“…The one-dimensional NMR spectrum of the simpler compound, G2, clearly displays two characteristic ␣-NeuAc H-3eq resonances at 2.769 and 2.727 ppm, as well as four -anomeric resonances ( 3 J 1,2 ϭ 7-9 Hz) in the downfield region, of which that at 4.512 ppm could be tentatively assigned as the -GlcNAc H-1, that at 4.160 ppm most likely as the -Glc H-1, and those at 4.248 and 4.117 ppm as two -Gal H-1, in agreement with the glycosyl composition analysis. Based on analogy to published NMR data, the rather upfield chemical shift of one of the -Gal H-1 resonances suggested the possibility of a type 1 chain core, as only the terminal -Gal H-1 of Lc 4 Cer has ever been observed upfield of 4.15 ppm (18). Three NAc methyl singlets (1.888, 1.875, and 1.759 ppm) are likewise consistent with the presence of two ␣-NeuAc and one -GlcNAc residue in the ganglioside; moreover, the chemical shifts of the two more downfield singlets suggested the presence of NeuAc in both ␣233 and ␣236 linkages, respectively (10, 17, 20).…”