Changes in Invertase Activities and Reducing Sugar Content in Sweetpotato Stored at Different Temperatures

Huang, Yuehe; Picha, David H.; Kilili, and A. W.; Johnson, Charles E.

doi:10.1021/jf9902191

Cited by 24 publications

(19 citation statements)

References 16 publications

(25 reference statements)

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“…In these transgenic plants, the contents of glucose and fructose in the storage roots were remarkably reduced, while that of sucrose remained largely unchanged. This result is consistent with the previous reports describing a positive correlation between acid invertase activity and hexose content in sweetpotato storage roots (Takahata et al 1996;Huang et al 1999) and also suggests that Ibbfruct2 plays important roles in determining the utilization of sugar in the storage roots. Previous studies have reported that the expression of Ibbfruct2 was observed both in young and mature storage roots, while the expression of the other two genes (Ibbfruct1 and Ibbfruct3) was weak or undetectable in those tissues (Li and Zhang 2003;Wang et al 2005).…”

Section: Discussionsupporting

confidence: 93%

Altered carbohydrate metabolism in the storage roots of sweetpotato plants overexpressing the SRF1 gene, which encodes a Dof zinc finger transcription factor

Tanaka

Takahata

Nakayama

et al. 2009

Planta

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In order to characterize the functions of the sweetpotato SRF1 gene, which encodes a Dof zinc finger transcriptional factor preferentially expressed in the storage roots, we isolated its full length cDNA and produced transgenic sweetpotato plants with altered SRF1 expression levels. The isolated cDNA of SRF1 encoded a polypeptide of 497 amino acids and was closely related to the cyclic Dof factors of Arabidopsis and the ascorbate oxidase binding protein of pumpkin. SRF1 was most highly expressed in storage roots, although some expression was also observed in other vegetative tissue. Transgenic plants overexpressing SRF1 showed significantly higher storage root dry matter content compared to the original cultivar Kokei No. 14 or control transgenic plants. In these plants, the starch content per fresh weight of the storage roots was also higher than that of the wild-type plants, while the glucose and fructose content drastically decreased. Among the enzymes involved in the sugar metabolism, soluble acid invertase showed a decreased activity in the transgenic plants. Gene expression analysis showed that the expression of Ibbetafruct2, which encodes an isoform of vacuolar invertase, was suppressed in the transgenic plants overexpressing the SRF1 gene. These data suggest that SRF1 modulates the carbohydrate metabolism in the storage roots through negative regulation of a vacuolar invertase gene.

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Section: Discussionsupporting

confidence: 93%

Altered carbohydrate metabolism in the storage roots of sweetpotato plants overexpressing the SRF1 gene, which encodes a Dof zinc finger transcription factor

Tanaka

Takahata

Nakayama

et al. 2009

Planta

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“…The enzyme invertase, also called sucrase, catalyzes the hydrolysis of sucrose to glucose and fructose (Huang et al 1999; Zhang et al 2016). Invertase is found in the potential urine contaminant yeast (Fisher et al 1995).…”

Section: Discussionmentioning

confidence: 99%

Stability of targeted metabolite profiles of urine samples under different storage conditions

