Changes in lipid peroxidation in stay-green leaves of tobacco with senescence-induced synthesis of cytokinins

Pilarska, Maria; Skowron, Ernest; Pietraś, Rafał; Krupinska, Karin; Niewiadomska, Ewa

doi:10.1016/j.plaphy.2017.06.018

Cited by 11 publications

(7 citation statements)

References 35 publications

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“…The most abundant final product of ROS-triggered lipid peroxidation is MDA (Pilarska et al 2017) and its accumulation under drought was related to the SOD activity and endogenous H 2 O 2 level as shown within the CT and non-sprayed (D) sample (Figs. 5a-c).…”

Section: Discussionmentioning

confidence: 82%

Effect of exogenously-applied abscisic acid, putrescine and hydrogen peroxide on drought tolerance of barley

Skowron

Trojak

2020

Biologia

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The objective of this study was to identify the effect of abscisic acid (ABA), putrescine (Put) and hydrogen peroxide (H2O2) foliar pre-treatment on drought tolerance of barley. Despite water limitation, ABA-sprayed plants preserved increased water content, photosynthetic efficiency of PSII (ΦPSII) and CO2 assimilation rate (Pn) compared to untreated stressed plants. The ABA-treated plants presented also the lowest rate of lipid peroxidation (MDA), lowered the rate of PSII primary acceptor reduction (1 – qP) and increased the yield of regulated energy dissipation (NPQ) with higher accumulation of PGRL1 (PROTON GRADIENT REGULATION LIKE1) protein. These plants preserved a similar level of photochemical efficiency and the rate of electron transport of PSII (ETRII) to the well-watered samples. The significantly less pronounced response was observed in Put-sprayed samples under drought. Additionally, the combined effects of drought and H2O2 application increased the 1 – qP and quantum yield of non-regulated energy dissipation in PSII (ΦNO) and reduced the accumulation of Rubisco activase (RCA). In conclusion, ABA foliar application allowed to balance water retention and preserve antioxidant capacity resulting in efficient photosynthesis and the restricted risk of oxidative damage under drought. Neither hydrogen peroxide nor putrescine has been able to ameliorate drought stress as effectively as ABA.

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Section: Discussionmentioning

confidence: 82%

Effect of exogenously-applied abscisic acid, putrescine and hydrogen peroxide on drought tolerance of barley

Skowron

Trojak

2020

Biologia

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“…Recently, leaves of pSAG12::IPT overexpressing tobacco plants were allowed to undergo natural senescence and lipid peroxidation rate was determined by GS-MS using end product malondialdehyde (MDA). Though leaves of pSAG12::IPT remained green due to delayed senescence, lipid peroxidation was much higher compared to WT leaves of the same age [112]. Results indicated that lipid peroxidation cannot be correlated with leaf senescence.…”

Section: Cytokinins Moderate Ros Levels During Osmotic Stressesmentioning

confidence: 91%

The Mode of Cytokinin Functions Assisting Plant Adaptations to Osmotic Stresses

Gujjar

Supaibulwatana

2019

Plants

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Plants respond to abiotic stresses by activating a specific genetic program that supports survival by developing robust adaptive mechanisms. This leads to accelerated senescence and reduced growth, resulting in negative agro-economic impacts on crop productivity. Cytokinins (CKs) customarily regulate various biological processes in plants, including growth and development. In recent years, cytokinins have been implicated in adaptations to osmotic stresses with improved plant growth and yield. Endogenous CK content under osmotic stresses can be enhanced either by transforming plants with a bacterial isopentenyl transferase (IPT) gene under the control of a stress inducible promoter or by exogenous application of synthetic CKs. CKs counteract osmotic stress-induced premature senescence by redistributing soluble sugars and inhibiting the expression of senescence-associated genes. Elevated CK contents under osmotic stress antagonize abscisic acid (ABA) signaling and ABA mediated responses, delay leaf senescence, reduce reactive oxygen species (ROS) damage and lipid peroxidation, improve plant growth, and ameliorate osmotic stress adaptability in plants.

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“…Moreover, the increased levels of H 2 O 2 resulted into elevated lipid peroxidation [36]. It was shown by the analysis of the end-product of lipid peroxidation, malondialdehyde (MDA), that lipid peroxidation is not associated with leaf senescence in stay-green tobacco plants [14].…”

Section: Antisenescent and Antioxidant Activity Of Natural Cytokinmentioning

confidence: 99%

“…Many of the biological activities of CKs in plants can be explained by their involvement in cellular oxidative stress, and the antioxidant capacity of these molecules has already been described [12]. Leaf senescence is accompanied by a gradual decline in antioxidants and an increase in reactive oxygen species (ROS) and content of malondialdehyde (a decomposition product of lipid peroxidation) in certain plants [13,14]. Increased lipid peroxidation and H 2 O 2 formation have been demonstrated along with a decline in the activities of enzymes, such as catalase (CAT) and ascorbate peroxidase (APX), as well as glutathione (GSH) content during senescence in pea and Arabidopsis leaves [13,15].…”

Section: Introductionmentioning

confidence: 99%

Role of Cytokinins in Senescence, Antioxidant Defence and Photosynthesis

Hönig

Plíhalová

Husičková

et al. 2018

IJMS

158

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Cytokinins modulate a number of important developmental processes, including the last phase of leaf development, known as senescence, which is associated with chlorophyll breakdown, photosynthetic apparatus disintegration and oxidative damage. There is ample evidence that cytokinins can slow down all these senescence-accompanying changes. Here, we review relationships between the various mechanisms of action of these regulatory molecules. We highlight their connection to photosynthesis, the pivotal process that generates assimilates, however may also lead to oxidative damage. Thus, we also focus on cytokinin induction of protective responses against oxidative damage. Activation of antioxidative enzymes in senescing tissues is described as well as changes in the levels of naturally occurring antioxidative compounds, such as phenolic acids and flavonoids, in plant explants. The main goal of this review is to show how the biological activities of cytokinins may be related to their chemical structure. New links between molecular aspects of natural cytokinins and their synthetic derivatives with antisenescent properties are described. Structural motifs in cytokinin molecules that may explain why these molecules play such a significant regulatory role are outlined.

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Changes in lipid peroxidation in stay-green leaves of tobacco with senescence-induced synthesis of cytokinins

Cited by 11 publications

References 35 publications

Effect of exogenously-applied abscisic acid, putrescine and hydrogen peroxide on drought tolerance of barley

Effect of exogenously-applied abscisic acid, putrescine and hydrogen peroxide on drought tolerance of barley

The Mode of Cytokinin Functions Assisting Plant Adaptations to Osmotic Stresses

Role of Cytokinins in Senescence, Antioxidant Defence and Photosynthesis

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