Changes in French bean cotyledon composition associated with modulated life-span

Wilhelmová, N.; Wilhelm, J.; Kutík, J.; Haisel, D.

doi:10.1023/a:1006859917345

Cited by 13 publications

(6 citation statements)

References 22 publications

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“…The latter have been mostly reported in a context with relatively high irradiance, which induces leaves senescence (Biswal and Biswal 1984, Noodén et al 1996, Weaver and Amasino 2001. In our experiments cotyledon senescence induced by continuous irradiation occurred earlier than natural senescence: the life span of cotyledons of C plants was 16 d, of CL plants only 13 d. Chlorophyll (Chl) content is a generally used marker of leaf senescence (Thimann 1980, Matile 1992, Hillman et al 1994, Wilhelmová et al 1997. It can be assumed that the onset of senescence occurred after the 13 th day in C plants because from the 13 th day both Chl content and photochemical efficiency (expressed as F v /F m ratio) decreased.…”

Section: Discussionmentioning

confidence: 71%

“…Jantar) plants were grown in sand in plastic pots in a growth chamber (Klimabox 1300, Kladno, Czech Republic). The control plants (C plants) were grown under 16-h photoperiod, temperature 24/18 °C, air humidity 60/80 % and irradiation of 220 µmol(photon) m -2 s -1 (for detail see Wilhelmová et al 1997). Continuously irradiated plants (CL plants) were grown under continuous irradiation 220 µmol m -2 s -1 , temperature 24 °C and air humidity 60 %.…”

Section: Methodsmentioning

confidence: 99%

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Changes in Antioxidative Protection in Bean Cotyledons During Natural and Continuous Irradiation-Accelerated Senescence

Procházková

Wilhelmová

2004

Biol. plant.

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We employed continuous irradiation (CL) for induction of premature senescence caused by enhanced production of reactive oxygen species. As a model plant we used bean (Phaseolus vulgaris L. cv. Jantar) cotyledons because they have well defined and a quite short life span. Senescence of bean cotyledons induced by CL progressed more rapidly than natural senescence: the life span of CL cotyledons was 13 d compared to 16 d in controls (C). Chl content was significantly lower in 10-and 13-d-old CL plants than in C plants and the change with age was not statistically significant. Activities of all antioxidative enzymes declined either with senescence onset or during whole life span. Activity of antioxidative enzymes, except ascorbate peroxidase, was lower in CL plants compared to C plants. On the contrary, contents of non-enzymatic antioxidants β-carotene and ascorbate were higher in CL plants than in C plants. No significant difference, except in the youngest cotyledons, was observed in glutathione content.

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Section: Discussionmentioning

confidence: 71%

Section: Methodsmentioning

confidence: 99%

Changes in Antioxidative Protection in Bean Cotyledons During Natural and Continuous Irradiation-Accelerated Senescence

Procházková

Wilhelmová

2004

Biol. plant.

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“…LFP assay: LFP were estimated in lipophilic extracts of whole cotyledons in chloroform after HPLC (Shimadzu LC9, Kyoto, Japan) resolution. They were characterized by blue fluorescence emission after excitation in UV using fluorimeter Perkin Elmer LS5 (Boston, USA; Wilhelmová et al 1997). The fluorometer was calibrated with the standard No.…”

Section: Determination Of Cytokininsmentioning

confidence: 99%

“…In senescent cotyledons, the percentage of LFP decreased in cytoplasm and increased in microsomal membranes (Hudák et al 1995). We have characterized LFP in ageing of bean cotyledons and their time-course after decapitation of the plant epicotyl (Wilhelmová et al 1997).…”

Section: Introductionmentioning

confidence: 99%

The Role of Cytokinins and Ethylene in Bean Cotyledon Senescence. The Effect of Free Radicals

Wilhelmová¹,

Procházková²,

Macháčková³

et al. 2004

Biol. plant.

