Changes in gene transcription and taxane production in elicited cell cultures of Taxus×media and Taxus globosa

Ramírez-Estrada, Karla; Osuna, Lidia; Moyano, Encarnación; Bonfill, Mercedes; Tapia, Nadia; Cusidó, Rosa M.; Palazón, Javier

doi:10.1016/j.phytochem.2015.06.013

Cited by 46 publications

(30 citation statements)

References 41 publications

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“…4), MBCD, either alone or in combination with fungal elicitors, induced a high secretion, whereas control and fungal elicitor-treated CSC maintained more than 50% of produced paclitaxel inside C. avellana cells. Facilitating paclitaxel secretion from cells into the medium in MBCD-pretreated CSCs was reported in previous studies 40,42 . MBCD, through the chemical structure, facilitates paclitaxel secretion from cells into the medium and mitigates feedback inhibition.…”

Section: Effects Of Cyclodextrin and Fungal Elicitors On Paclitaxel Bsupporting

confidence: 61%

“…These results show a synergistic effect of MBCD and CW on paclitaxel biosynthesis in C. avellana cell culture. The previous studies reported the synergistic effect of MBCD and methyl jasmonate 42 or coronatine 40 on paclitaxel biosynthesis in Taxus cell culture, but this is the first report on the synergistic effect of MBCD and fungal elicitor (CW) on paclitaxel biosynthesis. Intra-and extracellular paclitaxel of MBCD-pretreated CSC exposed with 2.5% (v/v) of C. palmarum CW on 17 th day of cell culture cycle were 86.0 μg L −1 (1.8-fold) and 316.5 μg l −1 (14-fold), respectively.…”

Section: Effects Of Concentration Level and Adding Time Of Fungal Elimentioning

confidence: 88%

“…Cyclodextrin has recently absorbed remarkable attention not only as an agent inducing the biosynthesis of secondary metabolites in plant cell cultures, the consequence of defense response induction, but also for its capability to constitute the inclusion complexes with poorly water-soluble apolar compounds and facilitate the secretion of metabolites from cell to culture medium, thus act as a genuine elicitor [39][40][41] . Some studies 40,42,43 have been indicated that Methyl-β-Cyclodextrin (MBCD) enhanced paclitaxel biosynthesis, and also its secretion from cells to culture medium in Taxus cell culture. Therefore, the exploration of the combined effect of fungal elicitors with the elicitor/secretion activator MBCD on the biosynthesis and secretion of paclitaxel in C. avellana CSC is considered as crucial.…”

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confidence: 99%

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Fungal Cell Wall and Methyl-β–Cyclodextrin Synergistically Enhance Paclitaxel Biosynthesis and Secretion in Corylus avellana Cell Suspension Culture

Farhadi

Moieni

Safaie

et al. 2020

Sci Rep

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paclitaxel is the top-selling chemotherapeutic drug used for the treatment of lung, ovarian and breast cancer as well as Kaposi's sarcoma. cell suspension culture (cSc) of Corylus avellana has been addressed as a promising alternative for producing paclitaxel. in this study, endophytic fungus strain Yef 33 was isolated from Taxus baccata and identified as Coniothyrium palmarum. The effects of the elicitors derived from this fungus including cell extract, culture filtrate and cell wall (CW) and also chitin, alone or in combination with Methyl-β-cyclodextrin (MBcD), on paclitaxel biosynthesis in C. avellana cSc were assayed for the first time. CW of C. palmarum was the most efficient fungal elicitor for paclitaxel biosynthesis in C. avellana CSC. The results revealed that MBCD affected paclitaxel biosynthesis differently depending on fungal elicitor type and vice versa. MBCD, either alone or in combination with fungal elicitors, induced a high secretion of paclitaxel, suggesting the decrement of toxicity and retroinhibition processes of paclitaxel for cells. The joint effects of C. palmarum CW (2.5% (v/v) on 17 th day) and 50 mM MBCD synergistically enhanced paclitaxel biosynthesis (402.4 µg l −1 ; 5.8-fold), 78.6% of which (316.5 µg l −1) were secreted into culture medium, a level 146% higher than that in control. Paclitaxel, the most effective chemotherapy agent against lung, ovarian and breast cancer, and also Kaposi's sarcoma 1 , was originally extracted from Taxus brevifolia bark in 1967 2 and its structure was published in 1971 3 , and then it was joined the drug development program of National Cancer Institute (NCI) 4. Since the bark harvest is mortal for the trees, T. brevifolia was set on the endangered species list 4,5. Plant cell suspension culture (CSC) is a hopeful and nature-friendly system to mass production of paclitaxel 6-8. The worldwide demand for paclitaxel is rising at a high speed and its biosynthesis via Taxus cell factories is inadequate to handle the growing need of this medicine, mostly because of Taxus recalcitrant manner under in vitro conditions 6,7,9,10. Thus, finding the alternative sources of this valuable secondary metabolite is prompted. Corylus avellana, common hazel, has likewise been reported as a paclitaxel-producing species among angiosperms 6,7,10-15. The major superiority of producing paclitaxel by C. acellana cell culture is that in vitro culture of C. avellana is more facile than that of Taxus 6,7,9,12,16,17. In vitro culture of C. avellana has been reported as a hopeful and inexpensive method for producing paclitaxel 6,7,10,12,18. High-yielding in vitro culture setup is essential for producing secondary metabolites through plant cell culture 19. Bioactive compounds are usually fluctuated quantitatively/qualitatively under different conditions either in vivo or in vitro 6,7,10,12,20-23. Even engineered plant cells for overexpressing key genes still need using the elicitors for mass-biosynthesis of relevant secondary metabolite. Thus, screening the efficient elicitors for s...

