Changes in lipids during matrix: Induced endochondral bone formation

Abstract

The changes in lipids occurring during the process of endochondral ossification have been characterized by studying the discrete phases of matrix-induced endochondral bone formation in the rat. Calcium-acidic phospholipid-phosphate complexes were shown to increase in concentration during cartilage calcification (day 9) and to peak in content during early bone formation (days 11-13), the times during which the rate of mineral deposition, as indicated by the change in ash weight was greatest. These data support the hypothesis that the calcium-acidic phospholipid-phosphate complexes play a role in the in vivo initiation of hydroxyapatite deposition. The overall lipid composition of the induced matrix newly formed cartilage (days 7-9) was comparable to that of normal cartilage, with the phospholipid composition matching that of chondrocyte plasma membranes. Times of vascular invasion and formation of marrow cavities were marked by elevated total lipid and triglyceride contents.

Aging Versus Postmenopausal Osteoporosis: Bone Composition and Maturation Kinetics at Actively-Forming Trabecular Surfaces of Female Subjects Aged 1 to 84 Years

“…In health, the highest lipid content was evident in the AD group (Tables 1 and 4), in agreement with their suggested biological role (35,36) as biomineralization nucleators, because this is the subject group in which the highest bone mass is usually encountered.…”

“…(35,36) 6. The relative pyridinoline content was calculated from the ratio of the Raman intensity at 1660 cm…”

Pediatric reference Raman data for material characteristics of iliac trabecular bone

“…5) Relative lipids content (normalized to organic matrix); lipids have been implicated in the literature as mineral nucleators, responsible for mineralization of collagen fibers. A layer of round lipid particles has been identified to mediate collagen calcification in compact bone formation, and calcium-acidic phospholipid-phosphate complexes have been shown to increase in concentration during cartilage calcification and early bone formation [72][73][74][75]. In the present study, the relative lipid content was significantly dependent on tissue age, with the highest values encountered in youngest bone formed, in agreement with what has been reported in the literature concerning their role.…”

Mechanism of Mineralization of Collagen‐Based Connective Tissues

“…These complexes were also found to be present in lesser amounts in diseases where mineralization was impaired, and to increase concomitant with mineralization [219][220][221].…”