Changes in integrin expression during adipocyte differentiation

Liu, Jun; DeYoung, Stephanie M.; Zhang, Ming; Zhang, Mei; Cheng, Alan; Saltiel, Alan R.

doi:10.1016/j.cmet.2005.08.006

Cited by 168 publications

(174 citation statements)

References 68 publications

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“…4). We focused on integrin-␤1 because its molecular weight was consistent with the band, and previous studies report that integrins are involved in adipocyte differentiation (33)(34)(35). We found that ␣2,6-sialylation of integrin-␤1 in mouse VATs was significantly reduced in HFD mice compared with CON mice (Fig.…”

Section: St6gal1mentioning

confidence: 84%

The Inhibitory Role of α2,6-Sialylation in Adipogenesis

Kaburagi

Kizuka

Kitazume

et al. 2017

Journal of Biological Chemistry

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Edited by Gerald W. HartAdipose tissue plays critical roles in obesity and related diseases such as diabetes and cardiovascular diseases. Previous reports suggest that glycans, the most common posttranslational modifications, are involved in obesity-related diseases, but what type of glycan regulates adipogenesis during obesity remains unclear. In this study, we first quantified the mRNA levels of 167 genes (encoding 144 glycosyltransferases and 23 related enzymes) in visceral adipose tissues (VATs) from control mice and high-fat diet (HFD)-induced obese mice. We found that a gene encoding ␤-galactoside ␣2,6-sialyltransferase-1 (St6gal1), a key enzyme responsible for the biosynthesis of ␣2,6-linked sialic acid in N-linked glycans, was most down-regulated in VATs from obese mice. We confirmed the reduction in ␣2,6-sialic acid in VATs from obese mice and differentiated adipocyte model 3T3-L1 cells. Using proteomic analysis, integrin-␤1 was identified as one of the target ␣2,6-sialylated proteins in adipose tissues, and phosphorylation of its downstream molecule focal adhesion kinase was found to be decreased after HFD feeding. St6gal1 overexpression in differentiating 3T3-L1 cells inhibited adipogenesis with increased phosphorylation of focal adhesion kinase. Furthermore, St6gal1 knockout mice exhibited increased bodyweight and VAT weight after HFD feeding. The down-regulation of St6gal1 during adipogenesis was canceled by treatment with a DNA methyltransferase inhibitor, suggesting an involvement of epigenetic DNA methylation in St6gal1 silencing. Our findings suggest that ST6GAL1 has an inhibitory role in adipogenesis through integrin-␤1 activation, providing new insights into the roles and regulation mechanisms of glycans in adipocytes during obesity.

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Section: St6gal1mentioning

confidence: 84%

The Inhibitory Role of α2,6-Sialylation in Adipogenesis

Kaburagi

Kizuka

Kitazume

et al. 2017

Journal of Biological Chemistry

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“…We used integrin ␣6 as a marker for plasma membrane localization since it had been shown to be highly expressed on the cell surface of white adipocytes (31). Three-dimensional reconstructions of confocal microscopy optical sections showed that FATP1 is expressed throughout BAT and is clearly upregulated following a 12-h cold exposure ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Fatty Acid Transport Protein 1 Is Required for Nonshivering Thermogenesis in Brown Adipose Tissue

et al. 2006

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Nonshivering thermogenesis in brown adipose tissue (BAT) generates heat through the uncoupling of mitochondrial ␤-oxidation from ATP production. The principal energy source for this process is fatty acids that are either synthesized de novo in BAT or are imported from circulation. How uptake of fatty acids is mediated and regulated has remained unclear. Here, we show that fatty acid transport protein (FATP)1 is expressed on the plasma membrane of BAT and is upregulated in response to cold stimuli, concomitant with an increase in the rate of fatty acid uptake. In FATP1-null animals, basal fatty acid uptake is reduced and remains unchanged following cold exposure. As a consequence, FATP1 knockout (KO) animals display smaller lipid droplets in BAT and fail to defend their core body temperature at 4°C, despite elevated serum free fatty acid levels. Similarly, FATP1 is expressed by the BATderived cell line HIB-1B upon differentiation, and both fatty acid uptake and FATP1 protein levels are rapidly elevated following isoproterenol stimulation. Stimulation of fatty uptake by isoproterenol required both protein kinase A and mitogen-activated kinase signaling and is completely dependent on FATP1 expression, as small-hairpin RNA-mediated knock down of FATP1 abrogated the effect. Diabetes 55:3229 -3237, 2006

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“…Several preadipocyte cell lines undergo differentiation on treatment with a standard adipogenic cocktail consisting of IBMX, dexamethasone and insulin 35 . Both IBMX and dexamethasone trigger the activation of protein kinase A by increasing the intracellular concentration of cAMP, thereby promoting adipocyte differentiation 36 .…”

Section: Discussionmentioning

confidence: 99%

Regulation of MKL1 via actin cytoskeleton dynamics drives adipocyte differentiation

et al. 2014

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Cellular differentiation is regulated through activation and repression of defined transcription factors. A hallmark of differentiation is a pronounced change in cell shape, which is determined by dynamics of the actin cytoskeleton. Here we show that regulation of the transcriptional coactivator MKL1 (megakaryoblastic leukemia 1) by actin cytoskeleton dynamics drives adipocyte differentiation mediated by peroxisome proliferator-activated receptor g (PPARg), a master transcriptional regulator of adipogenesis. Induction of adipocyte differentiation results in disruption of actin stress fibres through downregulation of RhoA-ROCK signalling. The consequent rapid increase in monomeric G-actin leads to the interaction of G-actin with MKL1, which prevents nuclear translocation of MKL1 and allows expression of PPARg followed by adipogenic differentiation. Moreover, we found that MKL1 and PPARg act in a mutually antagonistic manner in the adipocytic differentiation programme. Our findings thus provide new mechanistic insight into the relation between the dynamics of cell shape and transcriptional regulation during cellular differentiation.

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Changes in integrin expression during adipocyte differentiation

Cited by 168 publications

References 68 publications

The Inhibitory Role of α2,6-Sialylation in Adipogenesis

The Inhibitory Role of α2,6-Sialylation in Adipogenesis

Fatty Acid Transport Protein 1 Is Required for Nonshivering Thermogenesis in Brown Adipose Tissue

Regulation of MKL1 via actin cytoskeleton dynamics drives adipocyte differentiation

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