Changes in intestinal microbiota of Bufo gargarizans and its association with body weight during metamorphosis

Chai, Lihong; Dong, Zhibao; Chen, Aixia; Wang, Hongyuan

doi:10.1007/s00203-018-1523-1

Cited by 39 publications

(42 citation statements)

References 48 publications

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“…At the same time, many frog species complete the dietary shift during metamorphosis: from a plant material-based diet in tadpoles to primarily being insectivorous in adulthood (Jenssen, 1967;Linzey, 1967;Hendricks, 1973;Hourdry et al, 1996;Kupferberg, 1997;Castaneda et al, 2006). Several studies have investigated gut microbiota changes during metamorphosis in frogs [leopard frog Lithobates pipiens (Kohl et al, 2013), Bufo gargarizans (Chai et al, 2018), Lithobates [Rana] sylvaticus (Warne et al, 2017;Warne et al, 2019), Lithobates clamitans (Warne et al, 2017), and Lithobates catesbeianus (Warne et al, 2017)]. Kohl et al (2013) found a significant difference (decreased Proteobacteria and increased Firmicutes) in the gut microbial community between tadpoles and frogs (mature) and suggested that measurements at various time points throughout metamorphosis will provide better insight into detailed gut microbial dynamics (Kohl et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…Kohl et al (2013) found a significant difference (decreased Proteobacteria and increased Firmicutes) in the gut microbial community between tadpoles and frogs (mature) and suggested that measurements at various time points throughout metamorphosis will provide better insight into detailed gut microbial dynamics (Kohl et al, 2013). Chai et al (2018) found shifts in microbial composition (e.g., a reduction in Proteobacteria and Actinobacteria) among five developmental stages from aquatic larvae to terrestrial juveniles (frog), but not mature adults (Chai et al, 2018). In their study, there were no significant changes in the relative abundance of Firmicutes compared to the previous research (Kohl et al, 2013), which may be caused by the different stages and species examined (Chai et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Chai et al (2018) found shifts in microbial composition (e.g., a reduction in Proteobacteria and Actinobacteria) among five developmental stages from aquatic larvae to terrestrial juveniles (frog), but not mature adults (Chai et al, 2018). In their study, there were no significant changes in the relative abundance of Firmicutes compared to the previous research (Kohl et al, 2013), which may be caused by the different stages and species examined (Chai et al, 2018). These interesting studies reconstruct the gut microbial community at the composition level and provide information on the potential mechanism of gut microbiome-frog interactions during metamorphosis.…”

Section: Introductionmentioning

confidence: 99%

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The Changes in the Frog Gut Microbiome and Its Putative Oxygen-Related Phenotypes Accompanying the Development of Gastrointestinal Complexity and Dietary Shift

Zhang

Chen²,

Liu

et al. 2020

Front. Microbiol.

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There are many examples of symbiotic and reciprocal relationships in ecological systems; animal gut microbiome-host interactions are one such kind of bidirectional and complex relationship. Here, we utilized several approaches (16S rRNA gene sequencing, metagenomics, and transcriptomics) to explore potential gut microbiomehost interactions accompanying the development of gastrointestinal complexity and a dietary shift from metamorphosis to maturity in ornamented pygmy frogs (Microhyla fissipes). We identified the possible coevolution between a particular gut microbial group (increased putative fat-digesting Erysipelotrichaceae and chitin-digesting Bacteroides and Ruminococcaceae) and the host dietary shift [from herbivore to insectivore (high proportion of dietary chitin and fat)] during metamorphosis. We also found that the remodeling and complexity of the gastrointestinal system during metamorphosis might have a profound effect on the gut microbial community (decreasing facultative anaerobic Proteobacteria and increasing anaerobic Firmicutes) and its putative oxygen-related phenotypes. Moreover, a high proportion of chitin-digesting bacteria and increased carbohydrate metabolism by gut microbiomes at the climax of metamorphosis would help the frog's nutrition and energy needs during metamorphosis and development.Considering the increased expression of particular host genes (e.g., chitinase) in juvenile frogs, we speculate that host plays an important role in amphibian metamorphosis, and their symbiotic gut microbiome may help in this process by providing the nutrition and energy needs. We provide this basic information for the amphibian conservation and managements.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Changes in the Frog Gut Microbiome and Its Putative Oxygen-Related Phenotypes Accompanying the Development of Gastrointestinal Complexity and Dietary Shift

Zhang

Chen²,

Liu

et al. 2020

Front. Microbiol.

