Changes in Localization of T Cell Subsets in the Ovarian Follicles during Follicular Growth and Ovulation in Hens

Subedi, Kalpana; Yoshimura, Y.

doi:10.2141/jpsa.42.215

Cited by 2 publications

(1 citation statement)

References 17 publications

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“…RT-PCR to identify MHC class II was performed as described previously [29]. Total RNAs were extracted from the right-side testis, epididymis, ductus deferens, and spleen of 550-day-old birds (n =4) using Sepasol RNA I Super (Nacalai Tesque, Inc., Kyoto, Japan) according to the manufacturer's protocol.…”

Section: Rt-pcrmentioning

confidence: 99%

Effects of Age and Gonadal Steroids on the Localization of Antigen Presenting Cells in the Epididymis of the Male Chicken, Gallus domesticus

Yoshimura

Fukui

Nishibori

et al. 2006

Journal of Reproduction and Development

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Abstract. The goal of this study was to localize antigen presenting cells (APC), which may play roles in defense against pathogens and fertility, and examine the effects of age and gonadal steroids on their population in the rooster epididymis. Healthy White Leghorn male birds (immature 60-day-old birds; matured 150-, 330-, and 550-day-old), and immature birds treated with testosterone propionate (TP) or estradiol benzoate (EB) for 3 or 6 days were used. Cryostat sections of the epididymis and ductus deference were immunostained for Ia to identify APC. RT-PCR was performed to confirm the expression of major histocompatibility complex class II (MHC class II) mRNA in the epididymis. Ia + cells were localized in the surface epithelium and subepithelial layer of the ductules and occasionally in the luminal content of the epididymis and ductus deference. RT-PCR analysis confirmed expression of MHC class II mRNA in the epididymis, ductus deferens, testis, and spleen. The frequency of Ia + cells in the subepithelial layer was significantly greater in the proximal efferent ductules than in the other two types of ductules in the epididymis of 550-day-old birds. Although there were no significant differences in the frequencies in the subepithelial layer of the proximal efferent ductules between 60-and 150-day-old birds, the frequencies were significantly greater in 330-and 550-day-old birds than in 60-day-old birds. The frequencies of Ia + cells in the ductus deferences was increased in the 150-day-old birds compared with the 60-day-old birds, with a larger increase in 330-and 550-day-old birds. The Ia + cell frequency was significantly increased by EB-injection, but not by TP-injection, on Days 3 and 6 of injection compared with Day 0. These results suggest that the population of APC in the epididymis increases with age after sexual maturation, and estrogen may be one of the factors involved in induction of Ia + cells.

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Section: Rt-pcrmentioning

confidence: 99%

Effects of Age and Gonadal Steroids on the Localization of Antigen Presenting Cells in the Epididymis of the Male Chicken, Gallus domesticus

Yoshimura

Fukui

Nishibori

et al. 2006

Journal of Reproduction and Development

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Changes in the Localization of Immunoreactive Avian Beta-Defensin-8, -10 and -12 in Hen Ovarian Follicles during Follicular Growth

2010

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Avian beta-defensins (avBDs) play significant roles in the innate immune system. The aim of this study was to identify immunoreactive (ir) avBDs proteins in the hen ovarian follicles and the changes in their localization with follicular growth. The ovarian follicles at di erent growth stages, namely the largest (F ), second and third largest (F and F ), prehierarchal small yellow and cortical follicles, were collected. The presence of ir-avBD-, -, and -were examined by immunohistochemistry and Western blot. The three ir-avBDs showed a similar pattern of immunostainings in the follicular tissues at di erent growth stages. In the granulosa cells, the ir-avBDs were identified in the cortical follicles, whereas their density was reduced in small yellow follicles. The granulosa cells of yellow follicles (F -F ) showed dense immunolabelings. The interstitial cells showed a faint immunolabeling for avBD-but not for avBD-and -in the cortical follicles, whereas they were weakly stained in the small yellow follicles. Dense immunoreaction products were noticed in the theca interna cells of F -F follicles. Western blot analysis showed a single band for each defensin. These results suggest that avBD-, -andproteins are expressed in the specific cells in the follicles, namely interstitial or theca interna cells and granulosa cells, where their amounts are likely increased with follicular growth. These avBDs may play significant roles in the host innate immune system in the follicles.

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Changes in Localization of T Cell Subsets in the Ovarian Follicles during Follicular Growth and Ovulation in Hens

Cited by 2 publications

References 17 publications

Effects of Age and Gonadal Steroids on the Localization of Antigen Presenting Cells in the Epididymis of the Male Chicken, Gallus domesticus

Effects of Age and Gonadal Steroids on the Localization of Antigen Presenting Cells in the Epididymis of the Male Chicken, Gallus domesticus

Changes in the Localization of Immunoreactive Avian Beta-Defensin-8, -10 and -12 in Hen Ovarian Follicles during Follicular Growth

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