Changes in granulopoiesis detected by in vitro colony formation in acute lymphatic leukaemia

Morris, T. C. M.; Butler, Melissa G.; Muldrew, J G; McNeill, T. A.; Bridges, J. M.

doi:10.1038/bjc.1977.130

Cited by 7 publications

(4 citation statements)

References 7 publications

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“…The results from agar growth of marrow cells in ALL showed either sparse growth of cell aggregates or no growth at all. However, there was no difference in remission frequency or survival time between these 2 groups consistent with results of previous studies (6,8,12,13). The karyotypic findings in ALL agree with results in previous reports, but the number of patients is too limited in this study for detection of prognostic implications of specific aberrations in ALL which have been documented previously (16,17).…”

Section: Discussionsupporting

confidence: 92%

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The relationship between growth in agar, karyotype and prognosis in acute leukaemia

Gustavsson

Mitelman

Olofsson

et al. 1984

Scandinavian Journal of Haematology

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The growth pattern in agar culture and the karyotype of bone marrow cells were studied in 79 patients with untreated acute non‐lymphocytic leukaemia (ANLL). Results were divided into the following groups: (A) colony and cluster formation; (B) growth of less than 600 small clusters per 105 cells; (C) growth of more than 600 small clusters; (D) no growth in agar. Cytogenetically, the patients were divided into 3 categories: NN, normal metaphases only; AN, both abnormal and normal metaphases and AA, abnormal metaphases only. An association was seen between growth pattern and karyotype: the majority of NN patients (33/37) belonged to group (A+B) while in group (C+D) 20/24 patients were AN or AA. 37 patients were prognostically evaluable. The growth pattern in agar but not the cytogenetic pattern had prognostic implications. 25 patients with acute lymphocytic leukaemia (ALL) were also studied at diagnosis. Different growth patterns in agar had no impact on prognosis. No relationship was detected between growth pattern and karyotype in ALL.

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Section: Discussionsupporting

confidence: 92%

“…For instance, growth of colonies and clusters is usually combined with a favourable prognosis (3,11) whereas no growth or an excessive production of only small clusters is associated with poor prognosis (3, 8,10,11). But prognostic implications of growth pattern in vitro of acute lymphocytic leukaemia (ALL) have not been found (8,(11)(12)(13).…”

mentioning

confidence: 99%

The relationship between growth in agar, karyotype and prognosis in acute leukaemia

Gustavsson

Mitelman

Olofsson

et al. 1984

Scandinavian Journal of Haematology

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“…There was generally a lack of correlation between the results of CFU-GM in BM and in PB [24]. Moreover, numbers of colonies and clusters were not found to be correlated with the clinical features or outcome in ALL [11, 25]. …”

Section: Discussionmentioning

confidence: 99%

Granulocyte-Macrophage Colony-Forming-Unit Assay and Liquid Suspension Culture of Bone Marrow and Peripheral Blood in Newly Diagnosed Childhood Acute Lymphoblastic Leukemia

et al. 1998

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Granulocyte-macrophage colony-forming-unit (CFU-GM)-assays and/or liquid suspension cultures of bone marrow (BM) and peripheral blood (PB), using human placental conditioned medium as a source of colony-stimulating activity, were performed in 125 children with newly diagnosed acute lymphoblastic leukemia (ALL). Unlike the previous small series which invariably showed poor BM granulocyte-macrophage colony and cluster formations, we found 3 growth patterns. A poor-growth pattern was seen in 101 patients. A low-growth pattern with BM colony and cluster formations in the lower range of the normal limits was observed in 6 patients. All 6 patients had low-risk ALL, and the in vitro growth may reflect the residual normal CFU-GM in BM. Thirdly an over-growth pattern was noted in one patient with refractory ALL. In 49 cases where liquid suspension cultures of BM were performed, 4 had a normal-growth pattern and 42 had apoptotic cells and/or few residual granulocytic/monocytic cells after 7 days of culture. However, in both B cell ALL cases and in 1 refractory T cell ALL case with hyperleukocytosis, repetitive liquid suspension cultures of BM and PB consistently revealed an extensive growth of lymphoblasts. Thus, an over-growth pattern in either the BM CFU-GM assay or liquid suspension culture of BM or PB in children with ALL at diagnosis may indicate a refractory or aggressive disease. A low-growth pattern in the BM CFU-GM assay in children with ALL at diagnosis probably predicts a favorable outcome.

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“…Although invitro clonal assays for the granulocytic-monocytic progenitor cells CFU-GM and for the erythroid progenitors BFU-E and CFU-E have been used to study derangements of haemopoiesis in these patients (Brown & Carbone. 1971: Duttera et al, 1973Morris et al, 1977;Mangalik et al, 1977;Haworth et al 1982;Eridani et al, 1983;Peschel et al, 1983: Urabe et al, 1979, no information is yet available on the changes in the pluripotent progenitor cell pool. However, the description of an in vitro clonal assay for pluripotent colony-forming cells (CFU-GEMM) which upon culture give rise to colonies containing granulocytes, erythrocytes, macrophages and megakaryocytes (Fauser & Messner,19 78) and are thus supposed to be a close progeny of the true repopulating stem cell (Ogawa et al 1983) now allows the analysis of the quantitative and qualitative changes of the pluripotent progenitor cell pool in these patients.…”

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Effect of intensified chemotherapy on the pluripotent haematopoietic progenitor cells CFU‐GEMM in adult acute lymphoblastic leukaemia

Ganser

Hoelzer

1986

Br J Haematol

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In order to analyse the short-term and long-term effects of intensified induction chemotherapy, the frequency, cellular composition and proliferative state of the pluripotent haemopoietic progenitor cells CFU-GEMM was investigated in a total of 35 patients with acute lymphoblastic or acute undifferentiated leukaemia at diagnosis, as well as during and after therapy. At diagnosis, the number of CFU-GEMM/ml bone marrow aspirate was significantly reduced to 12.1 (95% confidence interval 2.1-70) as compared to healthy controls (50.4/ml; 95% confidence interval 66-3846/ml). While immediately after the end of induction therapy, the respective values for CFU-GEMM were still decreased (30.4/ml; 95% confidence interval 8.4-108); values not significantly different from normal were reached within the following 4 weeks. During and after cessation of maintenance therapy again no significant deviation from normal values was observed. Immunological analysis of single colonies revealed that during the early phase after remission induction only 60 +/- 7% of mixed colonies contained megakaryocytic cells (normal 86 +/- 3%; P less than 0.01) while normal values were found during and after maintenance therapy. Cell cycle analysis disclosed highly increased proliferative activity of pluripotent progenitor cells during marrow regeneration after induction therapy as well as during maintenance therapy, but a return to the resting state after cessation of maintenance therapy. It is concluded that this intensive chemotherapy regimen does not result in any apparent long-term damage to the pluripotent haemopoietic progenitor cells.

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Changes in granulopoiesis detected by in vitro colony formation in acute lymphatic leukaemia

Cited by 7 publications

References 7 publications

The relationship between growth in agar, karyotype and prognosis in acute leukaemia

The relationship between growth in agar, karyotype and prognosis in acute leukaemia

Granulocyte-Macrophage Colony-Forming-Unit Assay and Liquid Suspension Culture of Bone Marrow and Peripheral Blood in Newly Diagnosed Childhood Acute Lymphoblastic Leukemia

Effect of intensified chemotherapy on the pluripotent haematopoietic progenitor cells CFU‐GEMM in adult acute lymphoblastic leukaemia

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