Changes in DNA-methylation during zygotic embryogenesis in interspecific hybrids of beans (Phaseolus ssp.)

Abid, Ghassen; Muhoviski, Yordan; Jacquemin, Jean-Marie; Mingeot, Dominique; Sassı, Khaled; Toussaint, André; Baudoin, Jean-Pierre

doi:10.1007/s11240-010-9878-2

Cited by 14 publications

(8 citation statements)

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“…DNA methylation is generally recognized to suppress gene expression as regulatory factors, homozygosity/heterozygosity of methylated DNA may be involved in inbreeding depression/heterosis (Nakamura and Hosaka, 2010). A considerable change in the methylation pattern is critical during embryogenesis process and gene expression such as in Phaseolus interspecific hybrids and could be involved in the disruption of the regulation or maintenance of the embryogenesis (Abid et al 2011). This provides further insight into the molecular mechanisms involved epigenetic variations in the somatic hybrids regeneration by more molecular markers.…”

Section: Discussionmentioning

confidence: 99%

“…The AFLP and the MSAP markers have been used to detect significant genetic and epigenetic changes, respectively in a number of crop species for example potato (Joyce and Cassells, 2002;Dann and Wilson, 2011), Solanum aculeatissimum (Ghimire et al 2012), Triticum aestivum (Meng et al 2012), Gardenia jasminoides (Wu et al 2012), Ungernia victoris (Bublyk et al 2012), Capparis spinosa (Carra et al 2012), Phaseolus ssp. (Abid et al 2011), Nicotiana tabacum (Yang et al 2011), Freesia hybrida (Gao et al 2010), Ocotea catharinensis (Hanai et al 2010), Cymbidium (Chen et al 2009), Vitis spp. (Baránek et al 2010), Brassica oleracea (Salmon et al 2008), Hordeum brevisubulatum (Li et al 2007) and Humulus lupulus (Peredo et al 2006).…”

Section: Introductionmentioning

confidence: 99%

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Analysis of genetic and epigenetic variation in in vitro propagated potato somatic hybrid by AFLP and MSAP marker

Tiwari¹,

Saurabh²,

Chandel³

et al. 2013

Electron. J. Biotechnol.

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Background: Genetic and epigenetic changes (DNA methylation) were examined in the tissue-culture propagated interspecific potato somatic hybrids between dihaploid Solanum tuberosum and S. pinnatisectum. Amplified fragment length polymorphism (AFLP) and methylation-sensitive amplified polymorphism (MSAP) were applied to detect the genetic and epigenetic changes, respectively in the somatic hybrids mother plants (1 st cycle) and their regenerants (30 th cycles sub-cultured). Results: To detect genetic changes, eight AFLP primer combinations yielded a total of 329 scorable bands of which 49 bands were polymorphic in both mother plants and regenerants. None of the scorable bands were observed in term of loss of original band of mother plant or gain of novel band in their regenerants. AFLP profiles and their cluster analysis based on the Jaccard's similarity coefficient revealed 100% genetic similarity among the mother plant and their regenerants. On the other hand, to analyze epigenetic changes, eight MSAP primer pair combinations detected a few DNA methylation patterns in the mother plants (0 to 3.4%) and their regenerants (3.2 to 8.5%). Out of total 2320 MSAP sites in the mother plants, 2287 (98.6%) unmethylated, 21 (0.9%) fully methylated and 12 (0.5%) hemimethylated, and out of total 2494 MSAP sites in their regenerants, 2357 (94.5%) unmethylated, 79 (3.1%) fully methylated and 58 (2.3%) hemi-methylated sites were amplified. Conclusion: The study concluded that no genetic variations were observed among the somatic hybrids mother plants and their regenerants by eight AFLP markers. However, minimum epigenetic variations among the samples were detected ranged from 0 to 3.4% (mother plants) and 3.2 to 8.5% (regenerants) during the tissue culture process.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysis of genetic and epigenetic variation in in vitro propagated potato somatic hybrid by AFLP and MSAP marker

Tiwari¹,

Saurabh²,

Chandel³

et al. 2013

Electron. J. Biotechnol.

