Changes in electrophoretic mobilities of alpha-and beta-lipoproteins as a result of plasma delipidation.

Cham, Bill E.; Knowles, Brian R.

doi:10.1093/clinchem/22.3.305

Cited by 15 publications

(11 citation statements)

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“…Plasma proteins, including apolipoproteins, and other chemical constituents, and enzyme activities, remained stable in the delipidated plasma. Minor changes in electrophoretic mobilities after in vitro delipidation of these plasma lipoproteins have also been reported by our group [2]. Furthermore, apolipoproteins in delipidated plasma were capable of dissolving extracellular cholesterol [3] and were also able to mediate cholesterol efflux in a wide variety of cells including smooth muscle cells and macrophages [4-lo].…”

Section: Introductionsupporting

confidence: 59%

“…LA induced higher levels of lipid-stainable material in pre-P-HDL levels in both hypercholesterolemic and normocholesterolemic animals. However, although densitometric scans of lipidstainable materials have been shown to correlate with lipids in lipoproteins after electrophoresis [2], not all the lipoproteins stain equally.…”

Section: Discussionmentioning

confidence: 99%

“…It is known that plasma, delipidated with a butanol-DIPE mixture, retains apolipoproteins in solution [1][2][3][21][22][23][24][25][26][27][28][29]. In a recent study we have shown that reinfusion in normocholesterolemic animals [ 111.…”

Section: Discussionmentioning

confidence: 99%

“…All solvents and reagents used were analytical grade. The ethers were made peroxide free by distilling in the presence of ferrous sulfate [2].…”

Section: Materials and Methods Reagentsmentioning

confidence: 99%

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Lipid apheresis in an animal model causes in vivo changes in lipoprotein electrophoretic patterns

Cham

Kostner²,

Dwivedy³

et al. 1996

J. Clin. Apheresis

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Lipid apheresis, a new extracorporeal procedure based on plasma delipidation and showing promise as a possible treatment for atherosclerosis, was recently reported for the first time from this laboratory [Cham et al., J Clin Apheresis 10:61–69. 1995]. In the present study lipid apheresis was applied to hypercholesterolemic and normocholesterolemic roosters to examine its effect on plasma lipoprotein particles. This procedure resulted in conspicuous changes in electrophoretic patterns of plasma lipoproteins. The electrophoretic mobilities of all the lipoprotein fractions had changed considerably. Lipid stainable material was present in at least three bands in the α‐globulin area. In particular, changes in the electrophoretic region of high‐density lipoproteins were observed. Lipid apheresis markedly induced the anti‐atherogenic pre‐β‐high‐density lipoproteins. The observed changes induced by lipid apheresis were more pronounced in the hyperlipidemic animals compared with the normocholesterolemic controls. A novel pre‐α‐lipoprotein band was observed soon after lipid apheresis. This lipoprotein band had a density larger than 1.21. At approximately 150 minutes after lipid apheresis, the electrophoretic pattern had almost returned to its original base pattern. Lipid apheresis results in plasma lipoprotein changes which may induce reverse cholesterol transport and shows promise as a possible treatment of atherosclerosis. © 1996 Wiley‐Liss, Inc.

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Section: Introductionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…All solvents and reagents used were analytical grade. The ethers were made peroxide free by distilling in the presence of ferrous sulfate [2].…”

Section: Materials and Methods Reagentsmentioning

confidence: 99%

See 2 more Smart Citations

Lipid apheresis in an animal model causes in vivo changes in lipoprotein electrophoretic patterns

Cham

Kostner²,

Dwivedy³

et al. 1996

J. Clin. Apheresis

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“…It has also been shown that PDP results in ordered delipidation. In plasma, HDL is first delipidated followed by VLDL and LDL [48]. All apolipoproteins in plasma are retained after PDP treatment, and the net effect of these apolipoproteins is RCT.…”

