Changes in motility, ultrastructure, and fertilization capacity of striped bass Morone saxatilis spermatozoa following cryopreservation

Ultrastructure of fresh and post thawed sperm of pejerrey Odontesthes bonariensis (Atheriniformes)

“…After cryopreservation treatment, swollen of the head, flagellum which often containing cytoplasmatic vesicles, and the tail surrounding the head were found. In other fish orders, another kind of alterations were reported as lost or dilated mitochondria, swollen midpiece, and broken or double tails (Taddei et al, 2001;He & Woods, 2004;Alavi et al, 2008). All these cellular damages could be compromising the sperm motility, but does not necessary mean that the fertilization rate is affected.…”

“…For example, ruptured plasmatic membranes in the spermatozoa head, midpiece, and flagellum, as well as swollen mitochondria and broken axoneme were frequently found after thawing (Gwo et al, 1992;Lahnsteiner et al, 1996;Yao et al, 2000;Taddei et al, 2001). Besides, the examination of sperm morphology at ultrastructural level provides useful information to develop cryopreservation protocols (He & Woods, 2004), and also for doing phylogenetic analysis; Mattei, 1991;Gwo et al, 2004a, b;Franҫa et al, 2007;Jamieson, 2009). Between the methods that are used to investigate cellular damages, electron microscopy techniques provide detailed information on the subcellular ultrastructure representing a useful tool for the evaluation of morphological changes that can affect sperm functionality (Taddei et al, 2001).…”

DNA fragmentation and membrane damage of bocachico Prochilodus magdalenae (Ostariophysi: Prochilodontidae) sperm following cryopreservation with dimethylsulfoxide and glucose

“…In other words, it do not decreased the freezing points (Kopeika & Kopeika, 2008), that permits the formation of ice with greater efficiency causes membrane damages during freezing or thawing (Chao & Liao, 2001). In a similar case, during the cryopreservation of striped bass Morone saxatilis, it was detected by a scanning electron microscope that with a minimum concentration of DMSO (2.5%), large damages in the plasmatic membrane occurred, suggesting that the damages were due to the low or nonexistent protection that the sperm received from the cryoprotectant, associating it with the formation of intracellular ice crystals (He & Woods, 2004).…”

“…15%) is capable of producing greater membrane damage than DMSO 10% and 5%. In striped bass, when three levels of DMSO (2.5%, 5%, 10%) were tested, it was found that the greatest damage to the plasmatic membrane was generated under the maximum concentration, being statistically different (P<0.05) from the mean concentration (DMSO 5%, resulting in the least membrane damage) (He & Woods, 2004). In this respect, Kopeika & Kopeika (2008) signal that the movement rate of water through the cellular membrane is proportional to the difference in the concentration of the solution (e.g.…”

Adição de alanina, glicina e glutamina ao meio crioprotetor seminal de garanhões Mangalarga Marchador

“…A glicina tem sido usada com sucesso como crioprotetor não-penetrante para criopreservar espermatozoides e aumentar a motilidade pós-descongelamento (Kundu et al, 2001;He & Woods 2003). He & Woods (2004b) observaram que a glicina protege a mitocôndria e seu conteúdo de ATP, mas não protege a integridade da membrana plasmática espermática no pós-descongelamento. O mecanismo de ação da glicina é desconhecido.…”