Changes in heterosubtypic antibody responses during the first year of the 2009 A(H1N1) influenza pandemic

Freidl, Gudrun; Ham, Henk-Jan van den; Boni, Maciej F.; Bruin, Erwin de

doi:10.1038/srep20385

Cited by 11 publications

(9 citation statements)

References 50 publications

(93 reference statements)

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“…The prevalence of antibodies was highest for the H9, and lowest for the H7 antigens. As described elsewhere, a significant proportion of healthy blood donors had antibodies to H9 influenza virus antigens(Boni et al., ; Freidl et al., ), but the prevalence was higher in poultry workers for the most recent antigens (H9‐2009 and H9‐2011). Significantly higher titres were also detected to the most recent H5 antigens (H5‐2010E and H5‐2010H) for poultry workers compared to healthy blood donors (Figure a and b).…”

Section: Resultsmentioning

confidence: 75%

“…A recent study using PA in 13 different countries shows that with increasing age the reactivity to H7 and H9 antigens increases. This increase was associated with heterosubtypic responses to seasonal influenza virus types (Freidl et al., ). Therefore, possible differences between poultry workers and blood donors from older age groups might not be detectable because of those heterosubtypic responses to H9 antigens measured by PA.…”

Section: Discussionmentioning

confidence: 99%

“…Validation of this PA for antibody ascertainment has been extensively published elsewhere (Koopmans et al, 2011;Boni et al, 2013;Freidl, van den Ham, de Bruin, Boni, & Koopmans, 2016).…”

Section: Protein Microarray (Pa)mentioning

confidence: 99%

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Serological evidence for exposure to avian influenza viruses within poultry workers in southern China

Bruin

Zhang

et al. 2017

Zoonoses and Public Health

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The risk of infection with avian influenza viruses for poultry workers is relatively unknown in China, and study results are often biased by the notification of only the severe human cases. Protein microarray was used to detect binding antibodies to 13 different haemagglutinin (HA1-part) antigens of avian influenza A(H5N1), A(H7N7), A(H7N9) and A(H9N2) viruses, in serum samples from poultry workers and healthy blood donors collected in the course of 3 years in Guangdong Province, China. Significantly higher antibody titre levels were detected in poultry workers when compared to blood donors for the most recent H5 and H9 strains tested. These differences were most pronounced in younger age groups for antigens from older strains, but were observed in all age groups for the recent H5 and H9 antigens. For the H7 strains tested, only poultry workers from two retail live poultry markets had significantly higher antibody titres compared to blood donors.

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Section: Resultsmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 99%

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Serological evidence for exposure to avian influenza viruses within poultry workers in southern China

Bruin

Zhang

et al. 2017

Zoonoses and Public Health

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“…Therefore, age-matched controls are necessary to determine baseline level of pre-existing antibodies in different birth year cohorts. 13,22 A complex relationship between antibodies against seasonal and novel avian influenza viruses was observed in humans 23 and experimentally infected chicken and ferrets. 8 not by HI in primary H1N1 or H3N2 influenza virus-infected children.…”

Section: Discussionmentioning

confidence: 99%

Novel multiplex assay platforms to detect influenza A hemagglutinin subtype‐specific antibody responses for high‐throughput and in‐field applications

Trost

Weber

et al. 2017

Influenza Resp Viruses

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BackgroundDetections of influenza A subtype‐specific antibody responses are often complicated by the presence of cross‐reactive antibodies. We developed two novel multiplex platforms for antibody detection. The multiplexed magnetic fluorescence microsphere immunoassay (MAGPIX) is a high‐throughput laboratory‐based assay. Chembio Dual Path Platform (DPP) is a portable and rapid test that could be used in the field.MethodsTwelve recombinant globular head domain hemagglutinin (GH HA1) antigens from A(H1N1)pdm09 (pH1N1), A(H2N2), A(H3N2), A(H5N1), A(H7N9), A(H9N2), A(H13N9), B/Victoria lineage, B/Yamagata lineage viruses, and protein A control were used. Human sera from U.S. residents either vaccinated (with H5N1 or pH1N1) or infected with pH1N1 influenza viruses and sera from live bird market workers in Bangladesh (BDPW) were evaluated. GH HA1 antigens and serum adsorption using full ectodomain recombinant hemagglutinins from A(pH1N1) and A(H3N2) were introduced into the platforms to reduce cross‐reactivity.ResultsSerum adsorption reduced cross‐reactivity to novel subtype HAs. Compared to traditional hemagglutination inhibition or microneutralization assays, when serum adsorption and the highest fold rise in signals were used to determine positivity, the correct subtype‐specific responses were identified in 86%‐100% of U.S. residents exposed to influenza antigens through vaccination or infection (N=49). For detection of H5N1‐specific antibodies in sera collected from BDPW, H5 sensitivity was 100% (six of six) for MAGPIX, 83% (five of six) for DPP, H5 specificity was 100% (15/15), and cross‐reactivity against other subtype was 0% (zero of six) for both platforms.ConclusionMAGPIX and DPP platforms can be utilized for high‐throughput and in‐field detection of novel influenza virus infections.

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“…Recently, researchers have started to develop protein microarrays for influenza virus serology. 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 Protein microarrays are a high-throughput assay that can measure the magnitude and breadth of an antibody response. Like the ELISA (and unlike HI and MN assays), protein microarrays can also detect non-neutralizing antibodies, which are able to protect against influenza virus through effector functions such as antibody-dependent cell-mediated cytotoxicity and antibody-mediated cellular phagocytosis.…”

Section: Introductionmentioning

confidence: 99%

Development of an influenza virus protein microarray to measure the humoral response to influenza virus infection in mallards

Meade

Latorre‐Margalef

Stallknecht

et al. 2017

Emerging Microbes & Infections

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Avian influenza viruses pose a serious zoonotic threat, in part because current seasonal influenza virus vaccines only offer strain-specific protection, instead of heterosubtypic or universal protection against influenza virus infection. Understanding the humoral response to vaccination and natural infection in the broadest context possible is important to developing defenses against influenza zoonosis. Protein microarrays are a novel platform well suited to assaying the humoral immune response broadly and efficiently. We developed an influenza virus protein microarray (IVPM) that could be used to assay sera from many species, including humans. Waterfowl such as mallard ducks are natural reservoirs for many influenza A viruses, but their humoral immune response to infection is poorly understood. To establish this technology, we assayed sera from mallards experimentally infected with two low-pathogenic common avian influenza viruses (H3N8 and H4N5) for reactivity to influenza virus hemagglutinin (HA) by IVPM. The IVPM results correlated well with results from an established enzyme-linked immunosorbent assay, supporting the validity of the IVPM as a serological assay in influenza virus research. Interestingly, successive infection with H3N8 followed by H4N5 virus in mallard ducks induced antibodies that were broadly reactive against group 2 hemagglutinins. We also analyzed sera from wild mallards and observed serological evidence for infection in those sera. With serological information, it may be possible to infer infection history of wild avian species and gain a better understanding of the infection dynamics of influenza viruses in their natural reservoir. This might ultimately lead to interventions that enhance our pandemic preparedness.

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Changes in heterosubtypic antibody responses during the first year of the 2009 A(H1N1) influenza pandemic

Cited by 11 publications

References 50 publications

Serological evidence for exposure to avian influenza viruses within poultry workers in southern China

Serological evidence for exposure to avian influenza viruses within poultry workers in southern China

Novel multiplex assay platforms to detect influenza A hemagglutinin subtype‐specific antibody responses for high‐throughput and in‐field applications

Development of an influenza virus protein microarray to measure the humoral response to influenza virus infection in mallards

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