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“…The intestinal microbiota is a complex ecosystem and over 1000 human species have been described, mainly including Firmicutes , Bacteroidetes , Actinomycetes , Proteophylum and Verrucomicrobia (Chai et al . 2018). Once the intestinal microbiota is maladjusted, the development is facilitated of a variety of intestinal diseases, such as inflammatory bowel disease (IBD), primary sclerosing cholangitis (PSC)‐IBD, irritable bowel syndrome (IBS), chronic constipation (CC), osmotic diarrhoea and colorectal cancer.…”

Section: Introductionmentioning

confidence: 99%

Gut dysbacteriosis and intestinal disease: mechanism and treatment

Meng

Zhang

Cao³

et al. 2020

J. Appl. Microbiol.

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Summary The gut microbiome functions like an endocrine organ, generating bioactive metabolites, enzymes or small molecules that can impact host physiology. Gut dysbacteriosis is associated with many intestinal diseases including (but not limited to) inflammatory bowel disease, primary sclerosing cholangitis‐IBD, irritable bowel syndrome, chronic constipation, osmotic diarrhoea and colorectal cancer. The potential pathogenic mechanism of gut dysbacteriosis associated with intestinal diseases includes the alteration of composition of gut microbiota as well as the gut microbiota–derived signalling molecules. The many correlations between the latter and the susceptibility for intestinal diseases has placed a spotlight on the gut microbiome as a potential novel target for therapeutics. Currently, faecal microbial transplantation, dietary interventions, use of probiotics, prebiotics and drugs are the major therapeutic tools utilized to impact dysbacteriosis and associated intestinal diseases. In this review, we systematically summarized the role of intestinal microbiome in the occurrence and development of intestinal diseases. The potential mechanism of the complex interplay between gut dysbacteriosis and intestinal diseases, and the treatment methods are also highlighted.

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“…99Thus, amphibian metamorphosis is a post-embryonic developmental step during which host-100 microbial interactions can play critical roles. 101Changes in gut bacterial communities during anuran metamorphosis was first studied using 102 culturing methods and more recently using 16S rDNA gene metabarcoding (Fedewa, 2006; 103 Kohl et al, 2013;Vences et al, 2016;Chai et al, 2018;Warne et al, 2019;Long et al, 2020; 104 Zhang et al, 2020). A single study expanded its analysis using metagenomic and 105 metatranscriptomic (Zhang et al, 2020).…”

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confidence: 99%

Gut microbial ecology of Xenopus tadpoles across life stages

Scalvenzi

Clavereau

Bourge

et al. 2020

Preprint

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350 words max) 18 Background: The microorganism world living in amphibians is still largely under-represented 19and under-studied in the literature. Among anuran amphibians, African clawed frogs of the 20 Xenopus genus stand as well-characterized models with an in-depth knowledge of their 21 developmental biological processes including their metamorphosis. We used different 22 approaches including flow cytometry, 16s rDNA gene metabarcoding, metagenomic and 23 metatranscriptomic to analyze the succession of microbial communities and their activities 24 across different body habitats of Xenopus tropicalis. 25Results: We analyzed the bacterial components of the Xenopus gut microbiota, the adult gut 26 biogeography, the succession of communities during ontogeny, the impact of the alimentation 27 in shaping the tadpole's gut bacterial communities, the transmission of skin and fecal bacteria 28 to the eggs. We also identified the most active gut bacteria and their metabolic contribution to 29 the tadpole physiology and showed the close resemblance between amphibian and mammalian 30 gut microbiomes. 31Our results show that in the growing tadpole, the same predominant bacterial phylum as in the 32 mammalian gut is present as a large fraction of the 82,679 genes identified in the Xenopus 33 tadpole's gut metagenome encode proteins sharing common functions with proteins found in 34 the human gut microbiome. 35 Conclusions:We present a comprehensive new microbiome dataset of a laboratory amphibian 36 model. Our data provide evidences that studies on the Xenopus tadpole model can shed light on 37 the interactions between a vertebrate host and its microbiome. We interpreted our findings in 38 light of bile acids being key molecular components regulating the gut microbiome composition 39 during amphibian development and metamorphosis. 40 41 Metatranscriptome 43 44 Background 45Metazoans are vehicles for microbial communities, also named microbiota. The microbiota 46 and its metazoan host have mutualistic interactions and are thought to adapt and evolve as 47 holobiont (Wilson and Sober, 1989;Gill et al., 2006; Zilber-Rosenberg and Rosenberg, 2008; 48 Bordenstein and Theis, 2015). Several studies have highlighted the importance of the 49 microbiota in the function and development of several organs such as the alimentary canal, the 50 nervous system and the tegument ...) (Sekirov et al., 2010;Sommer and Bäckhed, 2013; 51 Douglas, 2018). The dynamic interaction between a microbiota and its host is under intense 52 scrutiny especially for mammalian species and a handful of model organisms (Colston and 53 Jackson, 2016;Douglas, 2019). However, very little is currently known on the biotic and abiotic 54 interactions between the microbiota of even well-known and classic vertebrate model 55 organisms such as amphibians (Colston and Jackson, 2016;Douglas, 2019; Rebollar and Harris, 56 2019). 57The current paucity of knowledge on amphibian's microbiome is an historical thumb of 58 nose. Indeed in 1901, Olga Metchnikoff publ...

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Changes in intestinal microbiota of Bufo gargarizans and its association with body weight during metamorphosis

Cited by 39 publications

References 48 publications

The Changes in the Frog Gut Microbiome and Its Putative Oxygen-Related Phenotypes Accompanying the Development of Gastrointestinal Complexity and Dietary Shift

The Changes in the Frog Gut Microbiome and Its Putative Oxygen-Related Phenotypes Accompanying the Development of Gastrointestinal Complexity and Dietary Shift

Gut dysbacteriosis and intestinal disease: mechanism and treatment

Gut microbial ecology of Xenopus tadpoles across life stages

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