et al. 2016

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IntroductionFew studies have investigated the influence of storage conditions on urine samples and none of them used targeted mass spectrometry (MS).ObjectivesWe investigated the stability of metabolite profiles in urine samples under different storage conditions using targeted metabolomics.MethodsPooled, fasting urine samples were collected and stored at −80 °C (biobank standard), −20 °C (freezer), 4 °C (fridge), ~9 °C (cool pack), and ~20 °C (room temperature) for 0, 2, 8 and 24 h. Metabolite concentrations were quantified with MS using the AbsoluteIDQ™ p150 assay. We used the Welch-Satterthwaite-test to compare the concentrations of each metabolite. Mixed effects linear regression was used to assess the influence of the interaction of storage time and temperature.ResultsThe concentrations of 63 investigated metabolites were stable at −20 and 4 °C for up to 24 h when compared to samples immediately stored at −80 °C. When stored at ~9 °C for 24 h, few amino acids (Arg, Val and Leu/Ile) significantly decreased by 40% in concentration (P < 7.9E−04); for an additional three metabolites (Ser, Met, Hexose H1) when stored at ~20 °C reduced up to 60% in concentrations. The concentrations of four more metabolites (Glu, Phe, Pro, and Thr) were found to be significantly influenced when considering the interaction between exposure time and temperature.ConclusionOur findings indicate that 78% of quantified metabolites were stable for all examined storage conditions. Particularly, some amino acid concentrations were sensitive to changes after prolonged storage at room temperature. Shipping or storing urine samples on cool packs or at room temperature for more than 8 h and multiple numbers of freeze and thaw cycles should be avoided.Electronic supplementary materialThe online version of this article (doi:10.1007/s11306-016-1137-z) contains supplementary material, which is available to authorized users.

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“…Various studies have examined the effects of different storage temperatures on sugar content in other plant products (Ding et al, 1998;Huang et al, 1999). During storage at cold temperatures, potato tubers accumulate free reducing sugars derived from the breakdown of starch to sucrose, which is then converted into glucose and fructose (Blenkinsop et al, 2004;McKenzie et al, 2013;Samotus et al, 1974).…”

Section: Discussionmentioning

confidence: 99%

“…The invertase transcript did not accumulate in potato tubers stored at 10°C, but in tubers stored at 1°C, the invertase transcript level increased markedly within 7 days (Zhou et al, 1999). Invertase activities and reducing sugar concentration also significantly increased in roots of sweet potato kept at a low temperature (Huang et al, 1999). Sucrose loss was shown not to be increased by cold storage in loquat fruit (Ding et al, 1998), while sucrose content was largely unaffected during cold storage in apple (Ackermann et al, 1992).…”

mentioning

confidence: 88%

Effects of Storage Temperature on Fruit Quality and Expression of Sucrose Phosphate Synthase and Acid Invertase Genes in Japanese Pear

et al. 2015

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Japanese pear (Pyrus pyrifolia Nakai) 'Gold Nijisseiki' fruit were stored for 28 days at 0, 4, 10, 15, or 22°C. The effects of storage temperature on sucrose metabolism and on the expression of genes for sucrosemetabolizing enzymes were investigated. Fruit firmness, skin color, and content of various sugars were affected by storage temperature. Fruit stored at 22°C underwent rapid fruit softening and skin color change. Fruit stored at 15 or 10°C had lower sucrose content and higher glucose and fructose content than those stored at 0, 4, or 22°C. To investigate whether sucrose loss is related to changes in the expression of sucrose-metabolizing enzymes (vacuolar acid invertase: AIV; and sucrose phosphate synthase: SPS), we examined the expression of 3 genes during storage, namely, those encoding AIV (PpAIV1 and PpAIV2) and SPS (PpSPS1). Storage at 10 and 15°C increased the expression of PpAIV2 within a week and that of PpAIV1 in 14 days, while storage at 0 and 4°C delayed increased expression of PpAIV1 and PpAIV2 until after 28 days. Changes in the gene expression of sucrose-metabolizing enzymes were followed by delayed responses in sugar content.

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Changes in Invertase Activities and Reducing Sugar Content in Sweetpotato Stored at Different Temperatures

Cited by 24 publications

References 16 publications

Altered carbohydrate metabolism in the storage roots of sweetpotato plants overexpressing the SRF1 gene, which encodes a Dof zinc finger transcription factor

Altered carbohydrate metabolism in the storage roots of sweetpotato plants overexpressing the SRF1 gene, which encodes a Dof zinc finger transcription factor

Stability of targeted metabolite profiles of urine samples under different storage conditions

Effects of Storage Temperature on Fruit Quality and Expression of Sucrose Phosphate Synthase and Acid Invertase Genes in Japanese Pear

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