Changes in nitrogen metabolism and antioxidant enzyme activities of maize tassel in black soils region of northeast China

Xu, Hua; Lü, Yan; Xie, Zhongkui; Song, Fuqiang

doi:10.3389/fpls.2014.00515

Cited by 15 publications

(10 citation statements)

References 26 publications

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“…On the contrary, low N attenuates ROS scavenging system and increases oxidative stress in leaves ( Ramalho et al, 1998 ). Our results are consistent with those findings that low N led to significant higher lipid peroxidation and H 2 O 2 content compared to moderate and high N. Besides, water scarcity aggravated oxidative injure to membrane at low N. Xu et al (2014) proposed that proline could protect antioxidative enzymes. Similarly, in this study, the responses of CAT and APX activities to water stress were consistent with proline content (except moderate N).…”

Section: Discussionsupporting

confidence: 93%

Nitrogen Metabolism in Adaptation of Photosynthesis to Water Stress in Rice Grown under Different Nitrogen Levels

et al. 2017

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To investigate the role of nitrogen (N) metabolism in the adaptation of photosynthesis to water stress in rice, a hydroponic experiment supplying with low N (0.72 mM), moderate N (2.86 mM), and high N (7.15 mM) followed by 150 g⋅L-1 PEG-6000 induced water stress was conducted in a rainout shelter. Water stress induced stomatal limitation to photosynthesis at low N, but no significant effect was observed at moderate and high N. Non-photochemical quenching was higher at moderate and high N. In contrast, relative excessive energy at PSII level (EXC) was declined with increasing N level. Malondialdehyde and hydrogen peroxide (H2O2) contents were in parallel with EXC. Water stress decreased catalase and ascorbate peroxidase activities at low N, resulting in increased H2O2 content and severer membrane lipid peroxidation; whereas the activities of antioxidative enzymes were increased at high N. In accordance with photosynthetic rate and antioxidative enzymes, water stress decreased the activities of key enzymes involving in N metabolism such as glutamate synthase and glutamate dehydrogenase, and photorespiratory key enzyme glycolate oxidase at low N. Concurrently, water stress increased nitrate content significantly at low N, but decreased nitrate content at moderate and high N. Contrary to nitrate, water stress increased proline content at moderate and high N. Our results suggest that N metabolism appears to be associated with the tolerance of photosynthesis to water stress in rice via affecting CO2 diffusion, antioxidant capacity, and osmotic adjustment.

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Section: Discussionsupporting

confidence: 93%

Nitrogen Metabolism in Adaptation of Photosynthesis to Water Stress in Rice Grown under Different Nitrogen Levels

et al. 2017

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“…After scanning the reference genome of peanut, the Rsa I and Eco RV-HF® (NEB, Ipswich, MA) enzymes were selected to digest the genomic DNA. The protocol of the SLAF-library construction has been previously described [19, 58]. The DNA fragments with indices and adaptors (SLAFs) of 314–414 bp were excised and diluted for pair-end sequencing using the Illumina HighSeq 2500 platform according to the Illumina sample preparation guide (Illumina, Inc., San Diego, CA) at the Biomarker Technologies Corporation (Beijing, China).…”

Section: Methodsmentioning

confidence: 99%

“…Allelic SNP markers have the advantages of high frequency of occurrence in peanut genome [15, 16]. Combined with the next generation sequencing (NGS), a number of SNP-based genotyping technologies, especially SLAF-seq, have been applied to HDGM construction and QTL analysis in several species, such as sesame [17], soybean [18], cucumber [19, 20], cotton [21] and peanut [8, 22, 23]. In this study, a HDGM for cultivated peanut has been constructed based on SNP and SSR markers.…”

Section: Introductionmentioning

confidence: 99%

QTL identification for seed weight and size based on a high-density SLAF-seq genetic map in peanut (Arachis hypogaea L.)

