Changes in gill H+-ATPase and Na+/K+-ATPase expression and activity during freshwater acclimation of Atlantic salmon (<i>Salmo salar</i>)

Bystriansky, Jason S.; Schulte, Patricia M.

doi:10.1242/jeb.050633

Cited by 70 publications

(34 citation statements)

References 55 publications

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“…Nevertheless, when a fish experiences a change in external osmolarity, the expression level is expected to shift in order to handle the novel osmotic and ionic stress: it is therefore surprising that we see so little change in the overall expression on the shorter time-scale in this study. Overall, the main finding of ATP1A3 being less expressed in freshwater is in accordance with most research on salmonid fish, where gill Na + /K + -ATPase activity is higher in seawater acclimated fish and decreases following migration into freshwater [36], [37]; other fish species also show this pattern, including sea bass, Dicentrarchus labrax [38] and flounder, Platichthys flesus [39]. However, yet other studies have shown that the expression levels for the equivalent ATP isoform in Atlantic salmon ( Salmo salar ) did not change as a result of freshwater exposure [40], or as in killifish, where ATP1A3 was up-regulated in freshwater [21].…”

Section: Discussionsupporting

confidence: 88%

Small Changes in Gene Expression of Targeted Osmoregulatory Genes When Exposing Marine and Freshwater Threespine Stickleback (Gasterosteus aculeatus) to Abrupt Salinity Transfers

et al. 2014

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Salinity is one of the key factors that affects metabolism, survival and distribution of fish species, as all fish osmoregulate and euryhaline fish maintain osmotic differences between their extracellular fluid and either freshwater or seawater. The threespine stickleback (Gasterosteus aculeatus) is a euryhaline species with populations in both marine and freshwater environments, where the physiological and genomic basis for salinity tolerance adaptation is not fully understood. Therefore, our main objective in this study was to investigate gene expression of three targeted osmoregulatory genes (Na+/K+-ATPase (ATPA13), cystic fibrosis transmembrane regulator (CFTR) and a voltage gated potassium channel gene (KCNH4) and one stress related heat shock protein gene (HSP70)) in gill tissue from marine and freshwater populations when exposed to non-native salinity for periods ranging from five minutes to three weeks. Overall, the targeted genes showed highly plastic expression profiles, in addition the expression of ATP1A3 was slightly higher in saltwater adapted fish and KCNH4 and HSP70 had slightly higher expression in freshwater. As no pronounced changes were observed in the expression profiles of the targeted genes, this indicates that the osmoregulatory apparatuses of both the marine and landlocked freshwater stickleback population have not been environmentally canalized, but are able to respond plastically to abrupt salinity challenges.

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Section: Discussionsupporting

confidence: 88%

Small Changes in Gene Expression of Targeted Osmoregulatory Genes When Exposing Marine and Freshwater Threespine Stickleback (Gasterosteus aculeatus) to Abrupt Salinity Transfers

et al. 2014

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“…This pattern is different from that of the spotted green pufferfish, where higher levels (mRNA and protein) of branchial FXYD9 were found in FW-acclimated individuals as compared with SW-acclimated fish [32]. In time-course experiments performed with the Atlantic salmon, however, the expression of branchial fxyd9 showed no differences between time-points after the fish were transferred from FW to SW and vice versa [35], [36]. In the gills of the Mozambique tilapia, fxyd9 levels increased when the fish were transferred from SW to FW and rose quickly and then recovered gradually upon transfer from FW to SW [37].…”

Section: Discussioncontrasting

confidence: 59%

“…It has recently become clear that mammalian FXYD proteins modulate NKA activity [24], [26]–[28]. To date, few studies have investigated the salinity-dependent expression of FXYD proteins in teleosts, and these studies have focused on a rather limited group of species, including the euryhaline pufferfish [32] and tilapia [37], the anadromous salmon [34]–[36], the catadromous eel [38], and the stenohaline zebrafish [33]. In this study, we revealed similar and different expression of branchial fxyd genes in two closely related medaka species.…”

