Changes in electric charge and phospholipids composition in human colorectal cancer cells

Abstract

Cancer cells perform their malicious activities through own cell membranes that screen and transmit inhibitory and stimulatory signals out of the cells and into them. This work is focused on changes of phospholipids content (PI-phosphatidylinositol, PS-phosphatidylserine, PE-phosphatidylethanolamine, PC-phosphatidylcholine) and electric charge that occur in cell membranes of colorectal cancer of pT 3 stage, various grades (G2, G3) and without/with metastasis. Qualitative and quantitative composition of phospholipids in the membrane was determined by HPLC (high-performance liquid chromatography). The surface charge density of colorectal cancer cell membranes was measured using electrophoresis. The measurements were carried out at various pH of solution. It was shown that the process of cancer transformation was accompanied by an increase in total amount of phospholipids as well as an increase in total positive charge at low pH and total negative charge at high pH. A malignant neoplasm cells with metastases are characterized by a higher PC/PE ratio than malignant neoplasm cells without metastases.

The roles of macromolecules in imatinib resistance of chronic myeloid leukemia cells by Fourier transform infrared spectroscopy

“…Decreases in both the ratios indicate an increase in the total amount of phosphate compounds in the imatinib resistant cancer cells. In cancer cell formation, the phospholipid molecules and their metabolites are believed to participate in the oncogene-induced transformation processes [44] and protein phosphorylation is known to be fostered [45]. These results also agree with the increase in the intensity of the shoulder around 1170 cm À1 in the imatinib resistant cancer cells indicating protein phosphorylation [46].…”

Effect of low voltage AC fields on cardiovascular implants

“…Sodium and Chlorine ions have been reported to be at elevated levels in tumour cells [21], which results in their lower polarity values. Several methods of quantifying ion levels have been reported over the years [20][21][22], more recent methods include electrophoresis [22]. Hence, altering ion channel activity can regulate the voltage membrane potential to promote normal cell differentiation and cell activity.…”

Untitled

“…The lytic action of magainin II is due to the presence of net positive charges of magainin that interacts with the negative charges located in the outer leaflet of the cancer cell membrane 20 . O-glycosylated mucin, a type of glycoprotein, exists in the cancer cell membranes that contribute to the negative charges on the cancer cell surfaces 21 .…”