Changes in gene position are accompanied by a change in time of replication

Calza, R. E.; Eckhardt, Laurel A.; DelGiudice, T.; Schildkraut, Carl L.

doi:10.1016/0092-8674(84)90349-0

Cited by 140 publications

(86 citation statements)

References 22 publications

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“…As noted in the introduction, analysis of replication timing in the mouse plasmacytoma cell line MPC11 implicated the region downstream of the Igh locus as responsible for early replication (5). Furthermore, the same general region is implicated as an origin of Igh DNA replication in MEL, as inferred from the work of Brown et al (4).…”

Section: Isolation Of Sequences Downstream Of the Igh 3 Regulatory Rementioning

confidence: 57%

“…In contrast, the C␥2b gene, which is dissociated from the downstream portion of the Igh gene cluster as a result of its juxtaposition with c-myc, replicated late in S phase. The reciprocal translocation product, which contains myc gene segments associated with downstream Igh sequences, replicated earlier than myc sequences on the unrearranged chromosome 15 (5). These studies implicated the presence of regulatory regions located downstream of the Igh gene cluster which control early replication of associated sequences in B cells.…”

mentioning

confidence: 75%

“…For MEL, five separate transfers were hybridized, and a representative blot is shown. Blots used for panels B and C were prepared as described elsewhere (4,5). Probes used are as indicated.…”

Section: Discussionmentioning

confidence: 99%

“…The implication from the altered timing of replication of the C␥2b gene as a result of the MPC11 translocation was that sequences in the 3Ј region of the Igh locus might control replication timing in B cells (5). To test whether the Igh 3Ј regulatory region might be responsible for directing early replication of the Igh locus in B-lymphoid lines, we took advantage of the mutant myeloma cell line LP1.2 (low producing).…”

Section: Isolation Of Sequences Downstream Of the Igh 3 Regulatory Rementioning

confidence: 99%

“…In MPC11, as in other plasmacytomas, in addition to the expressed Igh locus on chromosome 12, there is a characteristic chromosomal translocation involving the Igh locus and the myc locus from chromosome 15. Igh sequences in the different chromosomal contexts exhibited dramatic differences in replication timing (5). The productively rearranged C␥2b gene (chromosome 12) replicated early in S phase.…”

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confidence: 99%

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Regulation of the Replication of the Murine Immunoglobulin Heavy Chain Gene Locus: Evaluation of the Role of the 3′ Regulatory Region

Michaelson

Ermakova

Birshtein

et al. 1997

Molecular and Cellular Biology

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DNA replication in mammalian cells is a precisely controlled physical and temporal process, likely involving cis-acting elements that control the region(s) from which replication initiates. In B cells, previous studies showed replication timing to be early throughout the immunoglobulin heavy chain (Igh) locus. The implication from replication timing studies in the B-cell line MPC11 was that early replication of the Igh locus was regulated by sequences downstream of the C␣ gene. A potential candidate for these replication control sequences was the 3 regulatory region of the Igh locus. Our results demonstrate, however, that the Igh locus maintains early replication in a B-cell line in which the 3 regulatory region has been deleted from one allele, thus indicating that replication timing of the locus is independent of this region. The faithful duplication of the mammalian genome by DNA replication occurs in a physically and temporally ordered fashion during each round of S phase. In contrast to Escherichia coli, in which bidirectional DNA replication of a circular chromosome usually initiates at a single defined origin of replication (10, 26), the significantly larger genome in higher organisms requires DNA synthesis to initiate at multiple origins. Questions regarding where replication initiates and what controls replication initiation are fundamental in understanding the ordered program of DNA replication.DNA replication in eukaryotes does not initiate from all origins simultaneously; rather, replication origins fire in a programmed manner at fixed intervals during S phase. A relationship between transcriptional activity and early timing of replication has been observed, suggestive of potential coregulation of the two processes. Housekeeping genes, accordingly, replicate relatively early in S phase in most cell types (34). Tissuespecific genes also generally replicate early in S phase in cell types in which they are expressed; however, they often replicate late when they are not transcribed. The ␤-globin locus, for example, is early replicating in murine erythroleukemia cells (MEL) and late replicating in other cell types, such as lymphocytes (13, 18). Significantly, the same origin, located between the ␦-and ␤-globin genes, is utilized in both erythroleukemia cells and lymphocytes (references 1 and 20 and references therein).For the mouse immunoglobulin heavy chain (Igh) locus, the temporal order of Igh gene replication has been assessed by measuring the relative concentrations of EcoRI restriction fragment segments in 5-bromouracil-labeled DNA (BU-DNA) during various intervals of S phase (4). The murine Igh locus is comprised of variable region genes, including V (variable), D (diversity), and J (joining) segments, and constant region genes. (The Igh genes are arranged on chromosome 12 as follows: telomere-V-D-J-C H -centromere. The terms "downstream" and "3Ј" are used in this article with reference to the transcriptional orientation of the Igh genes in B cells.) In non-B cells, such as MEL, the 3Ј-most constan...

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Section: Isolation Of Sequences Downstream Of the Igh 3 Regulatory Rementioning

confidence: 57%

mentioning

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Section: Isolation Of Sequences Downstream Of the Igh 3 Regulatory Rementioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Regulation of the Replication of the Murine Immunoglobulin Heavy Chain Gene Locus: Evaluation of the Role of the 3′ Regulatory Region

Michaelson

Ermakova

Birshtein

et al. 1997

Molecular and Cellular Biology

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The S phase: Beginning, middle, and end: A perspective

Ford¹,

Pardee²

1998

J. Cell. Biochem.

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Events in the S phase of the cell cycle have been investigated to a relatively limited extent in comparison with those in G1 and M phases. Four aspects of S are briefly discussed in this report: (1) the final biochemical step permitting initiation of DNA synthesis, (2) determination of replication timing of individual genes and its mechanism, (3) S phase processes that lead to the onset of M phase, and (4) resetting the S-phase machinery. J. Cell. Biochem. Suppls. 30/31:1-7, 1998. © 1999 Wiley-Liss, Inc.

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