Changes in malondialdehyde concentrations and glutathione peroxidase activity in purebred Arabian mares with endometritis

Yaralioglu-Gurgoze, Sema; Çetin, Hayrettin; Çen, Osman; Yılmaz, Seval; Atlı, Mehmet Osman

doi:10.1016/j.tvjl.2004.04.002

Cited by 19 publications

(13 citation statements)

References 17 publications

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“…This is indicated by an increase in CL activity, which accompanies a clear decrease in SOD and GPx activity, as well as decreased TAS in the blood. These results are in the agreement with prior publication by Yaralioglu-Gurgoze et al (30), who have shown a significant decrease in erythrocytic GPx in mares with endometritis in comparison to healthy animals. This phenomenon was associated with a clear increase in malonyl dialdehyde (MDA) content, which is one of the final products of the peroxidation of lipid cellular membranes, which additionally confirms an inadequate elimination of ROS by antioxidant compounds.…”

Section: Discussionsupporting

confidence: 94%

Oxidant/Antioxidant Status Assessment of Blood in Selected Equine Diseases

Krumrych

Zbylut

Gołyński

et al. 2013

Bulletin of the Veterinary Institute in Pulawy

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The objective of the study was to assess ratio of oxidation to antioxidation in the blood of horses in selected equine diseases. The study was conducted on 47 Polish half-breed horses (36 mares, six stallions, and five geldings) aged 2 to 16 years. Nineteen mares were diagnosed with endometritis, seven horses with symptoms of colic, and six with upper respiratory tract infection. The remaining clinically healthy horses (n=15) served as control. The following parameters were measured: oxygen metabolism in neutrophils using a chemiluminescence (CL) method, total antioxidant status (TAS) of blood plasma, as well as superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase activity in whole blood. The results demonstrate increased CL of neutrophils in mares with endometritis, as well as in horses with symptoms of colic. This finding was associated with decreased values of SOD, GPx, and TAS (chiefly in mares with endometritis), which suggests oxidative stress. These results underscore the importance of a broader use of oxidant/antioxidant status assessment in studies of the pathogenesis and pathomechanisms of equine diseases.

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Section: Discussionsupporting

confidence: 94%

Oxidant/Antioxidant Status Assessment of Blood in Selected Equine Diseases

Krumrych

Zbylut

Gołyński

et al. 2013

Bulletin of the Veterinary Institute in Pulawy

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“…However there are a limited number of studies on the action of MDA during inflammatory processes. Yaralioglu-Gurgoze et al (26) have reported that in mares with endomteritis MDA levels were significantly higher compared to that of healthy mares. Yoruk et al (27), experimentally induced staphylococcal maxillary sinusitis in rabbits and observed that treatment with antibiotics revealed lower tissue concentrations of MDA compared to methylprednisolone treated or untreated animals.…”

Section: Discussionmentioning

confidence: 94%

The effect of preoperative antibiotic or antibiotic + vitamin C administrations on the inflammatory and oxidative state in the rabbits with experimentally induced pyometra

BECERİKLİSOY¹

2013

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Summary:In this study, rabbits were used as a model for canine pyometra to test the effects of preoperative therapy in the reduction of postoperative stress. Pyometra were induced in 18 healthy rabbits by the operative administration of Pasteurella multocida inoculum. Beginning on the third day after inoculation Group I (n=6) received enrofloxacin (2,5 mg/kg, s.c.), Group II (n=6) recieved enrofloxacin (2,5 mg/kg, s.c.) + vitamin C (100 mg, s.c.) injections for three days. Ovariohysterectomy were performed in groups I and II following this medical treatment. Group III (n=6) as the control group, did not recieve any medical therapy and were operated on the third day after inoculation. Blood samples were collected before inoculation, on the day of operation, 24 th h, 72 th h and at 7 th day following the operations. Ceruloplasmin (CERU), malondialdehyde (MDA), vitamin C, haptoglobin (Hp) and serum amyloid-A (SAA) levels were analyzed. In the postoperative 72 th h period mean CERU level of Group III were found significantly higher (p<0,05) than Group I and II. Mean MDA level were significantly higher in Group III on the day of operation compared to the treatment groups (p<0,05). SAA levels rised in all of the groups following inoculation, on the day of operation (p<0,05) which is followed by a gradual decline until the 7 th day. Overall CERU and MDA levels tend to be higher in the control group compared to the treatment groups whereas Hp and SAA levels were not significantly different. These findings may indicate that preoperative therapy may reduce the oxidative stress mildly however it did not significantly alter the acute phase protein response definitively and a significant positive effect of both preoperative treatments compared to the control group could not be pronounced.Keywords: Acute phase proteins, antioxidants, ovariohysterectomy, pyometra, rabbit. Tavşanlarda deneysel oluşturulan pyometra olgularında, preoperatif antibiyotik veya antibiyotik + C vitamini enjeksiyonlarının yangısal ve oksidatif duruma olan etkileriÖzet: Bu çalışmada pyometra operasyonu sonrası stresin azaltılmasında preoperatif sağaltımın etkisini araştırmak amacıyla tavşanlar model olarak kullanıldı. Bu amaçla onsekiz sağlıklı tavşanda Pasteurella multocida' nın operatif yöntemle uterusa inokulasyonu gerçekleştirildi. İnokulasyondan sonra 3. günden başlanarak Grup I' e (n=6) enrofloksasin (2,5 mg/kg, s.c.), Grup II' ye (n=6) aynı dozda enrofloksasin ve vitamin C (100 mg, s.c.) enjeksiyonları 3 gün süreyle devam ettirilmiş ve bundan sonra ovaryohisterektomi uygulanmıştır. Kontrol grubu olan Grup III' e (n=6) medikal sağaltım uygulanmamış ve inokulasyondan sonra üçüncü günde ovaryohisterektomi uygulanmıştır. Kan örnekleri inokulasyondan önce, ovaryohisterektomi gününde, postoperatif 24. saat, 72. saat ve 7. günde alınmıştır. Bu örneklerde serüloplazmin (CERU), malondialdehit (MDA), vitamin C, haptoglobin (Hp) ve Serum Amiloid A (SAA) düzeyleri belirlenmiştir. Serüloplazmin bütün gruplarda inokulasyonu takiben yükselirken Grup I ve II' de...

