Changes in Lymphocyte Phenotypes and Cytokine Production by Surgical Stress in a Rat Small Intestinal Resection Model

Murakami, Mitsukiyo; Sato, Naoko; Sato, Natsufumi; Nakamura, Tsuyoshi; Masunaga, Hiroaki

doi:10.3164/jcbn.40.216

Cited by 17 publications

(15 citation statements)

References 20 publications

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“…Our results revealed a predominant pro-inflammatory and Th1 systemic response at the third day after intestinal resection and anastomosis and a prevailing anti-inflammatory and Th2 systemic reaction at the seventh day, in concordance with the literature 29 . As reported by previous experimental and clinical studies, the initial pro-inflammatory phase of the host response during the early postoperative period is followed by anti-inflammatory cytokines production by Th2 lymphocytes 30 .…”

Section: ■ Discussionsupporting

confidence: 80%

“…As reported by previous experimental and clinical studies, the initial pro-inflammatory phase of the host response during the early postoperative period is followed by anti-inflammatory cytokines production by Th2 lymphocytes 30 . Surgical stress induces a shift in the Th1/Th2 cell balance towards Th2 22,29,31 , suggesting downregulation of cell-mediated and upregulation of antibodymediated immunity, proportional to the magnitude of injury 22,31 . This study demonstrated a favorable influence of teduglutide on intestinal cellular viability in the perioperative context, particularly after ileal resection and anastomosis, and confirms its anti-apoptotic effects.…”

Section: ■ Discussionmentioning

confidence: 99%

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Intestinal inflammatory and redox responses to the perioperative administration of teduglutide in rats

et al. 2017

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1 AbstractPurpose: To investigate the inflammatory and redox responses to teduglutide on an animal model of laparotomy and intestinal anastomosis. Methods: Wistar rats (n=62) were allocated into four groups: "Ileal Resection and Anastomosis" vs. "Laparotomy", each one split into "Postoperative Teduglutide Administration" vs. "No Treatment"; and euthanized at the third or the seventh day. Ileal and blood samples were recovered at the baseline and at the euthanasia. Flow cytometry was used to study the inflammatory response (IL-1α, MCP-1, TNF-α, IFN-γ and IL-4 levels), oxidative stress (cytosolic peroxides, mitochondrial reactive species, intracellular glutathione and mitochondrial membrane potential) and cellular viability and death (annexin V/propidium iodide double staining). Results: Postoperative teduglutide treatment was associated with higher cellular viability index and lower early apoptosis ratio at the seventh day; higher cytosolic peroxides level at the third day and mitochondrial overgeneration of reactive species at the seventh day; higher tissue concentration of IL-4 and lower local pro-to-anti-inflammatory cytokines ratio at the seventh day. Conclusion: Those findings suggest an intestinal pro-oxidative and anti-inflammatory influence of teduglutide on the peri-operative context with a potential interference in the intestinal anastomotic healing.

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Section: ■ Discussionsupporting

confidence: 80%

Section: ■ Discussionmentioning

confidence: 99%

Intestinal inflammatory and redox responses to the perioperative administration of teduglutide in rats

et al. 2017

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“… 93 In addition, rodent studies have shown that massive SBR results in a reduction of the lymphocyte subpopulation including CD4+ and CD8+ T cells, as well as B cells in blood, mesenteric lymph nodes, and spleen. 110 , 111 The decrease in systemic immunity also was associated with increased levels of the proinflammatory cytokine IL6 in the plasma at day 5 after surgery. 110 …”

Section: Microbiota and Host Immune System Cross-talk During Intestinmentioning

confidence: 96%

“… 110 , 111 The decrease in systemic immunity also was associated with increased levels of the proinflammatory cytokine IL6 in the plasma at day 5 after surgery. 110 …”

Section: Microbiota and Host Immune System Cross-talk During Intestinmentioning

confidence: 96%

Host-Gut Microbiota Crosstalk in Intestinal Adaptation

Marchix

Goddard

Helmrath

2018

Cellular and Molecular Gastroenterology and Hepatology

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Short-bowel syndrome represents the most common cause of intestinal failure and occurs when the remaining intestine cannot support fluid and nutrient needs to sustain adequate physiology and development without the use of supplemental parenteral nutrition. After intestinal loss or damage, the remnant bowel undergoes multifactorial compensatory processes, termed adaptation, which are largely driven by intraluminal nutrient exposure. Previous studies have provided insight into the biological processes and mediators after resection, however, there still remains a gap in the knowledge of more comprehensive mechanisms that drive the adaptive responses in these patients. Recent data support the microbiota as a key mediator of gut homeostasis and a potential driver of metabolism and immunomodulation after intestinal loss. In this review, we summarize the emerging ideas related to host-microbiota interactions in the intestinal adaptation processes.

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“…Barrena et al reported systemic immune alterations, such as reduction in CD4 + lymphocytes and B lymphocytes, in SBS animal model 7 days after surgery [12]. It may however re ect surgical stress rather than immunological imbalance caused by bowel removal, especially that analogical disturbances were also observed after other surgical interventions [13]. Another study demonstrated changes in circulating lymphocytes numbers also 3 weeks after surgery [14].…”

Section: Discussionmentioning

confidence: 99%

Does massive bowel resection in newborns affect further immunity in children ?

Sznurkowska¹,

Borkowska²,

Jankowska³

et al. 2020

Preprint

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Background Short bowel syndrome (SBS) is defined as the a malabsorptive condition most often caused by massive resection of the small intestine. In children most cases of SBS originate in the newborn period and result from congenital anomalies or necrotizing enterocolitis. Loss of gut mucosa during resection does not only mean loss of absorption surface, but also deprives organism of many immunocompetent cells concentrated in gut associated lymphoid tissue, which is regarded the largest immune organ in humans. Aim of the study: We have aimed to access the influence of bowel resection on adaptive immunity in children, basing on peripheral lymphocyte populations and serum immunoglobulins. Patients and methods: 18 children, who underwent bowel resection in the first month of life and required further home parenteral nutrition were enrolled into the study. 12 healthy children, constituted control group. Based on flow cytometry the following subpopulations of lymphocytes were evaluated: T, B, NK, CD4+, C8 + and activated T cells. Serum immunoglobulins were determined with the use of immunoturbidimetric method. Results The percentage of B lymphocytes was reduced, while the rates of lymphocytes T and CD8 + lymphocytes were higher compared to healthy children. We documented significantly lower absolute count and proportion of NK cells in SBS group than in the control group. Absolute counts of lymphocytes, lymphocytes B, T, CD4 + and percentages of lymphocytes CD4+, and activated T cells inversely correlated with the time after resection. No statistically significant differences were found between the levels of IgA, IgM and IgG in the studied and the control group Conclusions Children with SBS do not present with clinical signs of immunodeficiency as well as deficits in peripheral lymphocyte populations and serum immunoglobulins. Lower number of NK cells in SBS patients compared to healthy children needs to be verified in larger cohort. The tendency of the lymphocyte subpopulations to decrease over time after resection points out the necessity for longer follow- up.

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Changes in Lymphocyte Phenotypes and Cytokine Production by Surgical Stress in a Rat Small Intestinal Resection Model

Cited by 17 publications

References 20 publications

Intestinal inflammatory and redox responses to the perioperative administration of teduglutide in rats

Intestinal inflammatory and redox responses to the perioperative administration of teduglutide in rats

Host-Gut Microbiota Crosstalk in Intestinal Adaptation

Does massive bowel resection in newborns affect further immunity in children ?

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