Changes in Membrane Fluidity and Fatty Acid Composition ofPseudomonas putidaCN-T19 in Response to Toluene

Pseudomonas putidaNCTC 10936 Balances Membrane Fluidity in Response to Physical and Chemical Stress by Changing the Saturation Degree and theTrans/cisRatio of Fatty Acids

“…16,23,24) However, in growing cells of Pseudomonas strains 16,23) both changes of the saturation degree of the fatty acids by de novo synthesis of saturated and unsaturated fatty acids via the anaerobic pathway 25,26) and conversion of cis into trans fatty acids by a cis-trans isomerase 27) can occur in parallel. Therefore the elucidation of their contribution to homeoviscousis is difficult.…”

“…The resulting disturbance of their order can be measured as an increase of the lipid fluidity. 23) ) Resting cells respond as in the incubations with increasing temperatures, with major changes in the trans/cis ratio, but not in the degree of saturation, because again de novo synthesis of fatty acids is impossible (4-CP cannot be used as a carbon and energy source in acetate-grown cells). Within 2 h, 10 mM 4-CP induced the highest conversion of cis to trans fatty acids, but this was accompanied by a lower decrease in fluidity than that induced by raising the incubation temperature (cf.…”

A comparative analysis of microflora during biofilm development on grape seeds exposed to methanol in a biofilter

“…This genus was found in biofilters used to evaluate the methanol and toluene degradation (Zilli et al 2000). Pseudomonas putida (Zahir et al 2006;Heipieper et al 2007), Pseudomonas fluorescens (Isken and de Bont 1998), Sphingomonas paucimobilis, and Acetinobacter baumanii are capable of tolerating organic solvents, which they consume as their sole carbon and energy source (Hartig et al 2005;Junker et al 2001;Kim et al 2002;Wacket 2003). In contrast, gram-positive bacteria were not found in GS exposed to methanol, which is likely a result of the predominance of gram-negative bacteria (Juteau et al 1999).…”

Characterization of combinatorial patterns generated by multiple two‐component sensors in E. coli that respond to many stimuli

“…Temperature, pH, various chemicals, oxygen, and osmolarity all affect membrane properties, such as fluidity, permeability, potential, and lipid and composition, and protein composition (Dombek and Ingram, 1984b; Farr et al, 1988; Garbe et al, 2000; Kim et al, 2002; Sinensky, 1974; Zilberstein et al, 1984). Altered by a particular stimulus or micro-environment, the membrane may act as a sensor, whose combined set of properties is sensed by a set of two-component systems, and whose patterns are then used as inputs to various cellular programs.…”