Changes in gene expression profiles of bovine embryos produced in vitro, by natural ovulation, or hormonal superstimulation

Mundim, Tatiane Carmo Duarte; Ramos, A. F.; Sartori, Roberto; Dode, M. A. N.; Melo, E. O.; Gomes, Latini; Rumpf, R.; Franco, M. M.

doi:10.4238/vol8-4gmr646

Cited by 45 publications

(36 citation statements)

References 41 publications

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“…5) were activated in granulosa cells from superstimulated follicles. Our results are supported by similar findings in embryos from superstimulated donors (Mundim et al 2009), where genes related to oxidative stress response tended to be activated. In this study, the NFE2L2 oxidative stress response pathway was one of the most activated pathways in the superstimulation group.…”

Section: The Effect Of Superstimulation On Folliclessupporting

confidence: 90%

“…The effect of superstimulation treatment on the follicular environment and oocyte quality is also not well understood. Previous studies suggest that superstimulation can trigger genes related to the oxidative stress response of embryos of mice (Rossignol et al 2006, Fauque et al 2007, Sato et al 2007) and cattle (Mundim et al 2009). However, these studies involved analysis of the genetic status of embryos and tested only a few genes.…”

Section: Introductionmentioning

confidence: 99%

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Differential gene expression of granulosa cells after ovarian superstimulation in beef cattle

Dias¹,

Khan²,

Sirard³

et al. 2013

Reproduction

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Microarray analysis was used to compare the gene expression of granulosa cells from dominant follicles with that of those after superstimulatory treatment. Cows were allocated randomly to two groups (superstimulation and control, nZ6/group). A new follicular wave was induced by ablation of follicles R5 mm in diameter, and a progesterone-releasing device controlled internal drug release (CIDR) was placed in the vagina. The superstimulation group was given eight doses of 25 mg FSH at 12-h intervals starting from the day of wave emergence (day 0), whereas the control group was not given FSH treatment. Both groups were given prostaglandin F 2a twice, 12 h apart, on day 3 and the CIDR was removed at the second injection; 25 mg porcine luteinizing hormone (pLH) was given 24 h after CIDR removal, and cows were ovariectomized 24 h later. Granulosa cells were collected for RNA extraction, amplification, and microarray hybridization. A total of 190 genes were downregulated and 280 genes were upregulated. To validate the microarray results, five genes were selected for real-time PCR (NTS, FOS, THBS1, FN1, and IGF2). Expression of four genes increased significantly in the three different animals tested (NTS, FOS, THBS1, and FN1). The upregulated genes are related to matrix remodeling (i.e. tissue proliferation), disturbance of angiogenesis, apoptosis, and oxidative stress response. We conclude that superstimulation treatment i) results in granulosa cells that lag behind in maturation and differentiation (most of the upregulated genes are markers of the follicular growth stage), ii) activates genes involved with the NFE2L2 oxidative stress response and endoplasmic reticulum stress response, and iii) disturbs angiogenesis.

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Section: The Effect Of Superstimulation On Folliclessupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Differential gene expression of granulosa cells after ovarian superstimulation in beef cattle

Dias¹,

Khan²,

Sirard³

et al. 2013

Reproduction

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“…In several studies, mRNA expression levels of BAX have been used to evaluate embryo quality developed in vitro (Amarnath et al 2007;Mundim et al 2009;Shiqiang et al 2010;Kim et al 2012;Sadeesh et al 2015b). Data from the current study indicate that BAX expression was greater (P \ 0.05) in embryos produced from undefined media-containing serum than those produced in its absence, suggesting that over expression of the BAX gene, in the cellular apoptotic pathway takes place (Yang and Rajamahendran 2002;Opiela et al 2008).…”

