Changes in lipid composition of the maturing rat testis

Abstract

1. The lipids and fatty acids of the lipids of testes of rats aged 4 weeks to 6 months were separated and analysed. 2. A decrease in concentration of triglyceride was noted, but there was no significant change in the concentration of phospholipids, plasmalogen or cholesterol during this time. 3. There were no significant differences in the total lipid concentration of palmitic acid, stearic acid, linoleic acid, arachidonic acid and docosatetraenoic acid between the various age groups. 4. A decrease in the concentration of oleic acid in the phosphatide and triglyceride fractions and an increase in the concentration of docosapentaenoic acid (characterized as the Delta4,7,10,13,16-isomer) in phosphatides but not in triglyceride were observed during the maturation period. 5. Histological studies indicated that the lipid changes occurred at the same time as the appearance and maturation of the spermatids.

Retinal very long-chain PUFAs: new insights from studies on ELOVL4 protein

“…The presence of VLC-PUFA in the mammalian testis was fi rst reported in the late 1960s (46)(47)(48)(49)(50). Later, Grogan et al ( 51,52 ) reported that isolated spermatocytes and spermatids synthesized VLC-PUFA from 14 ( 53 ).…”

Differentiation-related changes in lipid classes with long-chain and very long-chain polyenoic fatty acids in rat spermatogenic cells

“…Whereas the amount of 22:5n-6 in GPL progressively increases in the testis with spermatogenic cell growth and differentiation ( 13 ), it falls with the involution the mature organ undergoes as it gradually loses its germ cells as a long-term consequence of damaging stem spermatogonia after treatment with a single dose of X-ray irradiation ( 10 ). In the present study, the main GPL classes and subclasses that contribute to these differentiation-related changes were analyzed in pachytene spermatocytes, round spermatids, and residual bodies to contrast them as germ cell products with the VLCPUFA-containing SM and Cer.…”

Mechanisms of aging in relationship to steroid biotransformations and the thiobarbituric acid reactive material in rate testicular tissue in vitro

“…According to Davis, Bridges, and Coniglio (1966), the cholesteryl esters and triglycerides decreased with the age of the animal, but Oshima and Carpenter (1968) reported a marked increase of these fractions with age. A significant characteristic of the testicular triglycerides from the adult animal was that they have a high content of unsaturated fatty acids.…”