Changes in leukocyte glutamate dehydrogenase activity in alcoholics upon break in alcohol consumption

Kravos, Matej; Malešič, Ivan

doi:10.1016/j.clinbiochem.2009.10.011

Cited by 4 publications

(4 citation statements)

References 21 publications

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“…The alcohol-induced activation of glutamate dehydrogenase, in particular in the liver and leukocytes, has been reported earlier (Cascales et al 1985; Kravos and Malesic 2010). The activity of mitochondrial glutamate dehydrogenase was shown to rise after both chronic ethanol and acetaldehyde treatment (Cascales et al 1985).…”

Section: Discussionsupporting

confidence: 58%

Glutamate contributes to alcohol hepatotoxicity by enhancing oxidative stress in mitochondria

Теплова

Kruglov

Kovalyov

et al. 2017

J Bioenerg Biomembr

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Chronic alcohol intoxication is associated with increased oxidative stress. However, the mechanisms by which ethanol triggers an increase in the production of reactive oxygen species (ROS) and the role of mitochondria in the development of oxidative stress has been insufficiently studied. The biochemical and proteomic data obtained in the present work suggest that one of the main causes of an increase in ROS generation is enhanced oxidation of glutamate in response to long-term alcohol exposure. In the course of glutamate oxidation, liver mitochondria from alcoholic rats generated more superoxide anion and H2O2 than in the presence of other substrates and more than control organelles. In mitochondria from alcoholic rats, rates of H2O2 production and NAD reduction in the presence of glutamate were almost twice higher than in the control. The proteomic study revealed a higher content of glutamate dehydrogenase in liver mitochondria of rats subjected to chronic alcohol exposure. Simultaneously, the content of mitochondrial catalase decreased compared to control. Each of these factors stimulates the production of ROS in addition to ROS generated by the respiratory chain complex I. The results are consistent with the conclusion that glutamate contributes to alcohol hepatotoxicity by enhancing oxidative stress in mitochondria.

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Section: Discussionsupporting

confidence: 58%

Glutamate contributes to alcohol hepatotoxicity by enhancing oxidative stress in mitochondria

Теплова

Kruglov

Kovalyov

et al. 2017

J Bioenerg Biomembr

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“…The GLDH activity and the protein concentration in 4 samples of leukocyte suspension were determined 8 times in series (n = 8). The average variation coefficient of the GLDH catalytic activity in leukocytes (microkatals/gram) in the 4 samples came to 6.82% [16] .…”

Section: Methodsmentioning

confidence: 99%

Glutamate Dehydrogenase Activity in Leukocytes and Ageing

Kravos

Malešič²

2010

Neurodegener Dis

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Background: Glutamate dehydrogenase (GLDH) is an intracellular enzyme normally located in mitochondria and the rough endoplasmic reticulum. The metabolism of neuroexcitotoxic glutamate is lowered in many neurodegenerative disorders. Objective: Defining the GLDH activity in leukocytes can provide indirect data about neurodegenerative processes in the brain. Method: We have developed our own method of defining the GLDH activity in leukocytes. Results: The GLDH activity was researched in the leukocytes of 130 healthy subjects (45 females, 85 males). The mean catalytic activity was 0.618 ± 0.380 µkat/g. The highest values were discovered in <30-year-old persons: 0.772 ± 0.434 µkat/g (0.712 ± 0.406 µkat/g in men, 0.934 ± 0.5 µkat/g in women). The GLDH activity decreases more slowly in the 30- to 60-year age group, yet evidently more rapidly afterwards, particularly in men, where the activity drops to 0.333 µkat/g and in women to 0.414 µkat/g. The older persons had a lower leukocyte GLDH activity than the younger ones (ANOVA, p = 0.000). Conclusion: Gradual decrease in GLDH activity may be one of the key factors for neurodegenerative ageing processes.

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“…Its production is controlled by glutaminase (GLS), while glutamate dehydrogenase (GDH) and glutamate decarboxylase catalyze its degradation [17, 18]. …”

Section: Introductionmentioning

confidence: 99%

The Markers of Glutamate Metabolism in Peripheral Blood Mononuclear Cells and Neurological Complications in Lung Cancer Patients

et al. 2016

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Objective. To evaluate the involvement of glutamate metabolism in peripheral blood mononuclear cells (PBMC) in the development of neurological complications in lung cancer and during chemotherapy. Methods. The prospective study included 221 lung cancer patients treated with chemotherapeutics. Neurological status and cognitive functions were evaluated at baseline and after 6-month follow-up. Glutamate level, the activities of glutaminase- (GLS-) glutamate synthetizing enzyme, glutamate dehydrogenase (GDH), and glutamate decarboxylase catalyzing glutamate degradation were analyzed in PBMC and in sera of lung cancer patients by means of spectrophotometric and colorimetric methods. Results. Chemotherapy of lung neoplasms induced increase of glutamate content in PBMC and its concentration in serum increased the activity of GDH in PBMC and decreased activity of glutaminase in PBMC. The changes in glutamate metabolism markers were associated with initial manifestation of neurological deficit in lung cancer patients and with new symptoms, which appear as a complication of chemotherapy. Moreover, the analyzed parameters of glutamate control correlated with a spectrum of cognitive functions measures in lung cancer patients. Conclusion. We have demonstrated dysregulation in glutamate and glutamate metabolism controlling enzymes as promising indicators of risk for chemotherapy-induced neurological complications in lung cancer patients with particular emphasis on cognitive impairment.

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Changes in leukocyte glutamate dehydrogenase activity in alcoholics upon break in alcohol consumption

Cited by 4 publications

References 21 publications

Glutamate contributes to alcohol hepatotoxicity by enhancing oxidative stress in mitochondria

Glutamate contributes to alcohol hepatotoxicity by enhancing oxidative stress in mitochondria

Glutamate Dehydrogenase Activity in Leukocytes and Ageing

The Markers of Glutamate Metabolism in Peripheral Blood Mononuclear Cells and Neurological Complications in Lung Cancer Patients

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