Changes in HER2 status after neoadjuvant treatment of breast cancer.

Skálová, Helena; Dundr, Pavel; Povýšil, C; Pribylova, O.; Petruželka, Luboš; Tvrdík, Daniel

doi:10.1200/jco.2011.29.15_suppl.e11110

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Immunohistochemistry was performed using the avidin-biotin complex (ABC) method as was described in our previous work [17]. Primary antibodies were applied against c-erbB-2 protein (1:100, clone 5A2, Novocastra); ER (1:20, clone ER-6F11, Novocastra); PR (1:100, clone PGR-312, Novocastra); Ki-67 (1:50, clone MIB-1, DakoCytomation); CK5,6 (1:50, clone D5/16B4, DakoCytomation), CK8 (ready to use, clone 35bH11, DakoCytomation), CK18 (1:50, clone DC10, DakoCytomation) and cleaved caspase-3 (1:250, Asp175, Cell Signaling Technology, Beverly, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

Apoptosis – associated genes and their role in predicting responses to neoadjuvant breast cancer treatment

et al. 2012

View full text Add to dashboard Cite

SummaryBackgroundNeoadjuvant chemotherapy is used in the treatment of breast carcinoma because it substantially reduces the size of the primary tumor and lymph node metastases. The present study investigated biomarkers that can predict a pathologic response to the therapy.Material/MethodsThe role of apoptosis in regression of the tumors after neoadjuvant chemotherapy was determined by TUNEL and anti-active caspase 3 assay. The transcriptional profile of 84 key apoptosis genes was evaluated in both pre-therapeutically obtained tumor tissue by core needle biopsy and in specimens removed by final surgery, using a pathway-specific real-time PCR assay. Obtained data were analyzed by hierarchical cluster analysis and correlation analysis. The immunohistochemical profile of each tumor was determined using the standard ABC method.ResultsOn the basis of a hierarchical cluster analysis of 13 significantly changed genes, we divided patients into good and poor prognosis groups, which correlate well with progression-free survival. In the good prognosis group, we found a statistically significant down-regulation of the expression of MCL1 and IGF1R genes after neoadjuvant treatment. We also found a statistically significant overexpression of BCL2L10, BCL2AF1, CASP8, CASP10, CASP14, CIDEB, FADD, HRK, TNFRSF25, TNFSF8 and CD70 genes. In contrast, we found up-regulation of IGF1R after the treatment in the group with poor prognosis.ConclusionsGene expression profiling using real-time PCR assay is a valuable research tool for the investigation of molecular markers, which reflect tumor biology and treatment response.

show abstract

Section: Methodsmentioning

confidence: 99%