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“…Seed dormancy and germination are complex physiological processes that are controlled by genetic factors (Abid et al 2011) and developmental and external cues (Koornneef et al 2002;Uma et al 2010). Germination begins with water uptake by seeds and terminates with the initial elongation of the embryonic axis (Bewley 1997;Panaia et al 2009).…”

Section: Introductionmentioning

confidence: 99%

GA3 induces expression of E2F-like genes and CDKA during in vitro germination of zygotic embryos of Cocos nucifera (L.)

Montero-Cortés

Cordova

Verdeil

et al. 2011

Plant Cell Tiss Organ Cult

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Previous reports have demonstrated that gibberellins promote seed germination, including in vitro germination of zygotic embryos of coconut. Genes that control the cell cycle seem to play important roles during germination. In this study, complete full-length sequences of two E2F-like genes, CnE2F1 and CnE2F2, were obtained. Deduced amino acid sequences of both genes showed conserved domains characteristic of E2F genes. Both genes showed high homology with Arabidopsis thaliana E2Fa and E2Fb, which are transcriptional activators. The expression profiles of CnE2F genes were evaluated in in vitro zygotic embryos with or without gibberellic acid. In control embryos, expression of CnE2F1 showed a peak at day 2, and, gradually this dropped to similar levels as those at day 0. Gibberellic acid (GA3) treatment promoted expression at day 2, higher than that of the control. For CnE2F2, peak expression was detected at day 8 of culture. However, with GA treatment, CnE2F2 expression profile increased by day 2. The expression profile of a cyclin-dependent kinase (CnCDKA), another gene related to the cell cycle control, was also studied. In control embryos, expression of this gene showed two peaks at days 2 and 8, compared with day 0. A different profile was shown with GA treatment. In general, major changes in expression profiles were observed from days 2-8 when morphological changes in zygotic embryos were not yet observed.

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“…We found 1.5 times more informative genmarkers within the A genome compared with the C. This may reflect the breeding pedigree of the Ningyou7DH parents, where B. rapa (AA) contributed as a recent ancestor, and de novo DNA methylation may have arisen during introgression to the A genome of B. napus. Hybrids from interspecific crosses in Arabidopsis (Bushell et al 2003) and cotton (Keyte et al 2006) show changes in the DNA methylation pattern compared with parents, and de novo methylation and demethylation occur in interspecific hybrids of beans (Abid et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Epigenetic QTL Mapping inBrassica napus

Long

Xia

et al. 2011

Genetics

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There is increasing evidence that epigenetic marks such as DNA methylation contribute to phenotypic variation by regulating gene transcription, developmental plasticity, and interactions with the environment. However, relatively little is known about the relationship between the stability and distribution of DNA methylation within chromosomes and the ability to detect trait loci. Plant genomes have a distinct range of target sites and more extensive DNA methylation than animals. We analyzed the stability and distribution of epialleles within the complex genome of the oilseed crop plant Brassica napus. For methylation sensitive AFLP (MSAP) and retrotransposon (RT) epimarkers, we found a high degree of stability, with 90% of mapped markers retaining their allelic pattern in contrasting environments and developmental stages. Moreover, for two distinct parental lines 97% of epialleles were transmitted through five meioses and segregated in a mapping population. For the first time we have established the genetic position for 17 of the 19 centromeres within this amphidiploid species. Epiloci and genetic loci were distributed within distinct clusters, indicating differential detection of recombination events. This enabled us to identify additional significant QTL associated with seven important agronomic traits in the centromeric regions of five linkage groups.

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Changes in DNA-methylation during zygotic embryogenesis in interspecific hybrids of beans (Phaseolus ssp.)

Cited by 14 publications

References 50 publications

Analysis of genetic and epigenetic variation in in vitro propagated potato somatic hybrid by AFLP and MSAP marker

Analysis of genetic and epigenetic variation in in vitro propagated potato somatic hybrid by AFLP and MSAP marker

GA3 induces expression of E2F-like genes and CDKA during in vitro germination of zygotic embryos of Cocos nucifera (L.)

Epigenetic QTL Mapping inBrassica napus

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