Changes in fatty acid composition associated with differentiation of Trypanosoma cruzi

Esteves, Manuel G.; Gonzales-Perdomo, Magaly; Alviano, Celuta Sales; Angluster, Jayme; Goldenberg, Samuel

doi:10.1111/j.1574-6968.1989.tb03077.x

Cited by 17 publications

(6 citation statements)

References 20 publications

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“…For some lectins, Ep-TAU resembled Mt much more than Ep-LIT, suggesting that changes in the surface sugar composition precede the morphological transformation of epimastigotes into metacyclic trypomastigotes. This is in agreement with previous observations concerning the fatty acid composition of differentiating epimastigotes (Esteves et al, 1989).…”

Section: Discussionsupporting

confidence: 83%

“…In addition to morphological changes, the metacyclogenesis process involves differential gene expression (Contreras et al, 1985;Bonaldo et al, 1988) and changes in fatty acid composition (Esteves et al, 1989). The process also involves changes in surface carbohydrates, since epimastigotes and trypomastigotes display different lectin agglutination profiles (Pereira et al, 1980).…”

Section: Introductionmentioning

confidence: 99%

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Changes in cell-surface carbohydrates of Trypanosoma cruzi during metacyclogenesis under chemically defined conditions

Andrade¹,

Esteves²,

Angluster³

et al. 1991

Journal of General Microbiology

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Highly purified lectins with specificities for receptor molecules containing sialic acid, N-acetylglucosamine (DGlcNAc), N-acetylgalactosamine (D-GalNAc), galactose (D-Gal), mannose-like residues (D-Man) or L-fucose (LFuc), were used to determine changes in cell-surface carbohydrates of the protozoal parasite Trypanosomu cruzi during metacyclogenesis under chemically defined conditions. Of the D-GalNAc-binding lectins, BS-I selectively agglutinated metacyclic trypomastigotes, MPL was selective for replicating epimastigotes, whereas SBA strongly agglutinated all developmental stages of T. cruzi. WGA (sialic acid and/or D-GIcNAc specific) was also reactive with differentiating epimastigotes and metacyclic trypomastigotes but displayed a higher reactivity with replicating epimastigote forms. A progressive decrease in agglutinating activity was observed for jacaline (specific for D-Gal) during the metacyclogenesis process; conversely, a progressive increase in affinity was observed for RCA-I (D-Gabspecific), although the reactivity of other D-Gal-specific lectins (PNA and AxP) was strong at all developmental stages. All developmental stages of T. cruzi were agglutinated by Con A and Lens cufinaris lectins (specific for D-Man-like residues); however, they were unreactive with the L-fucose-binding lectins from Lotus tetrugonolobos and Ufex europueus. These agglutination assays were further confirmed by binding studies using I z51-labelled lectins. Neuraminidase activity was detected in supernatants of cell-free differentiation medium using the PNA hemagglutination test with human A erythrocytes. The most pronounced differences in lectin agglutination activity were observed between replicating and differentiating epimastigotes, suggesting that changes in the composition of accessible cell-surface carbohydrates precede the morphological transformation of epimastigotes into metacyclic trypomastigotes.

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Section: Discussionsupporting

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Changes in cell-surface carbohydrates of Trypanosoma cruzi during metacyclogenesis under chemically defined conditions

Andrade¹,

Esteves²,

Angluster³

et al. 1991

Journal of General Microbiology

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“…The latter are known to suffer long periods of starvation, which can last for more than 12 months depending on species, instar, and climatic conditions (48). Furthermore, nutritionally poor growth media such as triatomine artificial urine and M16 induce metacyclogenesis, a process during which epimastigotes differentiate to metacyclic trypomastigotes and which occurs in nature in the insect rectum (49,50). The process, therefore, requires fast and extensive protein degradation and recycling of building blocks for the synthesis of new macromolecules.…”

Section: Discussionmentioning

confidence: 99%

Autophagy Is Involved in Nutritional Stress Response and Differentiation in Trypanosoma cruzi

Álvarez

Kosec

Sant’Anna

et al. 2008

Journal of Biological Chemistry

137

173

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Autophagy is the major mechanism used by eukaryotic cells to degrade and recycle proteins and organelles. Bioinformatics analysis of the genome of the protozoan parasite Trypanosoma cruzi revealed the presence of all components of the Atg8 conjugation system, whereas Atg12, Atg5, and Atg10 as the major components of the Atg12 pathway could not be identified. The two TcATG4 (autophagin) homologs present in the genome were found to correctly process the two ATG8 homologs after the conserved Gly residue. Functional studies revealed that both ATG4 homologues but only one T. cruzi ATG8 homolog (TcATG8.1) complemented yeast deletion strains. During starvation of the parasite, TcAtg8.1, but not TcAtg8.2, was found by immunofluorescence to be located in autophagosome-like vesicles. This confirms its function as an Atg8/LC3 homolog and its potential to be used as an autophagosomal marker. Most importantly, autophagy is involved in differentiation between developmental stages of T. cruzi, a process that is essential for parasite maintenance and survival. These findings suggest that the autophagy pathway could represent a target for a novel chemotherapeutic strategy against Chagas disease.

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“…Metacyclogenesis is the complex differentiation process from T. cruzi epimastigotes (E), proliferative and non-infectious forms, to metacyclic trypomastigotes (MT), nonproliferative and infective forms. This transformation involves proteolytic enzymes, and goes through alterations in protein N-glycosylation as well as changes of cell surface sugar (Engel and Parodi, 1985 ;De Andrade et al 1991 ;Bourguignon et al 1998) and lipid composition (Esteves et al 1989) upon differentiation. In addition, the development of the differential expression method has led to the cloning of Trypanosoma cruzi stage-specific genes (Avila et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Novel cysteine proteinase inTrypanosoma cruzimetacyclogenesis

et al. 2005

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S U M M A R YWith the aim to study proteinases released to the culture medium during Trypanosoma cruzi metacyclogenesis, the presence of cysteine proteinases (CPs) was analysed in culture supernatants obtained throughout the differentiation induced by stimulation of epimastigotes with Triatoma infestans hindgut homogenate. In SDS-gelatin containing gels, an important endopeptidase activity with apparent molecular weight range between 97 and 116 kDa was encountered at pH 6, which was abolished by the specific cysteine proteinase inhibitor E-64 and TLCK, but not by pepstatin, 1,10 phenantroline or PMSF. This novel CP, named TcCPmet, showed affinity to cystatin-Sepharose, denoting its thiol-proteinase character as well as to ConA-Sepharose, indicating it contains N-linked oligosaccharides. However, it presented a different elution pattern on ConA-Sepharose than cruzipain and, in addition, it was not recognized by anti-cruzipain serum, facts that strongly suggest the different nature of both CPs. Moroever, evidence is presented indicating that TcCPmet was able to hydrolyse the same chromogenic peptides as cruzipain at optimal alkaline pH values, although with a different order of effectiveness. Our results indicate the presence of a novel CP secreted by metacyclic trypomastigotes and reinforces the important role of these enzymes in metacyclogenesis.

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Changes in fatty acid composition associated with differentiation of Trypanosoma cruzi

Cited by 17 publications

References 20 publications

Changes in cell-surface carbohydrates of Trypanosoma cruzi during metacyclogenesis under chemically defined conditions

Changes in cell-surface carbohydrates of Trypanosoma cruzi during metacyclogenesis under chemically defined conditions

Autophagy Is Involved in Nutritional Stress Response and Differentiation in Trypanosoma cruzi

Novel cysteine proteinase inTrypanosoma cruzimetacyclogenesis

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