Changes in intra-nuclear mechanics in response to DNA damaging agents revealed by time-domain Brillouin micro-spectroscopy

Liu, Liwang; Simón, Marina; Muggiolu, Giovanna; Vilotte, Florent; Antoine, M.; Caron, J.; Kantor, G.; Barberet, Philippe; Seznec, Hervé; Audoin, Bertrand

doi:10.1016/j.pacs.2022.100385

Cited by 9 publications

(7 citation statements)

References 59 publications

(85 reference statements)

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“…While reported measurements of the loss modulus, longitudinal viscosity, and loss tangent are more difficult to find in the literature, our values are close to previously reported values. For example, Liu et al reported intra-nuclear loss moduli of 0.3–1.5 GPa in osteosarcoma cells 50 , matching the ~ 0.4 GPa reported here. Our measurements of longitudinal viscosity (~ 0.008 Pa s) are quite similar to those reported by Mattana et al 60 , who measured a value of ~ 6 to 12 centipoise in a control population of NIH/3T3 cells, a value equal to 0.006–0.012 Pa s. Finally, our loss tangents are comparable, but slightly higher than those reported by Chan et al 48 in intact mouse ovaries, though this may be explained by a difference in tissue type.…”

Section: Discussionsupporting

confidence: 89%

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Comprehensive single-shot biophysical cytometry using simultaneous quantitative phase imaging and Brillouin spectroscopy

Steelman¹,

Coker²,

Sedelnikova³

et al. 2022

Sci Rep

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Single-cell analysis, or cytometry, is a ubiquitous tool in the biomedical sciences. Whereas most cytometers use fluorescent probes to ascertain the presence or absence of targeted molecules, biophysical parameters such as the cell density, refractive index, and viscosity are difficult to obtain. In this work, we combine two complementary techniques—quantitative phase imaging and Brillouin spectroscopy—into a label-free image cytometry platform capable of measuring more than a dozen biophysical properties of individual cells simultaneously. Using a geometric simplification linked to freshly plated cells, we can acquire the cellular diameter, volume, refractive index, mass density, non-aqueous mass, fluid volume, dry volume, the fractional water content of cells, both by mass and by volume, the Brillouin shift, Brillouin linewidth, longitudinal modulus, longitudinal viscosity, the loss modulus, and the loss tangent, all from a single acquisition, and with no assumptions of underlying parameters. Our methods are validated across three cell populations, including a control population of CHO-K1 cells, cells exposed to tubulin-disrupting nocodazole, and cells under hypoosmotic shock. Our system will unlock new avenues of research in biophysics, cell biology, and medicine.

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Section: Discussionsupporting

confidence: 89%

“…Finally, the loss tangent may be calculated 48 – 50 , demonstrating the relative ratio of viscous to elastic effects.…”

Section: Methodsmentioning

confidence: 99%

Comprehensive single-shot biophysical cytometry using simultaneous quantitative phase imaging and Brillouin spectroscopy

Steelman¹,

Coker²,

Sedelnikova³

et al. 2022

Sci Rep

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show abstract

“…Taking the quantification of mechanical properties further, if the mass density, ρ , is known, measured, or approximated, the longitudinal modulus of the specimen can be determined. The complex longitudinal modulus ( M * ) describes both the storage and dissipation of energy during a uni-axial compression, and can be written as 5 , 39 , 40 Thus in addition to measuring the frequency of the signal, measuring the depth attenuation coefficient, α , of the phonon wavefront - either through fitting a decaying exponential to the time-domain signal 20 or measuring the frequency bandwidth of the TRBS ToF signal - provides access to the longitudinal loss modulus M ″ which describes energy dissipated during the compression.…”

Section: Methodsmentioning

confidence: 99%

Label-free Brillouin endo-microscopy for the quantitative 3D imaging of sub-micrometre biology

La Cavera,

Chauhan,

Hardiman

et al. 2024

Commun Biol

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This report presents an optical fibre-based endo-microscopic imaging tool that simultaneously measures the topographic profile and 3D viscoelastic properties of biological specimens through the phenomenon of time-resolved Brillouin scattering. This uses the intrinsic viscoelasticity of the specimen as a contrast mechanism without fluorescent tags or photoacoustic contrast mechanisms. We demonstrate 2 μm lateral resolution and 320 nm axial resolution for the 3D imaging of biological cells and Caenorhabditis elegans larvae. This has enabled the first ever 3D stiffness imaging and characterisation of the C. elegans larva cuticle in-situ. A label-free, subcellular resolution, and endoscopic compatible technique that reveals structural biologically-relevant material properties of tissue could pave the way toward in-vivo elasticity-based diagnostics down to the single cell level.

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“…The possibility of measuring the storage and loss moduli inside a cell nucleus is an advance offered recently by PU microscopy. It gives access to the nucleus nanostructure and compaction and rigidity of chromatin fibers, composed mainly of histones and DNA [156] . After DNA damage was induced in osteosarcoma nuclei by either chemical or physical means, the measurement of the complex moduli of the nucleus interior was achieved.…”

Section: Applications To the Nanoscale Investigation Of Cell Mechanicsmentioning

confidence: 99%

Changes in intra-nuclear mechanics in response to DNA damaging agents revealed by time-domain Brillouin micro-spectroscopy

Cited by 9 publications

References 59 publications

Comprehensive single-shot biophysical cytometry using simultaneous quantitative phase imaging and Brillouin spectroscopy

Comprehensive single-shot biophysical cytometry using simultaneous quantitative phase imaging and Brillouin spectroscopy

Label-free Brillouin endo-microscopy for the quantitative 3D imaging of sub-micrometre biology

Principles and advances in ultrafast photoacoustics; applications to imaging cell mechanics and to probing cell nanostructure

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