Changes in Extracellular Concentrations of Some Cytokines, Chemokines, and Neurotrophic Factors After Insertion of Intracerebral Microdialysis Catheters in Neurosurgical Patients

Mellergård, Pekka; Åneman, Oscar; Sjögren, Florence; Pettersson, Pernilla; Hillman, Jan

doi:10.1227/01.neu.0000311072.33615.3a

Cited by 58 publications

(75 citation statements)

References 43 publications

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“…We have previously shown significant and transient effects on extracellular interleukin concentrations associated with the insertion of a microdialysis catheter (Mellergard et al, 2008). In the present series of seriously injured NSICU patients with highly individual needs, the catheter insertion time could not be standardized.…”

Section: Sampling Of Microdialysatementioning

confidence: 80%

“…The insertion and handling of microdialysis catheters followed our normal routines as previously described in detail Mellergard et al, 2008). CMA71 catheters (with 100 kDa cut-off ) were perfused at a standardized flow of 0.3 mL/min with Ringer-Dextran 60 (RD60, Braun Medical, Stockholm, Sweden) or poly-(0-2-hydroxyethyl) starch in 0.9% sodium chloride (Voluven ª , Fresenius Kabi, Stockholm, Sweden), utilizing the CMA 106 perfusion pump (CMA Microdialysis, Stockholm, Sweden).…”

Section: Sampling Of Microdialysatementioning

confidence: 99%

“…The microdialysate was analyzed for the content of interleukin-1b (IL-1b), interleukin-6 (IL-6), interleukin-8 (IL-8), macrophage inflammatory protein-1b (MIP-1b), regulatedupon-activation normal T-cell expressed and secreted) (RANTES, also known as CCL-5), vascular endothelial growth factor (VEGF), and basic fibroblast growth factor (FGFb), with Fluorokine MAP Multiplex Assays for the Luminex platform (R&D Systems, Oxford, United Kingdom), a method based on multiplex bead technology, as previously described (Mellergard et al, 2008). Briefly, different populations of polystyrene beads (5.6 lm in diameter) are covalently coupled with an antibody directed against a specific cytokine.…”

Section: Sampling Of Microdialysatementioning

confidence: 99%

“…The study is part of an ongoing project, aimed at optimizing the care of NSICU patients (Mellergard et al, 2008(Mellergard et al, , 2010(Mellergard et al, , 2011.…”

Section: Introductionmentioning

confidence: 99%

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The Cerebral Extracellular Release of Glycerol, Glutamate, and FGF2 Is Increased in Older Patients following Severe Traumatic Brain Injury

Mellergård

Sjögren

Hillman

2012

Journal of Neurotrauma

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Old age is associated with a poor recovery from traumatic brain injury (TBI). In a retrospective study we investigated if the biochemical response following TBI is age dependent. Extracellular fluids were continuously sampled by microdialysis in 69 patients admitted to our NSICU following severe TBI. The concentrations of glycerol, glutamate, lactate, pyruvate, and eight different cytokines (IL-1b, IL-6, IL-10, IL-8, MIP-1b, RANTES, FGF2, and VEGF) were determined by fluorescence multiplex bead technology. Patients in the oldest age group ( ‡ 65 years) had significantly higher microdialysate concentrations of glycerol and glutamate compared to younger patients: the mean microdialysate concentration of glycerol increased from 55.9 lmol/L (25-44 year) to 252 lmol/L ( ‡ 65 years; p < 0.0001); similarly glutamate increased from 15.8 mmol/L to 92.2 mmol/L ( p < 0.0001). The lactate-pyruvate ratio was also significantly higher in the patients ‡ 65 years of age (63.9) compared with all the other age groups. The patterns of cytokine responses varied. For some cytokines (IL-1b, IL-10, and IL-8) there were no differences between age groups, while for others (MIP-1b, RANTES, VEGF, and IL-6) some differences were observed, but with no clear correlation with increasing age. For FGF2 the mean microdialysate concentration was 43 pg/mL in patients ‡ 65 years old, significantly higher compared to all other age groups ( p < 0.0001). Increased concentrations of glycerol and glutamate would indicate more extensive damaging processes in the elderly. An increase in concentration of FGF2 could serve a protective function, but could also be related to a dysregulation of the timing in the cellular response in elderly patients.