Section: Discussionmentioning

confidence: 99%

Plasma delipidation process induces rapid regression of atherosclerosis and mobilisation of adipose tissue

et al. 2005

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Cholesterol is a major component of atherosclerotic plaques. Cholesterol accumulation within the arterial intima and atherosclerotic plaques is determined by the difference of cellular cholesterol synthesis and/or influx from apo B-containing lipoproteins and cholesterol efflux. In humans, apo A-1 Milano infusion has led to rapid regression of atherosclerosis in coronary arteries. We hypothesised that a multifunctional plasma delipidation process (PDP) would lead to rapid regression of experimental atherosclerosis and probably impact on adipose tissue lipids. In hyperlipidemic animals, the plasma concentrations of cholesterol, triglyceride and phospholipid were, respectively, 6-, 157-, and 18-fold higher than control animals, which consequently resulted in atherosclerosis. PDP consisted of delipidation of plasma with a mixture of butanol-diisopropyl ether (DIPE). PDP removed considerably more lipid from the hyperlipidemic animals than in normolipidemic animals. PDP treatment of hyperlipidemic animals markedly reduced intensity of lipid staining materials in the arterial wall and led to dramatic reduction of lipid in the adipose tissue. Five PDP treatments increased apolipoprotein A1 concentrations in all animals. Biochemical and hematological parameters were unaffected during PDP treatment. These results show that five PDP treatments led to marked reduction in avian atherosclerosis and removal of lipid from adipose tissue. PDP is a highly effective method for rapid regression of atherosclerosis.

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Lipid apheresis: An in vivo application of plasma delipidation with organic solvents resulting in acute transient reduction of circulating plasma lipids in animals

Cham

Kostner

Dwivedy

et al. 1995

J of Clinical Apheresis

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Despite primary and secondary prevention of coronary disease with lowering plasma cholesterol by diet and drug therapy, coronary heart disease remains the major cause of death in Western countries. Low density lipoprotein apheresis had the potential to make a significant impact as it acutely leads to a marked reduction in plasma cholesterol. However, recent preliminary results suggest that low density lipoprotein apheresis may not be more effective in preventing progression of coronary disease than current drug therapy. We have devised a new technique, termed lipid apheresis, which removes cholesterol and triglycerides from plasma but retains the apolipoproteins. This procedure shows great promise in stimulating regression beyond current therapy. Lipid apheresis, a new extracorporeal procedure based on plasma delipidation with the organic solvent mixture butanol-diisopropyl ether, was applied to hypercholesterolemic and normocholesterolemic roosters. Approximately 25% of the calculated blood volume was removed from the animals. The plasma was separated from the blood cells. The plasma was delipidated for 20 min with the organic solvent mixture. The delipidated plasma containing all proteins, including the apolipoproteins and other ionic constituents, was remixed with the blood cells and infused back into the identical donor animals. Analyses of serial blood samples collected from lipid apheresed and sham treated animals up to 16 h after infusion revealed that lipid apheresis caused acute, marked reductions in plasma lipids. The pattern and extent of the plasma levels of cholesterol were different in the hypercholesterolemic animals when compared with normocholesterolemic animals, indicating that a readily extraplasma cholesterol pool in the hypercholesterolemic animals was rapidly mobilized into the plasma pool.(ABSTRACT TRUNCATED AT 250 WORDS)

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Changes in electrophoretic mobilities of alpha-and beta-lipoproteins as a result of plasma delipidation.

Cited by 15 publications

References 0 publications

Lipid apheresis in an animal model causes in vivo changes in lipoprotein electrophoretic patterns

Lipid apheresis in an animal model causes in vivo changes in lipoprotein electrophoretic patterns

Plasma delipidation process induces rapid regression of atherosclerosis and mobilisation of adipose tissue

Lipid apheresis: An in vivo application of plasma delipidation with organic solvents resulting in acute transient reduction of circulating plasma lipids in animals

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