Changes in Human Serum Dopamine-β-Hydroxylase Activity with Age

Freedman, Lewis S.; Ohuchi, Takeshi; Goldstein, Menek; Axelrod, Felicia B.; Fish, Irving; Dancis, Joseph

doi:10.1038/236310a0

Cited by 100 publications

(21 citation statements)

References 7 publications

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“…Serum dopaminebeta-hydroxylase was measured spectrophotometrically as previously described (20,21). Serum dopamine-beta-hydroxylase values for pediatric normal controls (n = 19, from 2 mo to 1 1-yr old) were 3±1 IU/liter, results consistent with previous reports (22,23). Serum values of > 5 IU/liter were considered abnormal.…”

Section: Human Subject Samplessupporting

confidence: 74%

Chromogranin A in children with neuroblastoma. Serum concentration parallels disease stage and predicts survival.

Hsiao

Seeger²,

Yu³

et al. 1990

J. Clin. Invest.

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Chromogranin A is an acidic protein costored and coreleased with catecholamines from storage vesicles. Its serum concentration is elevated in patients with peptide-producing endocrine neoplasia. We measured serum chromogranin A at the time of diagnosis in 34 children with all stages of neuroblastoma. With a sensitivity of 91% and specificity of 100%, serum chromogranin A emerged as a useful diagnostic tool for neuroblastoma, comparable to or better than other measurements such as neuron-specific enolase, ferritin, or dopamine-beta-hydroxylase. Mean serum chromogranin A correlated with disease stage (r = 0.76, P < 0.01). The relationship of prognosis (progression-free survival) to baseline serum chromogranin A, age, and disease stage was determined in 34 patients at risk for relapse, with a median followup period of 18 mo (range, 1-48 mo). The survival rate for patients with lower serum chromogranin A levels (< 190 ng/ml at the time of diagnosis) was 69%, whereas it was 30% for those with higher chromogranin A levels (P < 0.05). Furthermore, when subjects were additionally stratified by either age or stage, chromogranin A was an effective prognostic tool in patients who either were older than 1 yr (P < 0.005) or had more advanced disease (stage III or IV; P < 0.05). We conclude that serum chromogranin A in neuroblastoma is (a) a valuable (sensitive and specific) diagnostic tool, (b) a correlate of tumor burden, and (c) a useful predictor of survival. (J. Clin. Invest. 1990Invest. . 85:1555Invest. -1559 chromogranin -neuroblastoma

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Section: Human Subject Samplessupporting

confidence: 74%

Chromogranin A in children with neuroblastoma. Serum concentration parallels disease stage and predicts survival.

Hsiao

Seeger²,

Yu³

et al. 1990

J. Clin. Invest.

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“…Although serum D/3H activity has been reported to increase with age, no subjects over the age of 60 were examined in this study. 78 Using the less reliable spectrophotometric determination of D/3H, Annat et al 79 found no difference in D/3H levels between young and aged normotensive subjects.…”

Section: Effect Of Aging On Dopaminergic Mechanismsmentioning

confidence: 99%

Peripheral dopamine in pathophysiology of hypertension. Interaction with aging and lifestyle.

