Changes in glucosinolates content in Brassica oleracea modulate disease severity caused by Xanthomonas campestris pv. campestris

Madloo, Pari; Márquez, Margarita Lema; Francisco, Marta; Soengas, Pilar

doi:10.17660/actahortic.2018.1202.11

Cited by 2 publications

(5 citation statements)

References 8 publications

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“…Aliphatic GSL (glucoiberverin, indolic; glucobrassicin and mythoxyglucobrassicin) was found to increase in cabbage lines resistant to Mycosphaerella brassicicola [ 34 ]. The aliphatic GSL compound, sinigrin showed a potential role in inhibiting the growth of S. sclerotiorum in kale and Arabidopsis [ 23 , 46 ]. It was reported that the aliphatic gluconapin can defend B. rapa plants against Xcc pathogen [ 17 ], thereby a higher amount of gluconapin and hydroxyglucobrassicin was found in resistant B. rapa cultivar [ 43 ].…”

Section: Discussionmentioning

confidence: 99%

“…In kale, higher hydroxyglucobrassicin exhibited resistant to the Xcc pathogen [ 46 ]. A study on kale and Xcc pathogen interactions suggested that the indolic GSLs compound (glucobrassicin) showed as more effective than aliphatic GSLs in disease resistance [ 23 ]. Therefore, it could be concluded that increased level of aliphatic and indolic compounds may confer resistance against Xcc pathogen in cabbage.…”

Section: Discussionmentioning

confidence: 99%

“…Many reports have concluded that resistance to necrotrophs, biotrophs and hemibiotrophs are related to indolic GSLs content in Brassica crops [ 22 ]. In kale, two types of aliphatic GSLs (sinigrin and glucoiberin) and one indolic GSL (glucobrassicin) in leaves are modulated upon Xcc infection [ 23 ]. In Arabidopsis , GSL is hydrolyzed by a myrosinase gene, PENETRATION2 ( PEN2 ), and accumulated as indolic 4-methoxy-glucobrassicin in cells upon expression of CYP81F2 gene in response against fungal pathogen infection [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

“…GSL-hydrolyzed products (GHP) were found as effective to control several plant pathogenic bacteria, such as Erwinia chrysanthemi , Agrobacterium tumefaciens , Pseudomonas tomato , Pseudomonas cichorii , X. juglandis , X. campestris [ 11 ]. Recently, a study on the interaction of kale and Xcc pathogen suggested that indolic GSL compound (glucobrassicin) is more effective in decreasing disease severity than aliphatic GSL compounds [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

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Glucosinolate Profile and Glucosinolate Biosynthesis and Breakdown Gene Expression Manifested by Black Rot Disease Infection in Cabbage

Rubel

Abuyusuf

Nath

et al. 2020

Plants

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Cabbage (Brassica oleracea var. capitata) is an economically important crop in the family Brassicaceae. Black rot disease is a top ranked cabbage disease, which is caused by Xanthomonas campestris pv. campestris (Xcc) and may reduce 50% crop loss. Therefore, we need a clear understanding of black rot disease resistance for sustainable disease management. The secondary metabolites, like Glucosinolate (GSL) presents in Brassica species, which plays a potential role in the defense mechanism against pathogens. However, there is little known about GSL-regulated resistance mechanisms and GSL biosynthesis and the breakdown related gene expression after black rot disease infection in cabbage. In this study, relative expression of 43 biosynthetic and breakdown related GSLs were estimated in the black rot resistant and susceptible cabbage lines after Xcc inoculation. Ten different types of GSL from both aliphatic and indolic groups were identified in the contrasting cabbage lines by HPLC analysis, which included six aliphatic and four indolic compounds. In the resistant line, nine genes (MYB122-Bol026204, MYB34-Bol017062, AOP2-Bo9g006240, ST5c-Bol030757, CYP81F1-Bol017376, CYP81F2-Bol012237, CYP81F4-Bol032712, CYP81F4-Bol032714 and PEN2-Bol030092) showed consistent expression patterns. Pearson’s correlation coefficient showed positive and significant association between aliphatic GSL compounds and expression values of ST5c-Bol030757 and AOP2-Bo9g006240 genes as well as between indolic GSL compounds and the expression of MYB34-Bol017062, MYB122-Bol026204, CYP81F2-Bol012237, CYP81F4-Bol032712 and CYP81F4-Bol032714 genes. This study helps in understanding the role of GSL biosynthesis and breakdown related genes for resistance against black rot pathogen in cabbage, which could be further confirmed through functional characterization either by overexpression or knock-out mutation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Glucosinolate Profile and Glucosinolate Biosynthesis and Breakdown Gene Expression Manifested by Black Rot Disease Infection in Cabbage

Rubel

Abuyusuf

Nath

et al. 2020

Plants

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“…Previous studies suggested that the breakdown product of 4-methoxyindole-3-ylmethyl glucosinolate (4MI3G) performed effective functions in the process of plants defending themselves from fungi and pathogens [ 23 ]. Meanwhile, glucobrassicin, a compound of IGSs, conferred stronger disease resistance than AGS compounds in kale ( Brassica oleracea ) [ 24 ]. Furthermore, hydrolysates of IGSs are involved in plant resistance to B. cinerea [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

BcWRKY33A Enhances Resistance to Botrytis cinerea via Activating BcMYB51-3 in Non-Heading Chinese Cabbage

Wang

Zheng

Xiao

et al. 2022

IJMS

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The transcription factor WRKY33 is a vital regulator of the biological process of the necrotrophic fungus Botrytis cinerea (B. cinerea). However, its specific regulatory mechanism remains to be further investigated. In non-heading Chinese cabbage (NHCC, Brassica campestris (syn. Brassica rapa) ssp. Chinensis), our previous study showed that BcWRKY33A is induced not only by salt stress, but also by B. cinerea infection. Here, we noticed that BcWRKY33A is expressed in trichomes and confer plant defense resistance. Disease symptoms and qRT-PCR analyses revealed that BcWRKY33A-overexpressing and -silencing lines were less and more severely impaired, respectively, than wild type upon B. cinerea treatment. Meanwhile, the transcripts’ abundance of indolic glucosinolates’ (IGSs) biosynthetic genes is consistent with plants’ B. cinerea tolerance. Identification and expression pattern analysis of BcMYB51s showed that BcMYB51-3 has a similar trend to BcWRKY33A upon B. cinerea infection. Moreover, BcWRKY33A directly binds to the BcMYB51-3 promoter, which was jointly confirmed by Y1H, dual-LUC, and EMSA assays. The importance of MYB51, the homolog of BcMYB51-3, in the BcWRKY33A-mediated B. cinerea resistance was also verified using the TRV-based VIGS system. Overall, our data concludes that BcWRKY33A directly activates the expression of BcMYB51-3 and downstream IGSs’ biosynthetic genes, thereby improving the B. cinerea tolerance of NHCC plants.

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Changes in glucosinolates content in Brassica oleracea modulate disease severity caused by Xanthomonas campestris pv. campestris

Cited by 2 publications

References 8 publications

Glucosinolate Profile and Glucosinolate Biosynthesis and Breakdown Gene Expression Manifested by Black Rot Disease Infection in Cabbage

Glucosinolate Profile and Glucosinolate Biosynthesis and Breakdown Gene Expression Manifested by Black Rot Disease Infection in Cabbage

BcWRKY33A Enhances Resistance to Botrytis cinerea via Activating BcMYB51-3 in Non-Heading Chinese Cabbage

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