Changes in growth, biochemical, and chemical characteristics and alteration of the antioxidant defense system in the leaves of tea clones (Camellia sinensis L.) under drought stress

Chaeikar, Sanam Safaei; Marzvan, Saeideh; Khiavi, Shahin Jahangirzadeh; Rahimi, Mehdi

doi:10.1016/j.scienta.2020.109257

Cited by 20 publications

(14 citation statements)

References 26 publications

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“…Thus, the accumulation of proline plays a key role under drought stress as an antioxidant or through stabilizing macromolecules during water stress [79]. The enhancement of DPPH activity observed in the more stressed plants is in line with other studies [61,80]. The total antioxidant activity is the combined results of all enzymatic and non-enzymatic antioxidant activity in plants subjected to abiotic stresses.…”

Section: Discussionsupporting

confidence: 77%

Morphological, Physiological, and Biochemical Responses of Zinnia to Drought Stress

Toscano

Romano

2021

Horticulturae

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Bedding plants in the nursery phase are often subject to drought stress because of the small volume of the containers and the hydraulic conductivity of organic substrates used. To analyse the morphological, physiological, and enzymatic responses of zinnia (Zinnia elegans L.) plants at different irrigation levels, four treatments were performed: irrigated at 100% (100% field capacity, FC); light deficit irrigation (75% FC), medium deficit irrigation (50% FC), and severe deficit irrigation (25% FC). The growth of zinnia was significantly influenced by drought stress treatments. Different morphological parameters (dry biomass, leaf number, root to shoot ratio (R/S)) were modified only in the more severe drought stress treatment (25% FC). The stomata density increased in 50% FC and 25% FC, while the stomata size was reduced in 25% FC. The net photosynthesis, stomatal conductance, and transpiration were reduced in 50% FC and 25% FC. The relative water content (RWC) was reduced in 25% FC. Severe drought stress (25% FC) increased proline content up to seven-fold. Catalase (CAT), peroxidase (GPX), and superoxide dismutase (SOD) activity significantly increased in 50% FC and 25% FC. Principal component analysis (PCA) showed that the morphological and physiological parameters were mostly associated with the 100% FC and 75% FC treatments of the biplot, whereas the stomata density, R/S ratio, and antioxidant enzymes (GPX, CAT) were associated with 50% FC, and proline and DPPH were associated with 25% FC, respectively.

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Section: Discussionsupporting

confidence: 77%

Morphological, Physiological, and Biochemical Responses of Zinnia to Drought Stress

Toscano

Romano

2021

Horticulturae

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“…It protects plants from various abiotic and biotic stresses, such as the fungus Colletotrichum gloeosporioides [61]. Mild drought increases the level of caffeine, while severe drought decreases it in most tea cultivars under field conditions [1,60,62]. However, PEG treatment continues to reduce the contents of caffeine owing to the down-regulation of TCS [63].…”

Section: Changes In the Quality Components In Tea Shoots Under Drough...mentioning

confidence: 99%

Molecular and Metabolic Changes under Environmental Stresses: The Biosynthesis of Quality Components in Preharvest Tea Shoots

et al. 2022

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Severe environments impose various abiotic stresses on tea plants. Although much is known about the physiological and biochemical responses of tea (Camellia sinensis L.) shoots under environmental stresses, little is known about how these stresses impact the biosynthesis of quality components. This review summarizes and analyzes the changes in molecular and quality components in tea shoots subjected to major environmental stresses during the past 20 years, including light (shade, blue light, green light, and UV-B), drought, high/low temperature, CO2, and salinity. These studies reveal that carbon and nitrogen metabolism is critical to the downstream biosynthesis of quality components. Based on the molecular responses of tea plants to stresses, a series of artificial methods have been suggested to treat the pre-harvest tea plants that are exposed to inhospitable environments to improve the quality components in shoots. Furthermore, many pleiotropic genes that are up- or down-regulated under both single and concurrent stresses were analyzed as the most effective genes for regulating multi-resistance and quality components. These findings deepen our understanding of how environmental stresses affect the quality components of tea, providing novel insights into strategies for balancing plant resistance, growth, and quality components in field-based cultivation and for breeding plants using pleiotropic genes.

