Changes in gravitational forces induce the modification of Arabidopsis thaliana silique pedicel positioning

Wei, Ning; Tan, Chao; Qi, Bin; Zhang, Yue; Xu, Guoxin; Zheng, Huiqiong

doi:10.1093/jxb/erq200

Cited by 14 publications

(13 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…sgr1 , sgr2 , sgr3 (Fukaki et al ., ), zigzag ( zig )/ sgr4 , sgr5 , sgr6 (Yamauchi et al ., ), grv2 ( gravitropism defective 2 )/ sgr8 (Silady et al ., ), lazy1 (Li et al ., ; Yoshihara and Iino, ), tac1 ( tiller angle control 1 ) (Yu et al ., ) and now atlazy1 mutants. Even the angle between the inflorescence stem and fruit pedicel is affected by gravity (Wei et al ., ). The contribution of AtLAZY1 on the fruit‐bearing lateral branches of the Arabidopsis inflorescence is as large as any of these other examples.…”

Section: Discussionmentioning

confidence: 97%

AtLAZY1 is a signaling component required for gravitropism of the Arabidopsis thaliana inflorescence

2013

View full text Add to dashboard Cite

SUMMARYThe present study identified a family of six A. thaliana genes that share five limited regions of sequence similarity with LAZY1, a gene in Oryza sativa (rice) shown to participate in the early gravity signaling for shoot gravitropism. A T-DNA insertion into the Arabidopsis gene (At5g14090) most similar to LAZY1 increased the inflorescence branch angle to 81°from the wild type value of 42°. RNA interference lines and molecular rescue experiments confirmed the linkage between the branch-angle phenotype and the gene consequently named AtLAZY1. Time-resolved gravitropism measurements of atlazy1 hypocotyls and primary inflorescence stems showed a significantly reduced bending rate during the first hour of response. The subcellular localization of AtLAZY1 protein was investigated to determine if the nuclear localization predicted from the gene sequence was observable and important to its function in shoot gravity responses. AtLAZY1 fused to green fluorescent protein largely rescued the branch-angle phenotype of atlazy1, and was observed by confocal microscopy at the cell periphery and within the nucleus. Mutation of the nuclear localization signal prevented detectable levels of AtLAZY1 in the nucleus without affecting the ability of the gene to rescue the atlazy1 branch-angle phenotype. These results indicate that AtLAZY1 functions in gravity signaling during shoot gravitropism, being a functional ortholog of rice LAZY1. The nuclear pool of the protein appears to be unnecessary for this function, which instead relies on a pool that appears to reside at the cell periphery.

show abstract

Section: Discussionmentioning

confidence: 97%

AtLAZY1 is a signaling component required for gravitropism of the Arabidopsis thaliana inflorescence

2013

View full text Add to dashboard Cite

show abstract

“…Histochemical GUS staining was performed as described by Wei et al . (). Distance of the region of the root exhibiting auxin‐induced GUS expression was measured with Image J.…”

Section: Methodsmentioning

confidence: 97%

Modulation of root‐skewing responses by KNAT1 in Arabidopsis thaliana

Zheng

2013

The Plant Journal

Self Cite

View full text Add to dashboard Cite

SUMMARYThe KNOTTED1 homeobox (KNOX) family transcription factors are essential for stem cell establishment and maintenance and regulate various aspects of development in all green plants. Expression patterns of the KNOX genes in the roots of plants have been reported, but their role in development remains unclear. Here we show how the KNAT1 gene is specifically involved in root skewing in Arabidopsis. The roots of two mutant alleles of KNAT1 (bp-1 and bp-5) exhibited exaggerated skewing to the right of gravity when grown on both vertical and tilted agar medium surfaces. This skewing phenotype was enhanced by treatments with low concentrations of propyzamide, and required auxin transport. The KNAT1 mutation substantially decreased basipetal auxin transport and increased auxin accumulation in the roots. Using a PIN2-GFP reporter and western blot analysis, we found that this alteration in auxin transport was accompanied by a decrease in PIN2 levels in the root tip. Decreased PIN2 expression in the mutant roots was not accompanied by reduced mRNA levels, suggesting that the KNAT1 mutations affected PIN2 expression at the posttranscriptional level. In vivo visualization of intracellular vacuolar targeting indicated that vacuolar degradation of PIN2-GFP was significantly promoted in the root tips of the bp allelic mutants. Together, these results demonstrate that KNAT1 negatively modulates root skewing, possibly by regulating auxin transport.

show abstract

“…5H), which probably did not come to the notice of previous researchers. In mature plants, the lateral organs generally do not remain parallel to the gravity vector but tend to exist at a particular angle with respect to the vertical growth axis (Wei et al, 2010). The angle between the pedicel and the inflorescence stem influences both the architecture and the yield potential of the plants (Wang and Li, 2008).…”

Section: Osbzip48 Is a Functional Ortholog Of Athy5mentioning

confidence: 99%

OsbZIP48, a HY5 Transcription Factor Ortholog, Exerts Pleiotropic Effects in Light-Regulated Development

2017

View full text Add to dashboard Cite

Plants have evolved an intricate network of sensory photoreceptors and signaling components to regulate their development. Among the light signaling components identified to date, HY5, a basic leucine zipper (bZIP) transcription factor, has been investigated extensively. However, most of the work on HY5 has been carried out in Arabidopsis (Arabidopsis thaliana), a dicot. In this study, based on homology search and phylogenetic analysis, we identified three homologs of AtHY5 in monocots; however, AtHYH (HY5 homolog) homologs are absent in the monocots analyzed. Out of the three homologs identified in rice (Oryza sativa), we have functionally characterized OsbZIP48. OsbZIP48 was able to complement the Athy5 mutant. OsbZIP48 protein levels are developmentally regulated in rice. Moreover, the OsbZIP48 protein does not degrade in dark-grown rice and Athy5 seedlings complemented with OsbZIP48, which is in striking contrast to AtHY5. In comparison with AtHY5, which does not cause any change in hypocotyl length when overexpressed in Arabidopsis, the overexpression of full-length OsbZIP48 in rice transgenics reduced the plant height considerably. Microarray analysis revealed that OsKO2, which encodes ent-kaurene oxidase 2 of the gibberellin biosynthesis pathway, is down-regulated in OsbZIP48OE and up-regulated in OsbZIP48 KD transgenics as compared with the wild type. Electrophoretic mobility shift assay showed that OsbZIP48 binds directly to the OsKO2 promoter. The RNA interference lines and the T-DNA insertional mutant of OsbZIP48 showed seedling-lethal phenotypes despite the fact that roots were more proliferative during early stages of development in the T-DNA insertional mutant. These data provide credible evidence that OsbZIP48 performs more diverse functions in a monocot system like rice in comparison with its Arabidopsis ortholog, HY5.

show abstract

Changes in gravitational forces induce the modification of Arabidopsis thaliana silique pedicel positioning

Cited by 14 publications

References 49 publications

AtLAZY1 is a signaling component required for gravitropism of the Arabidopsis thaliana inflorescence

AtLAZY1 is a signaling component required for gravitropism of the Arabidopsis thaliana inflorescence

Modulation of root‐skewing responses by KNAT1 in Arabidopsis thaliana

OsbZIP48, a HY5 Transcription Factor Ortholog, Exerts Pleiotropic Effects in Light-Regulated Development

Contact Info

Product

Resources

About