Zhang¹,

Hu²,

Miao³

et al. 2019

BMC Plant Biol

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BackgroundThe cultivated peanut is an important oil and cash crop grown worldwide. To meet the growing demand for peanut production each year, genetic studies and enhanced selection efficiency are essential, including linkage mapping, genome-wide association study, bulked-segregant analysis and marker-assisted selection. Specific locus amplified fragment sequencing (SLAF-seq) is a powerful tool for high density genetic map (HDGM) construction and quantitative trait loci (QTLs) mapping. In this study, a HDGM was constructed using SLAF-seq leading to identification of QTL for seed weight and size in peanut.ResultsA recombinant inbred line (RIL) population was advanced from a cross between a cultivar ‘Huayu36’ and a germplasm line ‘6–13’ with contrasting seed weight, size and shape. Based on the cultivated peanut genome, a HDGM was constructed with 3866 loci consisting of SLAF-seq and simple sequence repeat (SSR) markers distributed on 20 linkage groups (LGs) covering a total map distance of 1266.87 cM. Phenotypic data of four seed related traits were obtained in four environments, which mostly displayed normal distribution with varied levels of correlation. A total of 27 QTLs for 100 seed weight (100SW), seed length (SL), seed width (SW) and length to width ratio (L/W) were identified on 8 chromosomes, with LOD values of 3.16–31.55 and explaining phenotypic variance (PVE) from 0.74 to 83.23%. Two stable QTL regions were identified on chromosomes 2 and 16, and gene content within these regions provided valuable information for further functional analysis of yield component traits.ConclusionsThis study represents a new HDGM based on the cultivated peanut genome using SLAF-seq and SSRs. QTL mapping of four seed related traits revealed two stable QTL regions on chromosomes 2 and 16, which not only facilitate fine mapping and cloning these genes, but also provide opportunity for molecular breeding of new peanut cultivars with improved seed weight and size.

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“…often have been observed during abiotic stress (Sengupta et al, 2016;Vinocur and Altman, 2005;Xu et al, 2014;Yoshiba et al, 1997). GS plays an important role in proline production in the phloem and higher GS activity is observed under several abiotic stresses (Brugi ere et al, 1999).…”

Section: Molecular and Physiological Role Of Gs In Plantsmentioning

confidence: 99%

“…Plant GS is likely to synthesize the aa Glu and Gln through the GS‐GOGAT cycle. The aa Glu is essential for the biosynthesis of several osmoprotectants, in particular proline and polyamines, which assist in shielding cellular membranes and proteins, in addition to glutathione tripeptide (James et al., 2018), as the accumulation of a higher concentration of proline and polyamines often have been observed during abiotic stress (Sengupta et al., 2016; Vinocur and Altman, 2005; Xu et al., 2014; Yoshiba et al., 1997). GS plays an important role in proline production in the phloem and higher GS activity is observed under several abiotic stresses (Brugière et al., 1999).…”

Section: Introductionmentioning

confidence: 99%

Structural property, molecular regulation, and functional diversity of glutamine synthetase in higher plants: a data‐mining bioinformatics approach

2021

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Glutamine synthetase (GS; E.C.6.3.1.2) is a key enzyme in higher plants with two isozymes, cytosolic GS1 and plastidic GS2, and involves in the assimilation and recycling of NH 4 + ions and maintenance of complex traits such as crop nitrogen-use efficiency and yield. Our present understanding of crop nitrogen-use efficiency and its correlation with the functional role of the GS family genes is inadequate, which delays harnessing the benefit of this key enzyme in crop improvement. In this report, we performed a comprehensive investigation on the phylogenetic relationship, structural properties, complex multilevel gene regulation, and expression patterns of the GS genes to enrich present understanding about the enzyme. Our Gene Ontology and protein-protein interactions analysis revealed the functional aspects of GS isozymes in stress mitigation, aging, nucleotide biosynthesis/transport, DNA repair and response to metals. The insight gained here contributes to the future research strategies in developing climate-smart crops for global sustainability.

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Changes in nitrogen metabolism and antioxidant enzyme activities of maize tassel in black soils region of northeast China

Cited by 15 publications

References 26 publications

Nitrogen Metabolism in Adaptation of Photosynthesis to Water Stress in Rice Grown under Different Nitrogen Levels

Nitrogen Metabolism in Adaptation of Photosynthesis to Water Stress in Rice Grown under Different Nitrogen Levels

QTL identification for seed weight and size based on a high-density SLAF-seq genetic map in peanut (Arachis hypogaea L.)

Structural property, molecular regulation, and functional diversity of glutamine synthetase in higher plants: a data‐mining bioinformatics approach

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