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During ageing of bean (Phaseolus vulgaris L.) cotyledons in plants with modified life span the time-course of four cytokinins, ethylene, and the end products of free radical attack, lipofuscin-like pigments (LFP), were studied. UV irradiation shortened cotyledon life span, while epicotyl decapitation prolonged it. In controls, LFP increased at the senescence onset but at the end of life span it returned to the initial level. Ethylene increased more than 3-fold at the time of abscission. The content of individual cytokinins (zeatin, zeatin riboside, isopentenyl adenine, isopentenyl adenine riboside) varied differently during ageing but they did not decreased in any case under level observed in young cotyledons at the time of abscission. UV irradiation resulted in 14-fold increase in LFP concentration at the end. Ethylene increased 8-fold 2 h after irradiation. Individual cytokinins increased after UV irradiation to a different extent and time-course, nevertheless cotyledon life span was shortened. Decapitation induced LFP decrease. On day 13, LFP abruptly increased and than decreased and stayed lowered until abscission. Ethylene was maximum on day 24, at the time of abscission, it was above 200 % of control. Decapitation produced transient decrease in some cytokinins namely zeatin and isopentenyl adenine riboside.

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“…These changes help in scavenging free radicals and harmful oxidants in plant cells, thereby protecting them from oxidative damage . Second, under conditions of limited soil moisture, plant metabolism undergoes changes at the developmental, physiological, and molecular levels, resulting in reduced photosynthetic rates, alterations in photosynthetic proton and electron transport, and decreased carbon oxidation and assimilation. , CKs have multiple effects on plant photosynthesis, and they can promote chloroplast differentiation, transformation, and division, increase the number of chloroplasts, promote the formation of grana, and elevate the levels of photosynthetic pigments such as chlorophyll and carotenoids. − Moreover, CKs can enhance photosynthetic rates, photochemical quenching, and the maximum photochemical efficiency of photosystem II . During drought stress, CKs can slow down the decline in chlorophyll content, the reduction in grana formation, and the decrease in photosynthetic parameters …”

Section: Introductionmentioning

confidence: 99%

Insights into the Impact of Trans-Zeatin Overproduction-Engineered Sinorhizobium meliloti on Alfalfa (Medicago sativa L.) Tolerance to Drought Stress

Yu,

Luo,

et al. 2024

J. Agric. Food Chem.

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Plant growth-promoting rhizobacteria have been shown to enhance plant tolerance to drought stress through various mechanisms. However, there is limited research on improving drought resistance in alfalfa by genetically modifying PGPR to produce increased levels of cytokinins. Herein, we employed synthetic biology approaches to engineer two novel strains of Sinorhizobium meliloti capable of overproducing trans-Zeatin and investigated their potential in enhancing drought tolerance in alfalfa. Our results demonstrate that alfalfa plants inoculated with these engineered S. meliloti strains exhibited reduced wilting and yellowing while maintaining higher relative water content under drought conditions. The engineered S. meliloti-induced tZ activated the activity of antioxidant enzymes and the accumulation of osmolytes. Additionally, the increased endogenous tZ content in plants alleviated the impact of drought stress on the alfalfa photosynthetic rate. However, under nondrought conditions, inoculation with the engineered S. meliloti strains had no significant effect on alfalfa biomass and nodule formation.

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Changes in French bean cotyledon composition associated with modulated life-span

Cited by 13 publications

References 22 publications

Changes in Antioxidative Protection in Bean Cotyledons During Natural and Continuous Irradiation-Accelerated Senescence

Changes in Antioxidative Protection in Bean Cotyledons During Natural and Continuous Irradiation-Accelerated Senescence

The Role of Cytokinins and Ethylene in Bean Cotyledon Senescence. The Effect of Free Radicals

Insights into the Impact of Trans-Zeatin Overproduction-Engineered Sinorhizobium meliloti on Alfalfa (Medicago sativa L.) Tolerance to Drought Stress

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