Küchel

Kuchel

1991

Hypertension

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Dopamine, an ancestral catecholamine, is physiologically natriuretic and vasodilating, thus essentially protecting against hypertension. Its actions are overshadowed by the opposite effects of its main biological partner, norepinephrine, and this is accentuated with aging. Clinical observations combined with molecular biology approaches to catecholamine-synthesizing and catecholamine-metabolizing enzymes and receptors permit the identification of some inborn defects. Subtle changes in the dopamine-norepinephrine balance may account for the enhanced peripheral noradrenergic activity seen in the setting of decreased dopaminergic activity in advanced age. These changes may contribute to the diminished ability of the aged kidney to excrete a salt load, as well as to the finding that systolic blood pressure increases with age in populations with a high, but not in those with a low, intake of salt. The attainment of advanced age in Western societies with adverse lifestyle changes (mental rather than physical stress, excess salt intake, overeating, sedentarism) appears to facilitate the development of hyperten-sion. The adaptation to all the preceding lifestyle changes necessitates an increased dopamine generation, which may initially compensate to maintain appropriate natriuresis and vasodila-tion since many patients with initial borderline essential hypertension express their sympathetic hyperfunction, in addition to increased norepinephrine release, by excessive dopamine release. However, the progression of hypertension is accompanied by a peripheral dopaminer-gic deficiency and diminished ability to excrete salt. This may represent an eventual inadequacy of a phylogenetically redundant system resulting in decreased natriuresis and vasodilation and may account for the responsiveness of established chronic hypertension to salt restriction, diuretics, and dopaminomimetic medication. (Hypertension 1991;18:709-721) O ver the last decade, it has become apparent that dopamine (DA) is an important neuro-transmitter in the peripheral nervous system. Nonneuronal (kidney, intestine, blood vessels) and neuronal (neural terminals, adrenal medulla) tissues are target organs for circulating DA and also for DA synthesized and secreted locally as a paracrine substance. DA receptors located extracellularly in plasma-exposed cellular membranes transduce their signals to the intracellular milieu through a variety of second messengers, including adenylate cyclase, phos-pholipase C, protein kinase C, and other protein kinases. Peripheral DA! receptors (typical for vascular smooth muscles and renal tubules) act via adenylate cyclase and phospholipase C stimulation, whereas DA 2 receptors presynaptically suppress the release of norepinephrine (NE) via adenylate cyclase inhibition. 1 DA has been shown to affect the actions of ion transporters as well as a variety of cellular enzymes and appears to be physiologically important in the control of renal function. The sum of the effects of physiological DA concentrations on the kidney (DA r med...

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“…The evaluation of the usefulness of serum levels of dopamine-,3-hydroxylase [3,4-dihydroxyphenylethylamine, ascorbate: oxygen oxidoreductase (/3-hydroxylating), EC 1.14.17.1, DO3H] as an index of sympathetic nervous system activity in man has been hindered by the extremely wide range of values seen in "normal" human serum samples (1)(2)(3)(4). This wide range of values appears to be genetically controlled (5,6) and may mask any variations due to differences in sympathetic activity.…”

mentioning

confidence: 99%

Measurement of human dopamine-beta-hydroxylase in serum by homologous radioimmunoassay.

Rush¹,

Thomas²,

Udenfriend³

1975

Proc. Natl. Acad. Sci. U.S.A.

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Human serum dopamine-I3-hydroxylase[3,4-dihydroxyphenylethylamine, ascorbate:oxygen oxidoreductase (B8-hydroxylating), EC 1.14.17.11 levels have been determined in 52 samples with a newly devised radioimmunoassay that utilizes purified human enzyme and its specific antiserum. The values obtained were in excellent agreement (correlation coefficient r = 0.98) with those obtained by measuring enzymatic activity in the same sera. Either assay technique, enzymatic or homologous radioimmunoassay, can be used for measurement of the enzyme in normal human serum.The evaluation of the usefulness of serum levels of dopamine-,3-hydroxylase [3,4-dihydroxyphenylethylamine, ascorbate: oxygen oxidoreductase (/3-hydroxylating), EC 1.14.17.1, DO3H] as an index of sympathetic nervous system activity in man has been hindered by the extremely wide range of values seen in "normal" human serum samples (1-4). This wide range of values appears to be genetically controlled (5, 6) and may mask any variations due to differences in sympathetic activity. Recently, using a radioimmunoassay developed for bovine DjOH, we measured human serum and compared these values with those obtained by enzyme assay (7). No correlation was found. On the other hand, Ebstein et al. (8), using a radioimmunoassay developed for the enzyme isolated from a human pheochromocytoma, found significant correlation between the enzymatic and immunoassays of individual human sera in one study (8) but not in another one (9). In order to help clarify the relationship between the two types of assay we have estimated individual serum samples by both an enzymatic and a homologous radioimmunoassayt for human D03H. The immunoassay was developed using purified human DOH isolated from adrenal glands obtained at autopsy, and its specific antibody.Abbreviation: D,3H, dopamine-,8-hydroxylase.

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Changes in Human Serum Dopamine-β-Hydroxylase Activity with Age

Cited by 100 publications

References 7 publications

Chromogranin A in children with neuroblastoma. Serum concentration parallels disease stage and predicts survival.

Chromogranin A in children with neuroblastoma. Serum concentration parallels disease stage and predicts survival.

Peripheral dopamine in pathophysiology of hypertension. Interaction with aging and lifestyle.

Measurement of human dopamine-beta-hydroxylase in serum by homologous radioimmunoassay.

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