Changes in miRNA levels of sperm and small extracellular vesicles of seminal plasma are associated with transient scrotal heat stress in bulls

Alves, Maíra Bianchi Rodrigues; Arruda, Rubens Paes de; Batissaco, Leonardo; Garcia‐Oliveros, Laura Nataly; Gonzaga, Vitor Hugo Guilger; Nogueira, Vinícius José Moreira; Almeida, Felipe P.; Pinto, Sâmara Cristine Costa; Andrade, Gabriella Mamede; Perecin, Felipe; Silveira, Juliano Coelho da; Celeghini, Eneiva Carla Carvalho

doi:10.1016/j.theriogenology.2020.11.015

Cited by 30 publications

(22 citation statements)

References 65 publications

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“…The remaining differentially expressed miRNAs identified in our studies, such as the miR-22a-5p, miR-126, miR-22a, and miR-221 have also been earlier reported in semen samples of zebrafish and other species ( Smorag et al, 2012 ; Jia et al, 2015 ; Tao et al, 2018 ; Alves et al, 2021 ). However, the functional role of these miRNAs and target mRNAs has not yet been fully elucidated.…”

Section: Discussionsupporting

confidence: 80%

GH Overexpression Alters Spermatic Cells MicroRNAome Profile in Transgenic Zebrafish

et al. 2021

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Overexpression of growth hormone (GH) in gh-transgenic zebrafish of a highly studied lineage F0104 has earlier been reported to cause increased muscle growth. In addition to this, GH affects a broad range of cellular processes in transgenic fish, such as morphology, physiology, and behavior. Reports show changes such as decreased sperm quality and reduced reproductive performance in transgenic males. It is hypothesized that microRNAs are directly involved in the regulation of fertility potential during spermatogenesis. The primary aim of our study was to verify whether gh overexpression disturbs the sperm miRNA profile and influences the sperm quality in transgenic zebrafish. We report a significant increase in body weight of gh-transgenic males along with associated reduced sperm motility and other kinetic parameters in comparison to the non-transgenic group. MicroRNA transcriptome sequencing of gh-transgenic zebrafish sperms revealed expressions of 186 miRNAs, among which six miRNA were up-regulated (miR-146b, miR-200a-5p, miR-146a, miR-726, miR-184, and miR-738) and sixteen were down-regulated (miR-19d-3p, miR-126a-5p, miR-126b-5p, miR-22a-5p, miR-16c-5p, miR-20a-5p, miR-126b-3p, miR-107a-3p, miR-93, miR-2189, miR-202–5p, miR-221–3p, miR-125a, miR-125b-5p, miR-126a-3p, and miR-30c-5p) in comparison to non-transgenic zebrafish. Some of the dysregulated miRNAs were previously reported to be related to abnormalities in sperm quality and reduced reproduction ability in other species. In this study, an average of 134 differentially expressed miRNAs-targeted genes were predicted using the in silico approach. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis demonstrated that the genes of affected pathways were primarily related to spermatogenesis, sperm motility, and cell apoptosis. Our results suggested that excess GH caused a detrimental effect on sperm microRNAome, consequently reducing the sperm quality and reproductive potential of zebrafish males.

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Section: Discussionsupporting

confidence: 80%

GH Overexpression Alters Spermatic Cells MicroRNAome Profile in Transgenic Zebrafish

et al. 2021

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“…During winter season, although mid-piece sperm abnormalities were significantly (p<0.05) higher in PW (10.0±0.99) than in PS (7.35±0.78) bulls, total sperm abnormalities in bulls of both the groups were within the normal prescribed limits for further processing of semen for freezing. Alves et al (2021) reported that scrotal heat stress affected male fertility by increasing sperm morphological abnormalities. In summer, reactive oxygen species production significantly increases in sperm, leading to lipid peroxidation and major sperm defects (Nichi et al 2006).…”

Section: Resultsmentioning

confidence: 99%

Shelter designing and season affects scrotal thermal profile vis-a-vis semen quality in Frieswal breeding bulls

Sirohi

Pande

Chand

et al. 2022

Indian J of Anim Sci

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Twenty-four Frieswal bulls (Holstein Friesian × Sahiwal) were randomly distributed according to their housing: animals housed individually in sheds with partition (n = 12; PS) and without partition (n = 12; PW) wall. The present study revealed that the alteration in design of bull housing affected THI leading to changes in scrotal thermal gradient in crossbred Frieswal bulls during different seasons. Scrotal thermal gradient was higher in PW bulls than PS bulls during afternoon period in both the seasons. The semen quality was better in the bulls with higher thermal gradient in well-ventilated sheds during hot-humid season. The season and design of bull housing affected sperm morphology during different seasons. Higher total antioxidant capacity was estimated in the bulls of PW than in bulls of PS during hot-humid season, but no difference was noticed during winter. It was concluded that the season and design of housing can affect scrotal thermal gradient and semen quality in crossbred breeding bulls.

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“…miRNA profiles in seminal plasma are correlated with specific spermatogenic defects and, more broadly, in male infertility [ 217 , 218 , 219 ]. Effects of environmental exposures on spermatogenesis, such as heat stress, may also be reflected by the miRNA profile of seminal plasma [ 220 ]. tsRNAs were found to be abundant in human semen-derived exosomes [ 221 ].…”

Section: Sncrnas In Sperm Seminal Plasma Follicular Fluid and Embryo Culture Medium As Biomarkers For Fertility And Embryo Development Pomentioning

confidence: 99%

Small Noncoding RNAs in Reproduction and Infertility

et al. 2021

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Infertility has been reported as one of the most common reproductive impairments, affecting nearly one in six couples worldwide. A large proportion of infertility cases are diagnosed as idiopathic, signifying a deficit in information surrounding the pathology of infertility and necessity of medical intervention such as assisted reproductive therapy. Small noncoding RNAs (sncRNAs) are well-established regulators of mammalian reproduction. Advanced technologies have revealed the dynamic expression and diverse functions of sncRNAs during mammalian germ cell development. Mounting evidence indicates sncRNAs in sperm, especially microRNAs (miRNAs) and transfer RNA (tRNA)-derived small RNAs (tsRNAs), are sensitive to environmental changes and mediate the inheritance of paternally acquired metabolic and mental traits. Here, we review the critical roles of sncRNAs in mammalian germ cell development. Furthermore, we highlight the functions of sperm-borne sncRNAs in epigenetic inheritance. We also discuss evidence supporting sncRNAs as promising biomarkers for fertility and embryo quality in addition to the present limitations of using sncRNAs for infertility diagnosis and treatment.

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Changes in miRNA levels of sperm and small extracellular vesicles of seminal plasma are associated with transient scrotal heat stress in bulls

Cited by 30 publications

References 65 publications

GH Overexpression Alters Spermatic Cells MicroRNAome Profile in Transgenic Zebrafish

GH Overexpression Alters Spermatic Cells MicroRNAome Profile in Transgenic Zebrafish

Shelter designing and season affects scrotal thermal profile vis-a-vis semen quality in Frieswal breeding bulls

Small Noncoding RNAs in Reproduction and Infertility

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