Section: Discussionmentioning

confidence: 53%

Differences in developmental competence and gene expression profiles between buffalo (Bubalus bubalis) preimplantation embryos cultured in three different embryo culture media

et al. 2016

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The objective of this study was to compare effects of in vitro culture systems on embryonic development and expression patterns of developmentally important genes in preimplantation buffalo embryos. After IVM/IVF presumptive zygotes were cultured in one of three systems: undefined TCM-199, mCR2aa medium supplemented with 10 % FBS and defined PVA-myo-inositol-phosphate-EGF medium. No (P [ 0.05) differences at 2-cell, 4-cell and 8-cell to 16-cell stages were observed among the three cultured media used, however, increased (P \ 0.05) blastocyst yield, cell number and hatching rate were found in defined medium compared to undefined media. The expression patterns of genes implicated in embryo metabolism (GLUT-1), anti-apoptosis (BCL-2), imprinting (IGF-2R), DNA methylation (DNMT-3A) and maternal recognition of pregnancy (IFNT) were increased (P \ 0.05) in hatched blastocysts derived from defined medium compared to undefined media. In conclusion, serum-free, defined medium improved developmental competence of in vitro cultured buffalo embryos. Whether these differences in morphological development and gene expression have long-term effects on buffalo calves born after embryo transfer remains unknown. However, it is possible that early adaptations of the preimplantation embryo to its environment persist during fetal and post-natal development.

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“…Oocytes that are normally regressed or are still too immature to ovulate are forcibly ovulated by hormonal stimulation. These oocytes do not contain the proper maternal factors, mRNA, proteins, and mature mitochondria (Krisher 2004, Thouas et al 2004, Cui & Kim 2007, Hamatani et al 2008, Mtango et al 2008, Mundim et al 2009, Wai et al 2010, Eichenlaub-Ritter et al 2011. Therefore, we considered that some IVF embryos that are derived from superovulated oocytes may contain mitochondrial functional defects or not enough maternal factors to upregulate the DJ m .…”

Section: Female Nomentioning

confidence: 99%

“…These superovulated oocytes are generally considered to contain some oocytes that would normally be degraded before spontaneous ovulation or that are still immature during ovulation so that some oocytes may not store enough maternal mRNA and proteins. Hormonal stimulation for superovulation influences maternal mRNA gene expression (Mundim et al 2009). In addition to maternal mRNA and proteins, mitochondria are inherited to oocytes as the maternal factor (Sato & Sato 2011).…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial membrane potential in 2-cell stage embryos correlates with the success of preimplantation development

Komatsu¹,

Iwase²,

Mawatari³

et al. 2014

Reproduction

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Hormonal stimulation in superovulation induces female mice to ovulate more oocytes than spontaneous ovulation. Because the superovulated oocytes contain a number of oocytes that normally regress before spontaneous ovulation or immature oocytes, the development of some embryos that derive from these oocytes by IVF is prevented. Therefore, the quality of superovulated oocytes should differ from that of spontaneously ovulated oocytes. In this study, we evaluated the quality of superovulated oocytes, by examining 1-and 2-cell stage embryos, in which the development mainly depends on the maternal mRNA, proteins, and mitochondria that are contained in the oocytes, and we then measured the mitochondrial membrane potential (DJ m ) of the 1-and 2-cell stage, in vivo-fertilized, and IVF embryos. The DJ m of 1-cell stage IVF embryos was lower than that of in vivo-fertilized embryos; however, there was no difference between IVF embryos. During the developmental process from 1-to 2-cell stage, the DJ m of in vivo-fertilized embryos was highly upregulated, whereas a number of IVF embryos remained unchanged. As a result, 2-cell stage embryos were divided into two groups: high-and low-DJ m 2-cell stage IVF embryos. The development of low-DJ m 2-cell stage IVF embryos tended to be arrested after the 2-cell stage. These results indicated that the upregulation of DJ m during the 1-to 2-cell stage was important in the development of early preimplantation embryos; there were some defects in the mitochondria of superovulated oocytes, which prevented their development.

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Changes in gene expression profiles of bovine embryos produced in vitro, by natural ovulation, or hormonal superstimulation

Cited by 45 publications

References 41 publications

Differential gene expression of granulosa cells after ovarian superstimulation in beef cattle

Differential gene expression of granulosa cells after ovarian superstimulation in beef cattle

Differences in developmental competence and gene expression profiles between buffalo (Bubalus bubalis) preimplantation embryos cultured in three different embryo culture media

Mitochondrial membrane potential in 2-cell stage embryos correlates with the success of preimplantation development

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