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“…A 0.5g of fresh leaf samples was powdered in liquid nitrogen and homologized in 5 ml of potassium phosphate buffer (100 mM, pH 7.0) including 0.5% Triton X-100, 2% (w/v) N-Vinylpyrrolidinone, 5 mM Methylene diaminete traacetic acid disodium salt dried and 1 mM ascorbic acid [5]. Homogenates were then centrifuged at 12,000 for 20 min at 4°C and the supernatants were used to verify the interest of catalase (CAT, EC1.11.1.6) according to Aebi (El Bedawy 2014).…”

Section: Activity Of Antioxidant Enzymesmentioning

confidence: 99%

“…The CsDCL1 gene primer sequences were 5-CGGTTTGAACACGCAGAA-3 and 3-GAGGCAGGAACAGGGTAG. CsActin 5-CAACCACAAGGGCTAACAGAG-3 and 3-GAATCCAGCACGATACCAGT were the housekeeping gene sequences (5). The Thermal Cycler Bio rad Real-Time System II (TaKaRa, Shiga, Japan) and the SYBR kit were applied for real-time PCR.…”

Section: Plant Defense Genes Expressionmentioning

confidence: 99%

“…Water de ciency stress is the main serious abiotic stress affecting plant growth and has become the preliminary cause of crop productivity and quality declines [2]. Most plants experienced growth rate, leaf water content (LWC), relative water content, chlorophyll content, photosynthetic frequency, stomatal conductance, and transpiration frequency changes due to water-de cit conditions, which controlled inadequate plant growth and yield production [3][4][5]. Antioxidant enzymes, i.e., catalase (CAT), peroxidase (POD), and glutathione reductase, showed lower activity under waterde cit stress, which causes oxidative tension in plant cells, but increases malondialdehyde concentration, i.e., hydrogen peroxide [6].…”

Section: Introductionmentioning

confidence: 99%

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Grafting enhances drought stress tolerance by regulating stress-responsive gene expression and antioxidant enzyme activities in cucumbers

Shehata

Omar

El_Sayed

et al. 2022

Preprint

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Water scarcity is a major limiting factor for crop yield production in arid and water-stressed areas worldwide. Cucumber plants have a high transpiration ratio and are vulnerable to water stress. Grafting commercial cultivars onto selected strong rootstocks has been useful in mitigating the effects of water stress. Therefore, this study aimed to evaluate the possibility of using a novel rootstock plant’s tolerance to water-deficit stress in inducing drought tolerance in cucumbers by activating the stress-response gene expression and the antioxidant system, which improved the cucumber quality and yield under water-deficit conditions. This field experiment was conducted for >2 years, 2020 and 2021, with five water stress cultivars (i.e., rootstock) and drought-sensitive cultivar Luerans (i.e., a scion). They were subjected to various deficit irrigation levels for 12 days, and their agro-physiological and molecular responses to water-deficit stress were assessed. Our results showed that the agronomical parameters, including the leaf area, leaf water content, number of leaves, plant height, root dry matter shoot dry matter, rates of leaf appearance and stem elongation, and total yield significantly increased with grafted cucumber plants than with nongrafted cucumber plants (control) under normal and stressful conditions. The physiological parameters revealed that higher catalase and peroxidase activities, abscisic acid levels, gibberellic acid accumulation, and lower lipid peroxidation (malondialdehyde) significantly increased the plant growth rate in rootstock-grafted genotypes. Furthermore, the real-time polymerase chain reaction expression results revealed that transcript levels of the stress-response genes CsAGO1 and CsDCLs increased rapidly and continuously in five rootstock grafting. Concurrently, these findings suggest that grafting with local varieties of novel drought-tolerant rootstock genotypes could improve water stress tolerance in drought-sensitive cucumber cultivars.

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Changes in growth, biochemical, and chemical characteristics and alteration of the antioxidant defense system in the leaves of tea clones (Camellia sinensis L.) under drought stress

Cited by 20 publications

References 26 publications

Morphological, Physiological, and Biochemical Responses of Zinnia to Drought Stress

Morphological, Physiological, and Biochemical Responses of Zinnia to Drought Stress

Molecular and Metabolic Changes under Environmental Stresses: The Biosynthesis of Quality Components in Preharvest Tea Shoots

Grafting enhances drought stress tolerance by regulating stress-responsive gene expression and antioxidant enzyme activities in cucumbers

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