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Section: Sampling Of Microdialysatementioning

confidence: 80%

Section: Sampling Of Microdialysatementioning

confidence: 99%

Section: Sampling Of Microdialysatementioning

confidence: 99%

“…The study is part of an ongoing project, aimed at optimizing the care of NSICU patients (Mellergard et al, 2008(Mellergard et al, , 2010(Mellergard et al, , 2011.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Cerebral Extracellular Release of Glycerol, Glutamate, and FGF2 Is Increased in Older Patients following Severe Traumatic Brain Injury

Mellergård

Sjögren

Hillman

2012

Journal of Neurotrauma

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“…38 RANTES has also been collected from dialysis catheters that have been implanted into the human brain after TBI. 12,53 MIP-1α/CCL3 is a chemokine that serves to recruit and activate polymorphonuclear leukocytes. In this work, CCL3 was detected in most dialysates among the different groups.…”

mentioning

confidence: 99%

In Vivo Microdialysis Sampling of Cytokines from Rat Hippocampus: Comparison of Cannula Implantation Procedures

Vasicek

Jackson

Poseno

et al. 2013

ACS Chem. Neurosci.

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Cytokines are signaling proteins that have been of significant importance in the field of immunology, since these proteins affect different cells in the immune system. In addition to their immune system significance, these proteins have recently been referred to as a third chemical communication network within the CNS. The role that cytokines play in orchestrating the immune response within tissues after a mechanical injury leads to potential complications if the source of cytokines (i.e., trauma vs disease) is of interest. Microdialysis sampling has seen wide use in collection of many different solutes within the CNS. Yet, implantation of microdialysis guide cannulas and the probes creates tissue injury. In this study, we compared the differences in cytokine levels in dialysates from 4 mm, 100 kDa molecular weight cutoff (MWCO) polyethersulfone membrane microdialysis probes implanted in the hippocampus of male Sprague− Dawley rats. Comparisons were made between animals that were dialyzed immediately after cannula implantation (day 0), 7 days post cannula implantation (day 7), and repeatedly sampled on day 0 and day 7. Multiplexed bead-based immunoassays were used to quantify CCL2 (MCP-1), CCL3 (MIP-1α), CCL5 (RANTES), CXCL1 (KC/GRO), CXCL2 (MIP-2), IL-1β, IL-6, and IL-10 in dialysates. Differences in cytokine concentrations between the different treatment groups were observed with higher levels of inflammatory cytokines measured in day 7 cannulated animals. Only CCL3 (MIP-1α), CXCL1 (KC/GRO), CXCL2 (MIP-2), and IL-10 were measured above the assay limits of detection for a majority of the dialysates, and their concentrations were typically in the low to high (10−1000) picogram per milliliter range. The work described here lays the groundwork for additional basic research studies with microdialysis sampling of cytokines in rodent CNS.

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Microdialysis Use in Tumors: Drug Disposition and Tumor Response

Zhou

Gallo

2011

Applications of Microdialysis in Pharmaceutical Science

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Changes in Extracellular Concentrations of Some Cytokines, Chemokines, and Neurotrophic Factors After Insertion of Intracerebral Microdialysis Catheters in Neurosurgical Patients

Cited by 58 publications

References 43 publications

The Cerebral Extracellular Release of Glycerol, Glutamate, and FGF2 Is Increased in Older Patients following Severe Traumatic Brain Injury

The Cerebral Extracellular Release of Glycerol, Glutamate, and FGF2 Is Increased in Older Patients following Severe Traumatic Brain Injury

In Vivo Microdialysis Sampling of Cytokines from Rat Hippocampus: Comparison of Cannula Implantation Procedures

Microdialysis Use in Tumors: Drug Disposition and Tumor Response

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