphorylation of both high and low-molecular weight chromosomal proteins is characteristic for auxin-treated cells.

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Section: Introductionmentioning

confidence: 99%

Auxin-induced changes in chromosomal protein phosphorylation in wounded potato tuber parenchyma

Schäfer

Kahl

1981

Plant Mol Biol

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“…al., 1976), and the cell cycle stage specific transcription of the histone genes Jansing et al, 1977). These observations, including the fact that a substantial part of NHCP turnover in differentiating cells (Stambolova & Angelova, 1979;Kahl et al, 1979;Guguen-Gillouzo et al, 1979) and that certain NHCP are tissue specific (Teng et al, 1971;Miyazaki et al, 1978), strongly suggest that NHCP are involved in the regulation of gene expression.…”

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confidence: 99%

Carboxyl methylation of nonhistone chromosomal proteins

Quick¹,

Orchard²,

Duerre³

1981

Biochemistry

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The in vitro methylation of nonhistone chromosomal proteins (NHCP) was investigated in nuclei isolated from the brain, liver, and thymus of 6-8-day-old rats. After the nuclei were incubated in the presence of 20 micron M S-adenosyl-L-[methyl-3H] methionine (1 Ci/mmol), the NHCP were separated from histones on hydroxylapatite and fractionated further on sodium dodecyl sulfate-acrylamide slab gel electrophoresis. After the gels were dried, autoradiography was used to detect [3H] methyl groups associated with these proteins. Four NHCP from the liver and thymus were methylated, while six methylated proteins were detected from the brain. None of the methylated proteins in these tissues corresponded with those from other organs, except for the component with a molecular weight of 66 000. It was evident that the methyl groups were esterified to the free carboxyl groups of NHCP since they are heat labile, yielding [3H]-methanol. The carboxyl-methylated NHCP from these organs were tightly bound to chromatin. Nucleoplasm and loosely associated NHCP were essentially devoid of methylated proteins. The carboxyl methylation of NHCP was verified in vivo. Six-day-old rats were given L-[methyl-3H]methionine (7 mCi/mmol) by intraperitoneal injection. The rats were killed at varying time periods and the NHCP isolated from gradient purified nuclei. Chromosomal nonhistone proteins, particularly from the liver, contained significant amounts of alkaline labile [(3)H] methyl groups.

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Gene Action Changes during Nonvertebrate Differentiation

Dillon¹

1983

The Inconstant Gene

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Changes in non-histone chromosomal proteins during the development of potato tubers; their involvement in wound- and hormone-induced processes

Cited by 13 publications

References 0 publications

Auxin-induced changes in chromosomal protein phosphorylation in wounded potato tuber parenchyma

Auxin-induced changes in chromosomal protein phosphorylation in wounded potato tuber parenchyma

Carboxyl methylation of nonhistone chromosomal proteins

Gene Action Changes during Nonvertebrate Differentiation

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