Section: Discussionmentioning

confidence: 99%

Expression Profiles of Branchial FXYD Proteins in the Brackish Medaka Oryzias dancena: A Potential Saltwater Fish Model for Studies of Osmoregulation

et al. 2013

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FXYD proteins are novel regulators of Na+-K+-ATPase (NKA). In fish subjected to salinity challenges, NKA activity in osmoregulatory organs (e.g., gills) is a primary driving force for the many ion transport systems that act in concert to maintain a stable internal environment. Although teleostean FXYD proteins have been identified and investigated, previous studies focused on only a limited group of species. The purposes of the present study were to establish the brackish medaka (Oryzias dancena) as a potential saltwater fish model for osmoregulatory studies and to investigate the diversity of teleostean FXYD expression profiles by comparing two closely related euryhaline model teleosts, brackish medaka and Japanese medaka (O. latipes), upon exposure to salinity changes. Seven members of the FXYD protein family were identified in each medaka species, and the expression of most branchial fxyd genes was salinity-dependent. Among the cloned genes, fxyd11 was expressed specifically in the gills and at a significantly higher level than the other fxyd genes. In the brackish medaka, branchial fxyd11 expression was localized to the NKA-immunoreactive cells in gill epithelia. Furthermore, the FXYD11 protein interacted with the NKA α-subunit and was expressed at a higher level in freshwater-acclimated individuals relative to fish in other salinity groups. The protein sequences and tissue distributions of the FXYD proteins were very similar between the two medaka species, but different expression profiles were observed upon salinity challenge for most branchial fxyd genes. Salinity changes produced different effects on the FXYD11 and NKA α-subunit expression patterns in the gills of the brackish medaka. To our knowledge, this report is the first to focus on FXYD expression in the gills of closely related euryhaline teleosts. Given the advantages conferred by the well-developed Japanese medaka system, we propose the brackish medaka as a saltwater fish model for osmoregulatory studies.

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“…Similar changes in Na + /K + -ATPase mRNA expression in response to salinity acclimation have been observed in the teleost gill, the analogous salt secreting tissue in these taxa (e.g. [24, 25], and [26]: meta-analysis of 59 studies including other taxa). With regards to Na + /K + -ATPase mRNA expression in the elasmobranch gill, in the euryhaline D. sabina and C. leucas , increased expression of gill Na + /K + -ATPase mRNA was observed in fresh water vs. seawater [15, 16].…”

Section: Resultssupporting

confidence: 53%

Na+/K+-ATPase α1 mRNA expression in the gill and rectal gland of the Atlantic stingray, Dasyatis sabina, following acclimation to increased salinity

Evans

Lambert

2015

BMC Res Notes

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BackgroundThe salt-secreting rectal gland plays a major role in elasmobranch osmoregulation, facilitating ion balance in hyperosmotic environments in a manner analogous to the teleost gill. Several studies have examined the central role of the sodium pump Na+/K+-ATPase in osmoregulatory tissues of euryhaline elasmobranch species, including regulation of Na+/K+-ATPase activity and abundance in response to salinity acclimation. However, while the transcriptional regulation of Na+/K+-ATPase in the teleost gill has been well documented the potential for mRNA regulation to facilitate rectal gland plasticity during salinity acclimation in elasmobranchs has not been examined. Therefore, in this study we acclimated Atlantic stingrays, Dasyatis sabina (Lesueur) from 11 to 34 ppt salinity over 3 days, and examined changes in plasma components as well as gill and rectal gland Na+/K+-ATPase α1 (atp1a1) mRNA expression.ResultsAcclimation to increased salinity did not affect hematocrit but resulted in significant increases in plasma osmolality, chloride and urea. Rectal gland atp1a1 mRNA expression was higher in 34 ppt-acclimated D. sabina vs. controls. There was no significant change in gill atp1a1 mRNA expression, however mRNA expression of this gene in the gill and rectal gland were negatively correlated.ConclusionsThis study demonstrates regulation of atp1a1 in the elasmobranch salt-secreting gland in response to salinity acclimation and a negative relationship between rectal gland and gill atp1a1 expression. These results support the hypothesis that the gill and rectal gland play opposing roles in ion balance with the gill potentially facilitating ion uptake in hypoosmotic environments. Future studies should further examine this possibility as well as potential differences in the regulation of Na+/K+-ATPase gene expression between euryhaline and stenohaline elasmobranch species.

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Changes in gill H+-ATPase and Na+/K+-ATPase expression and activity during freshwater acclimation of Atlantic salmon (Salmo salar)

Cited by 70 publications

References 55 publications

Small Changes in Gene Expression of Targeted Osmoregulatory Genes When Exposing Marine and Freshwater Threespine Stickleback (Gasterosteus aculeatus) to Abrupt Salinity Transfers

Small Changes in Gene Expression of Targeted Osmoregulatory Genes When Exposing Marine and Freshwater Threespine Stickleback (Gasterosteus aculeatus) to Abrupt Salinity Transfers

Expression Profiles of Branchial FXYD Proteins in the Brackish Medaka Oryzias dancena: A Potential Saltwater Fish Model for Studies of Osmoregulation

Na+/K+-ATPase α1 mRNA expression in the gill and rectal gland of the Atlantic stingray, Dasyatis sabina, following acclimation to increased salinity

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