Changes in mRNA and protein levels of human HtrA1, HtrA2 and HtrA3 in ovarian cancer

Narkiewicz, Joanna; Klasa-Mazurkiewicz, Dagmara; Żurawa-Janicka, Dorota; Skórko-Glonek, Joanna; Emerich, Janusz; Lipińska, Barbara

doi:10.1016/j.clinbiochem.2008.01.004

Cited by 66 publications

(81 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The expression of HtrA1 and HtrA3 is decreased in several cancers, while overexpression of HtrA1 in human cancers inhibits cell growth and proliferation (91)(92)(93)(94). These results suggest a tumor suppressor function for HtrA proteinases.…”

Section: High-temperature Requirement Proteinasesmentioning

confidence: 88%

Emerging roles of serine proteinases in tissue turnover in arthritis

Milner

Patel

Rowan

2008

Arthritis & Rheumatism

View full text Add to dashboard Cite

Section: High-temperature Requirement Proteinasesmentioning

confidence: 88%

Emerging roles of serine proteinases in tissue turnover in arthritis

Milner

Patel

Rowan

2008

Arthritis & Rheumatism

View full text Add to dashboard Cite

“…Total RNAs were extracted from endometrial tissues (20-50 mg) by a modified Chomczynski procedure using a 'Total RNA' purification kit (A&A Biotechnology, Poland) as described previously (25). The total RNA was quantified by a spectrophotometrical measurement at 260 nm.…”

Section: Methodsmentioning

confidence: 99%

“…The total RNA was quantified by a spectrophotometrical measurement at 260 nm. All RNA samples were DNase I-treated as described in (25).…”

Section: Methodsmentioning

confidence: 99%

“…DNase I-treated RNA samples were reverse-transcribed to cDNA as described previously (25). PCR amplification conditions were determined to obtain a similar amplification efficiency for both fragments and to receive an exponential phase of the amplification.…”

Section: Semi-quantitative Rt-pcrmentioning

confidence: 99%

“…Protein extracts were prepared from frozen tissue samples and the total protein concentration was determined by the Bradford method as described previously (25). Tissue lysates containing equal amounts of total protein were separated by SDS-PAGE.…”

Section: Semi-quantitative Rt-pcrmentioning

confidence: 99%

See 2 more Smart Citations

Expression of human HtrA1, HtrA2, HtrA3 and TGF-β1 genes in primary endometrial cancer

et al. 2009

View full text Add to dashboard Cite

Abstract. The HtrA family of serine proteases takes part in cellular stress response including heat shock, inflammation and cancer. Downregulation of human HtrA1 and HtrA3 genes has been reported in some cancers, including endometrial cancer (EC), suggesting a tumor-suppressor role for both genes. The mechanism of the HtrA function is not known, however, evidence exists showing that both HtrA1 and HtrA3 regulate biological processes by modulating TGF-ß signaling. In the presented study the expression of human HtrA1, HtrA2, HtrA3 and TGF-ß1 genes was examined in 124 endometrial tissue specimens including 88 cancers and 36 normal endometria. The expression of the tested genes was evaluated at mRNA and protein levels by semi-quantitative RT-PCR and Western blotting methods, respectively. Our results showed significant decrease of HtrA1 and HtrA3 mRNA and protein levels in EC compared to normal tissues. The most dramatic decrease was found for HtrA3 at both mRNA and protein levels (3.2-and 5.6-fold, respectively). Moreover, the HtrA3 protein (short isoform) was not detected in 19% of the cancers, and its level decreased from the premenopausal to the postmenopausal group. The HtrA2 protein levels were significantly lower in EC tissues compared to normal tissues. We also found a significant increase of the TGF-ß1 protein level in EC as well as a significant negative correlation between HtrA1/2/3 and TGF-ß1 relative protein levels. Our results showing downregulation of HtrA1 and HtrA3 gene expression support previous studies suggesting a tumor suppressor role for these genes. Furthermore, our data suggest that HtrA2 may be involved in EC development as well as suggest the involvement of HtrA1, HtrA2 and HtrA3 in the inhibition of TGF-ß signaling in endometrial tissues.

show abstract