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“…In agreement with these researches, in the present study, it was ascertained that the MDA levels of the cows with endometritis were significantly higher than those of the healthy animals, and the increase in MDA levels were higher as the severity of endometritis increased. This increase is possibly, due to the excessive production of the hydroxyl (OH) radicals, which cause damage to the DNA and cell membrane, during infection in mares (Yaralioglu-Gurgoze et al, 2005).…”

Section: Levels Of Specific Oxidative Stress Parameters In Groupsmentioning

confidence: 99%

“…While cases of clinical endometritis are scored according to the character and amount of uterine discharge (Sheldon et al, 2006;Williams et al, 2005), cases of subclinical endometritis are diagnosed by cytological examination (Barlund et al, 2008). In endometritis cases, the immune system is activated (Yaralioglu-Gurgoze et al, 2005) and an excessive amount of nitric oxide (NO) is secreted by the primary defence system (Gilbert, 2012;Pande et al, 2013). Therefore, NO a by-product of L-arginine (Ozcan and Ogun, 2015) increases in both the uterine discharge and the blood serum (Xue et al, 2015).…”

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confidence: 99%

The Impact of Endometritis on Specific Oxidative Stress Parameters in Cows

Kaya

Öğün

Özen

et al. 2018

J Hellenic Vet Med Soc

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ABSTRACT. This study was aimed to investigate the presence of uterine infection and the relationship between the severity of uterine infection and some oxidative stress parameters in cows in early postpartum period. Sixty six cows that were in the period between the 30th and 32rd days of postpartum were included in the study. Endometrial inflammation was scored according to the condition of the uterus at rectal and ultrasonographic examinations and the character and amount of uterine discharge from endometritis I through III, the latest being the most severe. Endometrial samples were taken from the clinically healthy animals by cytobrush method, and then stained with Giemsa for presence of polymorphonuclear leukocytes (PMN). Cows with a PMN percentage less than 18% were considered to be healthy (control, n=20). All cows were sampled for blood. Nitric oxide (NO) levels in Group I (Endometritis I; n=20), Group II (Endometritis II; n=16), Group III (Endometritis III; n=10) and the control group were determined to be 23.0±0.63 nmol/mL, 32.23±0.97 nmol/mL, 36.56±0.48 nmol/mL, and 11.10±0.29 nmol/mL, respectively. The differences among the groups were found to be statistically significant. Malondialdehyde (MDA) level was highest in Group III (6.38±0.13 μmol/L), and its level significantly decreased with the reduced severity of endometritis. The comparison of the groups for total antioxidant capacity (TAC) and total oxidant capacity (TOC) demonstrated that endometritis caused a decrease in TAC levels (P<0.05), but did not affect TOC levels. Endometritis increased serum NO and MDA levels, and decreased TAC levels, but had no effect on TOC levels. In conclusion, NO and MDA, as well as TAC can be used as a biochemical marker for estimation of the severity of endometritis in cows.

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Changes in malondialdehyde concentrations and glutathione peroxidase activity in purebred Arabian mares with endometritis

Cited by 19 publications

References 17 publications

Oxidant/Antioxidant Status Assessment of Blood in Selected Equine Diseases

Oxidant/Antioxidant Status Assessment of Blood in Selected Equine Diseases

The effect of preoperative antibiotic or antibiotic + vitamin C administrations on the inflammatory and oxidative state in the rabbits with experimentally induced pyometra

The Impact of Endometritis on Specific Oxidative Stress Parameters in Cows

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