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Section: Effects Of Cyclodextrin and Fungal Elicitors On Paclitaxel Bsupporting

confidence: 61%

Section: Effects Of Concentration Level and Adding Time Of Fungal Elimentioning

confidence: 88%

mentioning

confidence: 99%

See 1 more Smart Citation

Fungal Cell Wall and Methyl-β–Cyclodextrin Synergistically Enhance Paclitaxel Biosynthesis and Secretion in Corylus avellana Cell Suspension Culture

Farhadi

Moieni

Safaie

et al. 2020

Sci Rep

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“…Therefore, both Cor and methyl jasmonate produced a synergistic effect on trans-R biosynthesis in the presence of CD indicating that Cor was able to mimic the biologically active form of jasmonic acid, methyl jasmonate. A synergistic effect of CD and Cor on taxane production was also observed by Ramirez-Estrada et al (2015) in Taxus SCC. In fact, the combined treatment provoked an increase over 40-and 17.6-fold higher than in the control cells in Taxus media and T. globosa SCC, respectively.…”

Section: The Extracellular and Intracellular Production Of Trans-resvmentioning

confidence: 64%

“…This fact was also observed by Lijavetzky et al (2008) who showed that STS expression was highly induced by methyl jasmonate but no significant amounts of extracellular trans-R were detected. Similar studies were performed by Ramirez-Estrada et al (2015) who analyzed the expression pattern of genes in Taxus SCC elicited with Cor and CD separately or in combination. In agreement with our results, these authors also observed that despite the fact that the expression of the two genes encoding enzymes directly responsible for the formation of taxol was higher in the cell cultures treated only with Cor, the highest production of this taxane was obtained under CD plus Cor elicitation.…”

Section: Cell Growth Is Dependent On the Presence Of Elicitorsmentioning

confidence: 65%

Enhanced extracellular production of trans-resveratrol in Vitis vinifera suspension cultured cells by using cyclodextrins and coronatine

Almagro

Belchí-Navarro

Martínez-Márquez

et al. 2015

Plant Physiology and Biochemistry

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In the present work the effect of cyclodextrin and coronatine on both trans-resveratrol production and the expression of stilbene biosynthetic genes in Vitis vinifera L. cv Monastrell suspension cultured cells were evaluated. The results showed the maximum level of trans-resveratrol produced by cells and secreted to the culture medium with 50 mM cyclodextrins and 1 μM coronatine. Since the levels of trans-resveratrol produced in the combined treatment were higher than the sum of the individual treatments, a synergistic effect between both elicitors was assumed. In addition, all the analysed genes were induced by cyclodextrins and/or coronatine. The expression of the phenylalanine ammonia lyase and stilbene synthase genes was greatly enhanced by coronatine although an increase in the amount of trans-resveratrol in the spent medium was not detected. Therefore, despite the fact that trans-resveratrol production is related with the expression of genes involved in the biosynthetic process, other factors may be involved, such as post-transcriptional and post-traductional regulation. The expression maximal levels of cinnamate 4-hydroxylase and 4-coumarate-CoA ligase genes were found with cyclodextrins alone or in combination with coronatine suggesting that the activity of these enzymes could be not only important for the formation of intermediates of trans-R biosynthesis but also for those intermediates involved in the biosynthesis of lignins and/or flavonoids.

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Recent Trends in Elicitation and Secondary Metabolic Pathway Analysis in Medicinal Plants: An Integrated Transcriptomics and Proteomics Approach

Giri

2022

Medicinal and Aromatic Plants of the World

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Changes in gene transcription and taxane production in elicited cell cultures of Taxus×media and Taxus globosa

Cited by 46 publications

References 41 publications

Fungal Cell Wall and Methyl-β–Cyclodextrin Synergistically Enhance Paclitaxel Biosynthesis and Secretion in Corylus avellana Cell Suspension Culture

Fungal Cell Wall and Methyl-β–Cyclodextrin Synergistically Enhance Paclitaxel Biosynthesis and Secretion in Corylus avellana Cell Suspension Culture

Enhanced extracellular production of trans-resveratrol in Vitis vinifera suspension cultured cells by using cyclodextrins and coronatine

Recent Trends in Elicitation and Secondary Metabolic Pathway Analysis in Medicinal Plants: An Integrated Transcriptomics and Proteomics Approach

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