Changes in cytokinin levels and metabolism in tobacco (Nicotiana tabacum L.) explants during in vitro shoot organogenesis induced by trans-zeatin and dihydrozeatin

Klemš, Marek; Slámová, Zdeňka; Motyka, Václav; Malbeck, Jiří; Trávníčková, Alena; Macháčková, Ivana; Holı́k, Josef; Procházka, Stanislav

doi:10.1007/s10725-011-9612-z

Cited by 12 publications

(7 citation statements)

References 48 publications

(54 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The increase in the total CK pool after exogenous CK treatment was due to increases in the abundance of different metabolites, which depended on the type of CK used, the concentration applied (Bairu et al ) and/or the culture stage being studied (Klemš et al ).…”

Section: Discussionmentioning

confidence: 68%

“…The simultaneous occurrence of CK bases with the corresponding nucleosides and nucleotides in plant tissues suggests that the metabolic flow from CK nucleotides to the active bases is probably circular rather than unidirectional (Sakakibara ). Several authors have argued that the free bases are the physiologically active species, with the ribosides and ribotides being transport forms (Klemš et al ). In addition, CK glucosylation has been observed at the N3, N7 and N9 positions of the purine moiety (generating N ‐glucosides) and at the hydroxyl group of the side chain (generating O ‐glucosides).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Endogenous cytokinin and auxin profiles during in vitro organogenesis from vegetative buds of Pinus radiata adult trees

Montalbán

Novák

Rolčı́k

et al. 2012

Physiologia Plantarum

View full text Add to dashboard Cite

In Pinus radiata D. Don, the transition from the juvenile to the mature phase is characterized by a reduction in the tree's organogenic potential, which is usually reverted in breeding programs by reinvigoration procedures to enable vegetative propagation. In this work, we have determined the best culture conditions for in vitro reinvigoration of radiata pine buds, tested different cytokinin (CK) types [N⁶-benzyladenine (BA), meta-topolin (mT) and trans-zeatin] and concentrations (25 and 50 µM), and studied the effect of culture conditions on endogenous CK and indole-3-acetic acid (IAA) levels at different stages of the organogenic process. To this end, the levels of 43 CKs and IAA were determined in P. radiata buds before and during the reinvigoration process. When BA or mT was applied to the induction medium, we did not observe any significant increase or decrease in endogenous isoprenoid CK content. We also report for the first time the presence of O-glucosides in non-treated P. radiata explants from the field and remark the importance of O-glucosides as storage forms.

show abstract

Section: Discussionmentioning

confidence: 68%

Section: Introductionmentioning

confidence: 99%

Endogenous cytokinin and auxin profiles during in vitro organogenesis from vegetative buds of Pinus radiata adult trees

Montalbán

Novák

Rolčı́k

et al. 2012

Physiologia Plantarum

View full text Add to dashboard Cite

show abstract

“…Highest amount of bases, the active forms of cytokinins (Klemš et al, 2011), were found in Se developed under 18 and 23°C and low gellan gum concentration, treatments showing the highest water availability. So, our results are in agreement to other authors describing that a decrease in water availability could be related to the rapidly utilization of CK bases and tightly regulated by mechanisms that prevent their accumulation at high levels (Moncaleán et al, 2005; Stirk et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Temperature and Water Availability During Maturation Affect the Cytokinins and Auxins Profile of Radiata Pine Somatic Embryos

et al. 2018

View full text Add to dashboard Cite

Somatic embryogenesis (SE) provides us a potent biotechnological tool to manipulate the physical and chemical conditions (water availability) along the process and to study their effect in the final success in terms of quantity of somatic embryos produced. In the last years, our research team has been focused on the study of different aspects of the SE in Pinus spp. One of the main aspects affecting SE is the composition of culture media; in this sense, phytohormones play one of the most crucial roles in this propagation system. Many studies in conifers have shown that different stages of SE and somatic embryo development are correlated with distinct endogenous phytohormone profiles under the stress conditions needed for the process (i.e., cytokinins play a regulatory role in stress signaling, which it is essential for radiata pine SE). Based on this knowledge, the aim of this study was to test the effect of different temperatures (18, 23, and 28°C) and gelling agent concentrations (8, 9, and 10 gL-1) during the maturation stage of Pinus radiata SE in maturation and germination rates. Parallel, phytohormone profile of somatic embryos developed was evaluated. In this sense, the highest gellan gum concentration led to significantly lower water availability. At this gellan gum concentration and 23°C a significantly higher number of somatic embryos was obtained and the overall success of the process increased with respect to other treatments assayed. The somatic embryos produced in these conditions showed the highest concentration of iP-type cytokinins and total ribosides. Although, the different conditions applied during maturation of somatic embryos led to different hormonal profiles, they did not affect the ex vitro survival of the resulting somatic plants, where no significant differences were observed.

show abstract

“…This variation among genotypes might be related to the level of endogenous hormones [30] or the effect of exogenous growth regulators on the level of endogenous hormones through influencing their biosynthesis and distribution [31], which subsequently alters the in vitro regeneration responses [32][33][34]. Interestingly, all three improved tef varieties used in the current study had extremely poor regeneration capacity.…”

Section: Discussionmentioning

confidence: 83%

Efficiency of In Vitro Regeneration is Dependent on the Genotype and Size of Explant in Tef [Eragrostis tef (Zucc.) Trotter]

Plaza-Wüthrich

Blösch²,

Tadele³

2015

Adv Crop Sci Tech

View full text Add to dashboard Cite

Tef [Eragrostis tef (Zucc.) Trotter] is the major cereal crop in the Horn of Africa particularly in Ethiopia where it is staple food for about 50 million people. Its resilience to extreme environmental conditions and high in nutrition makes tef the preferred crop among both farmers and consumers. The efficiency of in vitro regeneration plays significant role in the improvement of crops. We investigated the efficiency of regeneration in 18 tef genotypes (15 landraces and three improved varieties) using three sizes of immature embryos (small, intermediate and large) as an explant. In vitro regeneration was significantly affected by the genotype and the size of the immature embryo used as a donor. Intermediate-size immature embryos which were 101-350 µm long led to the highest percentage of regeneration. Interestingly, the three improved varieties presented very low regeneration efficiencies whereas the landrace Manyi resulted in consistently superior percentage of in vitro regeneration from all three sizes of explants. The findings of this work provide useful insight into the tef germplasm amenable for the regeneration technique which has direct application in techniques such as transformation. It also signifies the importance of using tef landraces instead of improved varieties for in vitro regeneration.

show abstract

Changes in cytokinin levels and metabolism in tobacco (Nicotiana tabacum L.) explants during in vitro shoot organogenesis induced by trans-zeatin and dihydrozeatin

Cited by 12 publications

References 48 publications

Endogenous cytokinin and auxin profiles during in vitro organogenesis from vegetative buds of Pinus radiata adult trees

Endogenous cytokinin and auxin profiles during in vitro organogenesis from vegetative buds of Pinus radiata adult trees

Temperature and Water Availability During Maturation Affect the Cytokinins and Auxins Profile of Radiata Pine Somatic Embryos

Efficiency of In Vitro Regeneration is Dependent on the Genotype and Size of Explant in Tef [Eragrostis tef (Zucc.) Trotter]

Contact Info

Product

Resources

About